Goat Polyclonal NEK7 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NEK7 aa 1-50.
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Publications
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- Frontiers in oncology 12:8126552022Inhibition of NEK7 Suppressed Hepatocellular Carcinoma Progression by Mediating Cancer Cell Pyroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZilong Yan,Qingen Da,Zhangfu Li,Qirui Lin,Jing Yi,Yanze Su,Guanyin Yu,Qingqi Ren,Xu Liu,Zewei Lin,Jianhua Qu,Weihua Yin,Jikui LiuPubMed 35223495
- Frontiers in oncology 11:7057972021NEK7 Promotes Pancreatic Cancer Progression And Its Expression Is Correlated With Poor Prognosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZilong Yan,Jianhua Qu,Zhangfu Li,Jing Yi,Yanze Su,Qirui Lin,Guangyin Yu,Zewei Lin,Weihua Yin,Fengmin Lu,Jikui LiuPubMed 34295827
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NEK7 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TDX7
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Bat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Common marmoset | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Elephant | Predicted | Predicted |
| Hamster | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Monkey | Predicted | Predicted |
| Opossum | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Turkey | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.05-0.2 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Horse, Chicken, Hamster, Cow, Dog, Turkey, Pig, Monkey, Opossum, Common marmoset, Bat, Elephant | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 3.75 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Horse, Chicken, Hamster, Cow, Dog, Turkey, Pig, Monkey, Opossum, Common marmoset, Bat, Elephant | Dilution info - | Notes - |
Associated Products
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Target data
Function
Protein kinase which plays an important role in mitotic cell cycle progression (PubMed:17101132, PubMed:19941817, PubMed:31409757). Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis (PubMed:17586473, PubMed:19414596, PubMed:19941817, PubMed:26522158, PubMed:31409757). Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed:31409757). Phosphorylates RPS6KB1 (By similarity). Acts as an essential activator of the NLRP3 inflammasome assembly independently of its kinase activity (PubMed:26642356, PubMed:36442502). Acts by unlocking NLRP3 following NLRP3 tranlocation into the microtubule organizing center (MTOC), relieving NLRP3 autoinhibition and promoting formation of the NLRP3:PYCARD complex, and activation of CASP1 (PubMed:26642356, PubMed:31189953, PubMed:36442502). Serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division: interaction with NEK9 prevents interaction with NLRP3 and activation of the inflammasome during mitosis (PubMed:26642356, PubMed:31189953).
Alternative names
Serine/threonine-protein kinase Nek7, Never in mitosis A-related kinase 7, NimA-related protein kinase 7, NEK7
Recommended products
Goat Polyclonal NEK7 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NEK7 aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NEK7 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TDX7
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The NIMA-related kinase 7 (NEK7) is a member of the NEK family of serine/threonine kinases and plays an important role in cell division. With a molecular mass of approximately 34 kDa the NEK7 protein is involved in mitosis by regulating microtubule organization. It is primarily expressed in various tissues including human and marmoset thyroid tissues. It remains mainly inactive until mitotic entry where its activity becomes essential for proper spindle formation and completion of mitosis.
- Biological function summary
Cell cycle progression heavily depends on NEK7's function. NEK7 interacts with NEDD1 in the microtubule-organizing center contributing to centrosome maturation and separation. This interaction makes NEK7 vital in the recruitment of γ-tubulin ring complex which is necessary for nucleating microtubules. NEK7 operates independently from the other NEK family members but shares involvement in centrosome-related activities.
- Pathways
NEK7 is influential in the regulation of the mitotic spindle assembly pathway and the interleukin-1 (IL-1) signaling pathway. It is closely related to proteins like NEK6 and NEK9 which also play roles in mitotic progression and cytokinesis. NEK7's involvement in these pathways indicates its integrative function in ensuring normal cell division and response to inflammatory signals.
- Associated diseases and disorders
NEK7 has implications in cancer and inflammatory ailments. NEK7 overexpression or mutation is often linked to tumorigenesis particularly in lung and brain cancers. Additionally NEK7 contributes to the activation of the NLRP3 inflammasome connecting it to inflammatory conditions like rheumatoid arthritis. The interplay with NLRP3 highlights the significance of NEK7 in inflammation-related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK7 antibody (ab166776)
Immunohistochemical analysis of formalin-fixed,
paraffin-embedded Human thyroid tissue labeling NEK7 with ab166776 at 3.75 µg/ml.
Western blot - Anti-NEK7 antibody (ab166776)
All lanes: Western blot - Anti-NEK7 antibody (ab166776) at 0.05 µg/mL
All lanes: HeLa lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 35 kDa
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