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AB110049

Anti-NEIL3 antibody

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Rabbit Polyclonal NEIL3 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human NEIL3.

View Alternative Names

Endonuclease 8-like 3, DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, Endonuclease VIII-like 3, Nei-like protein 3, NEIL3

1 Images
Western blot - Anti-NEIL3 antibody (AB110049)
  • WB

Unknown

Western blot - Anti-NEIL3 antibody (AB110049)

All lanes:

Western blot - Anti-NEIL3 antibody (ab110049) at 1/500 dilution

Lane 1:

COLO cell extract at 30 µg

Lane 2:

HuvEc cell extract at 30 µg

Lane 3:

HepG2 cell extract at 30 µg

Lane 4:

HeLa cell extract at 30 µg

Lane 5:

HuvEc cell extract at 30 µg with immunising peptide

Predicted band size: 68 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human NEIL3. The exact immunogen used to generate this antibody is proprietary information.

Q8TAT5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500.00000 - 1/1000.00000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NEIL3 also called Nei-like DNA glycosylase 3 plays an important role in DNA repair processes. It is a DNA glycosylase enzyme that weighs approximately 66 kDa. NEIL3 removes oxidized bases from DNA and is expressed in high levels in the thymus testis and brain tissues. The enzyme performs base excision repair which involves recognizing and excising damaged bases thereby maintaining genomic stability.
Biological function summary

NEIL3 participates in the maintenance of an organism's genome integrity. It works as a part of the base excision repair complex interacting with other proteins to repair DNA damage caused by oxidation and alkylation. The enzyme interacts with proliferating cell nuclear antigen (PCNA) to process damage during DNA replication. Its ability to handle a wide range of oxidative lesions is significant for cellular response to DNA damage.

Pathways

NEIL3 is closely integrated into the base excision repair and DNA replication pathways. The base excision repair pathway finds NEIL3 working alongside other DNA glycosylases like OGG1 and NTHL1. It also connects with components like DNA polymerase beta (POLB) for excision and repair. In the DNA replication pathway NEIL3 interacts with DNA replication machinery to maintain replication fork stability collaborating with proteins like RAD51 for homologous recombination and repair processes.

Researchers link NEIL3 to cancer and neurological diseases. Alterations in NEIL3 expression or function often result in genomic instability contributing to oncogenesis. Studies find links between NEIL3 malfunction and certain brain disorders where it may interact with Alzheimer's disease-related proteins like amyloid precursor protein (APP). Targeting NEIL3 pathways could provide therapeutic routes for treating these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA) (PubMed : 12433996, PubMed : 19170771, PubMed : 22569481, PubMed : 23755964). Mediates interstrand cross-link repair in response to replication stress : acts by mediating DNA glycosylase activity, cleaving one of the two N-glycosyl bonds comprising the interstrand cross-link, which avoids the formation of a double-strand break but generates an abasic site that is bypassed by translesion synthesis polymerases (By similarity). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA (PubMed : 12433996, PubMed : 19170771, PubMed : 22569481, PubMed : 23755964). No activity on 8-oxoG detected (PubMed : 12433996, PubMed : 19170771, PubMed : 22569481, PubMed : 23755964). Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity (PubMed : 12433996, PubMed : 19170771, PubMed : 22569481, PubMed : 23755964). In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues (PubMed : 12433996, PubMed : 19170771, PubMed : 22569481, PubMed : 23755964).
See full target information NEIL3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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