Chicken Recombinant Monoclonal Neurofilament heavy polypeptide antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.
IgY
Chicken
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
---|---|---|---|---|
Human | Tested | Tested | Tested | Not recommended |
Mouse | Tested | Tested | Tested | Not recommended |
Rat | Tested | Tested | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Species Mouse | Dilution info 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
KIAA0845, NFH, NFH, KIAA0845, NEFH, Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein
Chicken Recombinant Monoclonal Neurofilament heavy polypeptide antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.
IgY
Chicken
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR20020
Affinity purification Thiophilic Resin
Unsuitable for rat ICC. WB not recommend cell lysate.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This Chicken monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-Neurofilament heavy polypeptide antibody [EPR20020] ab207176). By design, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
NEFH also known as Neurofilament Heavy Chain or NEF3 is a structural protein with a molecular mass of approximately 112 kDa. This protein is primarily expressed in neurons especially within the axons. NEFH plays a mechanical role in maintaining the structural stability and caliber of axons. It is a major constituent of the axoskeleton and contributes to the maintenance of neuronal shape. NEFH acts as a scaffold allowing other proteins to assemble onto it forming neurofilament complexes that provide support to neuronal cells.
Neurofilament proteins like NEFH contribute to the development and maintenance of neuron shape. NEFH exists as part of a larger neurofilament complex which includes two other major neurofilament subunits: NEFL (neurofilament light) and NEFM (neurofilament medium). Together these protein components form long filaments giving neurons their tensile strength. NEFH is important in dictating the radial growth of axons facilitating efficient nerve conduction.
Proteins involved in axonal transport and cytoskeletal organization engage with NEFH. In the axonal transport pathway NEFH associates with proteins such as dynein and kinesin necessary for the movement of organelles along the axonal microtubules. Furthermore NEFH's role in the cytoskeletal organization pathway involves interaction with actin and microtubule-associated proteins to ensure proper neuron function and structural integrity.
Neurodegenerative conditions such as Amyotrophic Lateral Sclerosis (ALS) and some forms of Charcot-Marie-Tooth disease link NEFH involvement. Accumulation of NEFH in motor neurons has been observed in ALS indicating disruption in axonal transport. In these diseases alterations in NEFH protein interactions such as with NEFL can lead to disrupted neurofilament integrity and axonal stability contributing to neuronal dysfunction and disease progression.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labelling NEFH with ab317042 at 1/500 (2.098 ug/ml) dilution, followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).
Confocal image showing cytoplasmic staining in SH-SY5Y cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Negative control: MCF7 (PMID: 25985363).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling NEFH with ab317042 at 1/500 (2.098 ug/ml) dilution, followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).
Confocal image showing positive staining in mouse primary neuron (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/200 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
-ve control 1: ab317042 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
-ve control 2: Anti-MAP2 antibody [HM-2] ab11267 at 1/200 dilution, followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling NEFH with ab317042 at 1/10000 (0.105 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human cerebrum.
The section was incubated with 317042 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins
Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling NEFH with ab317042 at 1/10000 (0.105 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human prostatic hyperplasia.
The section was incubated with 317042 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling NEFH with ab317042 at 1/10000 (0.105 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse cerebrum.
The section was incubated with 317042 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NEFH with ab317042 at 1/10000 (0.105 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human kidney.
The section was incubated with 317042 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling NEFH with ab317042 at 1/10000 (0.105 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat cerebrum.
The section was incubated with 317042 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins
Negative control: kidney
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
Exposure time: Lanes 1-2: 10 seconds, Lanes 3-8: 6 seconds
All lanes: Western blot - Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) (ab317042) at 1/1000 dilution
Lane 1: Human Brainstem tissue lysate at 20 µg
Lane 2: Human kidney tissue lysate at 20 µg
Lane 3: Mouse cerebellum tissue lysate at 20 µg
Lane 4: Mouse brain tissue lysate at 20 µg
Lane 5: Mouse kidney tissue lysate at 20 µg
Lane 6: Rat cerebellum tissue lysate at 20 µg
Lane 7: Rat brain tissue lysate at 20 µg
Lane 8: Rat kidney tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (Goat Anti-Chicken IgY H&L (HRP) ab6877) at 1/100000 dilution
Observed band size: 200-210 kDa, 124 kDa
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