JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB133494

Anti-NEK6 antibody [EPR5283]

Be the first to review this product! Submit a review

|

(9 Publications)

Rabbit Recombinant Monoclonal NEK6 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 9 publications.

View Alternative Names

Serine/threonine-protein kinase Nek6, Never in mitosis A-related kinase 6, Protein kinase SID6-1512, NimA-related protein kinase 6, NEK6

2 Images
Western blot - Anti-NEK6 antibody [EPR5283] (AB133494)
  • WB

Unknown

Western blot - Anti-NEK6 antibody [EPR5283] (AB133494)

All lanes:

Western blot - Anti-NEK6 antibody [EPR5283] (ab133494) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

HT1080 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 36 kDa

false

Western blot - Anti-NEK6 antibody [EPR5283] (AB133494)
  • WB

Lab

Western blot - Anti-NEK6 antibody [EPR5283] (AB133494)

Lanes 1-3 : Merged signal (red and green). Green - ab133494 observed at 36 kDa. Red - loading control ab7291 observed at 50 kDa.

ab133494 Anti-NEK6 antibody [EPR5283] was shown to specifically react with NEK6 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265910 (knockout cell lysate ab258072) was used. Wild-type and NEK6 knockout samples were subjected to SDS-PAGE. ab133494 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NEK6 antibody [EPR5283] (ab133494) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

NEK6 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human NEK6 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-nek6-knockout-hela-cell-line-ab265910'>ab265910</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 36 kDa,53 kDa

Observed band size: 36 kDa,53 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5283

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein NIMA-related kinase 6 (NEK6) acts as a serine/threonine kinase involved in cell cycle regulation. It is also known as Never In Mitosis Gene A-related kinase 6. NEK6 has a molecular mass of approximately 40 kDa. Cells mainly express NEK6 in proliferating tissues. Its activity is dependent on phosphorylation by other kinases which allows NEK6 to proceed with mitosis by controlling different cellular processes.
Biological function summary

NEK6 plays a critical role in mitotic cell division by promoting progression through the G2/M phase of the cell cycle. Although NEK6 does not form part of a classical protein complex it does interact with various substrates to ensure successful mitosis. It stabilizes microtubule organization ensuring proper chromosomal segregation and cell cycle progression by facilitating microtubule nucleation and stability.

Pathways

NEK6 contributes to cell cycle control and microtubule dynamics being important within the context of the DNA damage response and repair pathways. NEK6 is associated with the p53 signaling pathway where it functions alongside related proteins such as NEK7. These kinases coordinate cellular responses to stress and damage ultimately maintaining genomic stability and preventing aberrant cell division.

NEK6 has been linked to oncogenesis especially in various types of cancer. Overexpression or dysregulation of NEK6 can promote tumor growth with studies showing its involvement in conditions such as breast cancer. NEK6 activity parallels oncogenic signals often interacting with proteins like p53 whose inactivation contributes to tumorigenesis. Understanding NEK6's role in these pathways may offer novel therapeutic targets for cancer treatment.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Protein kinase which plays an important role in mitotic cell cycle progression (PubMed : 11516946, PubMed : 14563848). Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis (PubMed : 19414596). Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3 (PubMed : 12054534, PubMed : 20873783). Phosphorylates KIF11 to promote mitotic spindle formation (PubMed : 19001501). Involved in G2/M phase cell cycle arrest induced by DNA damage (PubMed : 18728393). Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence (PubMed : 21099361). Phosphorylates EML4 at 'Ser-144', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed : 31409757).
See full target information Serine/threonine-protein kinase Nek6
style
left-column

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 4:101310 PubMed38118409

2023

Entrectinib inhibits NLRP3 inflammasome and inflammatory diseases by directly targeting NEK7.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyu Jin,Didi Liu,Xinru Zhou,Xianyu Luo,Qian Huang,Yi Huang

Glia 70:273-286 PubMed34643969

2021

NEK6 is an injury-responsive kinase cooperating with STAT3 in regulation of reactive astrogliosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Yu,Tianjin Shen,Xiaoling Zhong,Lei-Lei Wang,Wenjiao Tai,Yuhua Zou,Jun Qin,Zhaohui Zhang,Chun-Li Zhang

American journal of translational research 12:7528-7541 PubMed33312387

2020

microRNA-219-5p targets NEK6 to inhibit hepatocellular carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Gong Ting,Xin Li,Hyog Young Kwon,Tengteng Ding,Zhihao Zhang,Zhugui Chen,Chenglin Li,Youtan Liu,Yinggui Yang

Journal of diabetes investigation 12:334-345 PubMed32881354

2020

Micro-ribonucleic acid-23a-3p prevents the onset of type 2 diabetes mellitus by suppressing the activation of nucleotide-binding oligomerization-like receptor family pyrin domain containing 3 inflammatory bodies-caused pyroptosis through negatively regulating NIMA-related kinase 7.

Applications

Unspecified application

Species

Unspecified reactive species

Hongye Chang,Hongjuan Chang,Tuanjie Cheng,Garrick D Lee,Xiaoping Chen,Kunqing Qi

Nature cell biology 22:246-256 PubMed32015438

2020

LATS suppresses mTORC1 activity to directly coordinate Hippo and mTORC1 pathways in growth control.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjian Gan,Xiaoming Dai,Xiangpeng Dai,Jun Xie,Shasha Yin,Junjie Zhu,Chen Wang,Yuchen Liu,Jianping Guo,Min Wang,Jing Liu,Jia Hu,Ryan J Quinton,Neil J Ganem,Pengda Liu,John M Asara,Pier Paolo Pandolfi,Yingzi Yang,Zhigang He,Guangping Gao,Wenyi Wei

Nature communications 9:2330 PubMed29899413

2018

NEK7 regulates dendrite morphogenesis in neurons via Eg5-dependent microtubule stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Francisco Freixo,Paula Martinez Delgado,Yasmina Manso,Carlos Sánchez-Huertas,Cristina Lacasa,Eduardo Soriano,Joan Roig,Jens Lüders

Cancer research 77:753-765 PubMed27899381

2016

Castration Resistance in Prostate Cancer Is Mediated by the Kinase NEK6.

Applications

Unspecified application

Species

Unspecified reactive species

Atish D Choudhury,Anna C Schinzel,Maura B Cotter,Rosina T Lis,Katherine Labella,Ying Jie Lock,Francesca Izzo,Isil Guney,Michaela Bowden,Yvonne Y Li,Jinal Patel,Emily Hartman,Steven A Carr,Monica Schenone,Jacob D Jaffe,Philip W Kantoff,Peter S Hammerman,William C Hahn

Nature 530:354-7 PubMed26814970

2016

NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan He,Melody Y Zeng,Dahai Yang,Benny Motro,Gabriel Núñez

Nature immunology 17:250-8 PubMed26642356

2015

NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component.

Applications

WB

Species

Unspecified reactive species

Hexin Shi,Ying Wang,Xiaohong Li,Xiaoming Zhan,Miao Tang,Maggy Fina,Lijing Su,David Pratt,Chun Hui Bu,Sara Hildebrand,Stephen Lyon,Lindsay Scott,Jiexia Quan,Qihua Sun,Jamie Russell,Stephanie Arnett,Peter Jurek,Ding Chen,Vladimir V Kravchenko,John C Mathison,Eva Marie Y Moresco,Nancy L Monson,Richard J Ulevitch,Bruce Beutler
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB117986

Anti-NEK6 antibody [OTI2H7]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (AB117986)

  • 0
  • 0
Primary Antibodies

AB109177

Anti-NEK6 antibody [EPR5282]

RabMAb|Recombinant|KO Validated

Western blot - Anti-NEK6 antibody [EPR5282] (AB109177)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB133514

Anti-NEK7 antibody [EPR4900]

RabMAb|Recombinant|KO Validated|Trial Size|Trial Size

Western blot - Anti-NEK7 antibody [EPR4900] (AB133514)

  • 4
  • 2
Primary Antibodies

AB219800

Anti-GSDMD antibody [EPR20859]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-GSDMD antibody [EPR20859] (AB219800)

  • 5
  • 2
Primary Antibodies

AB76025

Anti-Casein Kinase 2 beta antibody [EP1995Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (AB76025)

  • 5
  • 6
Primary Antibodies

AB73349

Anti-Peroxiredoxin 3/PRDX3 antibody

BOND RX™ Validated|KO Validated

Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody (AB73349)

  • 4
  • 7

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com