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Chicken Recombinant Monoclonal Neurofilament heavy polypeptide antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) (AB317042), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) (AB317042), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) (AB317042), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) (AB317042), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) (AB317042), expandable thumbnail

Key facts

Isotype

IgY

Host species

Chicken

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Not recommended
Mouse
Tested
Tested
Tested
Not recommended
Rat
Tested
Tested
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/10000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/10000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Species

Mouse

Dilution info

1/500

Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

Alternative names

Recommended products

Chicken Recombinant Monoclonal Neurofilament heavy polypeptide antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgY

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR20020

Purification technique

Affinity purification Thiophilic Resin

Specificity

Unsuitable for rat ICC. WB not recommend cell lysate.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This Chicken monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-Neurofilament heavy polypeptide antibody [EPR20020] ab207176). By design, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

NEFH also known as Neurofilament Heavy Chain or NEF3 is a structural protein with a molecular mass of approximately 112 kDa. This protein is primarily expressed in neurons especially within the axons. NEFH plays a mechanical role in maintaining the structural stability and caliber of axons. It is a major constituent of the axoskeleton and contributes to the maintenance of neuronal shape. NEFH acts as a scaffold allowing other proteins to assemble onto it forming neurofilament complexes that provide support to neuronal cells.

Biological function summary

Neurofilament proteins like NEFH contribute to the development and maintenance of neuron shape. NEFH exists as part of a larger neurofilament complex which includes two other major neurofilament subunits: NEFL (neurofilament light) and NEFM (neurofilament medium). Together these protein components form long filaments giving neurons their tensile strength. NEFH is important in dictating the radial growth of axons facilitating efficient nerve conduction.

Pathways

Proteins involved in axonal transport and cytoskeletal organization engage with NEFH. In the axonal transport pathway NEFH associates with proteins such as dynein and kinesin necessary for the movement of organelles along the axonal microtubules. Furthermore NEFH's role in the cytoskeletal organization pathway involves interaction with actin and microtubule-associated proteins to ensure proper neuron function and structural integrity.

Associated diseases and disorders

Neurodegenerative conditions such as Amyotrophic Lateral Sclerosis (ALS) and some forms of Charcot-Marie-Tooth disease link NEFH involvement. Accumulation of NEFH in motor neurons has been observed in ALS indicating disruption in axonal transport. In these diseases alterations in NEFH protein interactions such as with NEFL can lead to disrupted neurofilament integrity and axonal stability contributing to neuronal dysfunction and disease progression.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) (ab317042), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) (ab317042)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labelling NEFH with ab317042 at 1/500 (2.098 ug/ml) dilution, followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

    Confocal image showing cytoplasmic staining in SH-SY5Y cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Negative control: MCF7 (PMID: 25985363).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) (ab317042), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) (ab317042)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling NEFH with ab317042 at 1/500 (2.098 ug/ml) dilution, followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

    Confocal image showing positive staining in mouse primary neuron (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/200 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    -ve control 1: ab317042 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.

    -ve control 2: Anti-MAP2 antibody [HM-2] ab11267 at 1/200 dilution, followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) (ab317042), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) (ab317042)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling NEFH with ab317042 at 1/10000 (0.105 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human cerebrum.

    The section was incubated with 317042 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) (ab317042), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) (ab317042)

    Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling NEFH with ab317042 at 1/10000 (0.105 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human prostatic hyperplasia.

    The section was incubated with 317042 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) (ab317042), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) (ab317042)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling NEFH with ab317042 at 1/10000 (0.105 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse cerebrum.

    The section was incubated with 317042 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) (ab317042), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) (ab317042)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NEFH with ab317042 at 1/10000 (0.105 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on human kidney.

    The section was incubated with 317042 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) (ab317042), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) (ab317042)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling NEFH with ab317042 at 1/10000 (0.105 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat cerebrum.

    The section was incubated with 317042 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Western blot - Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) (ab317042), expandable thumbnail

    Western blot - Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) (ab317042)

    Negative control: kidney

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    Exposure time: Lanes 1-2: 10 seconds, Lanes 3-8: 6 seconds

    All lanes: Western blot - Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) (ab317042) at 1/1000 dilution

    Lane 1: Human Brainstem tissue lysate at 20 µg

    Lane 2: Human kidney tissue lysate at 20 µg

    Lane 3: Mouse cerebellum tissue lysate at 20 µg

    Lane 4: Mouse brain tissue lysate at 20 µg

    Lane 5: Mouse kidney tissue lysate at 20 µg

    Lane 6: Rat cerebellum tissue lysate at 20 µg

    Lane 7: Rat brain tissue lysate at 20 µg

    Lane 8: Rat kidney tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (Goat Anti-Chicken IgY H&L (HRP) ab6877) at 1/100000 dilution

    Observed band size: 200-210 kDa, 124 kDa

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Product protocols

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