Rabbit Polyclonal NEK2 antibody. Suitable for IHC-P, ELISA, WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human NEK2 aa 250-300 conjugated to Keyhole Limpet Haemocyanin.
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- Frontiers in oncology 11:6777632021NEK2 Promotes Cell Proliferation and Glycolysis by Regulating PKM2 Abundance Phosphorylation in Diffuse Large B-Cell Lymphoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingna Zhou,Liya Ding,Yuqi Gong,Jing Zhao,Jing Zhang,Zhengrong Mao,Zhe Wang,Wei Zhang,Ren ZhouPubMed 34168996
- Journal of Cancer 12:2083-20912021NEK2 regulates cellular proliferation and cabergoline sensitivity in pituitary adenomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFangfang Jian,Yuhao Sun,Qingfang Sun,Benyan Zhang,Liuguan BianPubMed 33754007
- Journal of cellular and molecular medicine 24:7353-73692020microRNA-128-3p overexpression inhibits breast cancer stem cell characteristics through suppression of Wnt signalling pathway by down-regulating NEK2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuanwen Chen et. al.PubMed 32558224
- Frontiers in bioengineering and biotechnology 8:2592020Exosome-Derived miR-486-5p Regulates Cell Cycle, Proliferation and Metastasis in Lung Adenocarcinoma via Targeting NEK2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuihui Hu,Hangdi Xu,Fen Lu,Jisong Zhang,Li Xu,Shan Xu,Hanliang Jiang,Qingxin Zeng,Enguo Chen,Zhengfu HePubMed 32322578
- IUBMB life 71:1355-13662019microRNA-138 induces cell survival and reduces WNT/β-catenin signaling of osteoarthritis chondrocytes through NEK2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiling Xu et. al.PubMed 31034758
- International journal of cancer 140:1581-15962017Hepatoma cell functions modulated by NEK2 are associated with liver cancer progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSheng-Ming Wu et. al.PubMed 27925179
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NEK2 aa 250-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P51955
Reactivity data
Select an application| IHC-P | ELISA | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Expected | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGO1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly (PubMed:24554434). NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis (PubMed:25704143). Involved in the regulation of centrosome disjunction (PubMed:26220856). Phosphorylates CCDC102B either directly or indirectly which causes CCDC102B to dissociate from the centrosome and allows for centrosome separation (PubMed:30404835). Isoform 1. Phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2. Not present in the nucleolus and, in contrast to isoform 1, does not phosphorylate and activate NEK11 in G1/S-arrested cells.
Alternative names
NEK2A, NLK1, NEK2, Serine/threonine-protein kinase Nek2, HSPK 21, Never in mitosis A-related kinase 2, NimA-like protein kinase 1, NimA-related protein kinase 2
Recommended products
Rabbit Polyclonal NEK2 antibody. Suitable for IHC-P, ELISA, WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human NEK2 aa 250-300 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NEK2 aa 250-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P51955
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The cell cycle serine/threonine-protein kinase Nek2 also known as Never in Mitosis Gene A (NIMA)-related kinase 2 has a molecular weight of approximately 56 kDa. Expressed mainly in proliferating cells with high expression in the testes it primarily localizes to the centrosome and nucleus. This protein regulates centrosome separation and spindle formation. Such functions are essential for proper cell division contributing to the accurate segregation of chromosomes.
- Biological function summary
Nek2 involves key roles in cell cycle progression especially from the G2 to the M phase. It integrates into a complex regulatory network interacting with various cell division controllers. Nek2 phosphorylates centrosomal proteins which ensures timely centrosome disjunction and bipolar spindle formation. This action establishes Nek2 as important for the maintenance of genomic stability preventing abnormal cell divisions.
- Pathways
Nek2 fits into critical pathways that control cell division and mitosis. It operates within the PI3K/AKT pathway influencing cell growth and survival. Additionally Nek2 relates to other kinases like Aurora A kinase sharing pathway intersections that modulate centrosome dynamics and stability. These interactions reflect the significance of Nek2 in orchestrating precise mitotic events contributing to orderly cell cycle transitions.
- Associated diseases and disorders
Nek2 shows connections to cancers particularly breast and prostate cancer. Overexpression of Nek2 correlates with the pathogenesis of these cancers indicating its role in aberrant cellular proliferation. It also interacts with proteins like MAD2 which is a spindle checkpoint protein. This connection highlights Nek2's involvement in tumorigenesis through mitotic checkpoint control failure contributing to cancer cell survival and progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK2 antibody (ab115731)
ab115731 at 2.5ug/ml staining NEK2 in Human testis tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK2 antibody (ab115731)
ab115731 at 2.5ug/ml staining NEK2 in Human colon tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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