AB117986

Anti-NEK6 antibody [OTI2H7]

Name: Anti-NEK6 antibody [OTI2H7] (ab117986) | Abcam
Brand: Abcam Limited
SKU: AB117986
Price: 495 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal NEK6 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Serine/threonine-protein kinase Nek6.

View Alternative Names

Serine/threonine-protein kinase Nek6, Never in mitosis A-related kinase 6, Protein kinase SID6-1512, NimA-related protein kinase 6, NEK6

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (AB117986)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (AB117986)

ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human ovary tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (AB117986)

ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human endometrium tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (AB117986)

ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (AB117986)

ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human breast adenocarcinoma tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (AB117986)

ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (AB117986)

ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human kidney carcinoma tissue by Immunohistochemistry.

Unknown

Western blot - Anti-NEK6 antibody [OTI2H7] (AB117986)

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203609)

All lanes:

Western blot - Anti-NEK6 antibody [OTI2H7] (ab117986) at 1/1000 dilution

Lane 1:

HEK293T cell lysate transfected with pCMV6-ENTRY control at 5 µg

Lane 2:

HEK293T cell lysate transfected with pCMV6-ENTRY NEK6 cDNA at 5 µg

Predicted band size: 36 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI2H7

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Serine/threonine-protein kinase Nek6.

Q9HC98

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" } } }

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Product details

Clone OTI2H7 (formerly 2H7).

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

ab117986 was purified from mouse ascites fluids by affinity chromatography.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein NIMA-related kinase 6 (NEK6) acts as a serine/threonine kinase involved in cell cycle regulation. It is also known as Never In Mitosis Gene A-related kinase 6. NEK6 has a molecular mass of approximately 40 kDa. Cells mainly express NEK6 in proliferating tissues. Its activity is dependent on phosphorylation by other kinases which allows NEK6 to proceed with mitosis by controlling different cellular processes.

Biological function summary

NEK6 plays a critical role in mitotic cell division by promoting progression through the G2/M phase of the cell cycle. Although NEK6 does not form part of a classical protein complex it does interact with various substrates to ensure successful mitosis. It stabilizes microtubule organization ensuring proper chromosomal segregation and cell cycle progression by facilitating microtubule nucleation and stability.

Pathways

NEK6 contributes to cell cycle control and microtubule dynamics being important within the context of the DNA damage response and repair pathways. NEK6 is associated with the p53 signaling pathway where it functions alongside related proteins such as NEK7. These kinases coordinate cellular responses to stress and damage ultimately maintaining genomic stability and preventing aberrant cell division.

NEK6 has been linked to oncogenesis especially in various types of cancer. Overexpression or dysregulation of NEK6 can promote tumor growth with studies showing its involvement in conditions such as breast cancer. NEK6 activity parallels oncogenic signals often interacting with proteins like p53 whose inactivation contributes to tumorigenesis. Understanding NEK6's role in these pathways may offer novel therapeutic targets for cancer treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein kinase which plays an important role in mitotic cell cycle progression (PubMed : 11516946, PubMed : 14563848). Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis (PubMed : 19414596). Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3 (PubMed : 12054534, PubMed : 20873783). Phosphorylates KIF11 to promote mitotic spindle formation (PubMed : 19001501). Involved in G2/M phase cell cycle arrest induced by DNA damage (PubMed : 18728393). Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence (PubMed : 21099361). Phosphorylates EML4 at 'Ser-144', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed : 31409757).

See full target information Serine/threonine-protein kinase Nek6

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in endocrinology 13:943686 PubMed35898455

2022

Predictive Role of NEK6 in Prognosis and Immune Infiltration in Head and Neck Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Min Yang,Bing Liao,Si-Si Yang,Tong Su,Jing Zhang,Wei-Ming Wang

Proceedings of the National Academy of Sciences of the United States of America 111:451-6 PubMed24335704

2013

Human herpesvirus 6 (HHV-6) alters E2F1/Rb pathways and utilizes the E2F1 transcription factor to express viral genes.

Applications

Unspecified application

Species

Unspecified reactive species

Eyal Sharon,Ludmila Volchek,Niza Frenkel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NEK6 antibody [OTI2H7]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (AB117986)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (AB117986)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (AB117986)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (AB117986)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (AB117986)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (AB117986)

Western blot - Anti-NEK6 antibody [OTI2H7] (AB117986)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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