Anti-NEK1 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NEK1 antibody (AB229489)

4% paraformaldehyde-fixed U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells stained for NEK1 (green) using ab229489 at 1/500 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue).

• WB

Collaborator

Western blot - Anti-NEK1 antibody (AB229489)

ab229489 was shown to react with NEK1 in wild-type HeLa cells in Western blot with loss of signal observed in a NEK1 knockout cell line. Wild-type HeLa and NEK1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab229489 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 1/5000 before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-NEK1 antibody (ab229489)

Predicted band size: 142 kDa

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• WB

Supplier Data

Western blot - Anti-NEK1 antibody (AB229489)

5% SDS-PAGE

All lanes:

Western blot - Anti-NEK1 antibody (ab229489) at 1/1000 dilution

All lanes:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Predicted band size: 142 kDa

true

• WB

Supplier Data

Western blot - Anti-NEK1 antibody (AB229489)

Samples were separated by 5 % SDS. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-NEK1 antibody (ab229489) at 1/1000 dilution

Lane 1:

Daudi whole cell extract at 30 µg

Lane 2:

HeLa whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 143 kDa

false

• WB

Supplier Data

Western blot - Anti-NEK1 antibody (AB229489)

5% SDS-PAGE

All lanes:

Western blot - Anti-NEK1 antibody (ab229489) at 1/1000 dilution

Lane 1:

Mouse brain tissue extract at 50 µg

Lane 2:

Rat brain tissue extract at 50 µg

Predicted band size: 142 kDa

true