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Anti-NEK7 antibody [EPR4900] (ab133514) is a rabbit monoclonal antibody detecting NEK7 in Western Blot, IP. Suitable for Human, Mouse, Rat.



- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 60 publications


Images

Immunoprecipitation - Anti-NEK7 antibody [EPR4900] (AB133514), expandable thumbnail
  • Western blot - Anti-NEK7 antibody [EPR4900] (AB133514), expandable thumbnail
  • Western blot - Anti-NEK7 antibody [EPR4900] (AB133514), expandable thumbnail
  • Western blot - Anti-NEK7 antibody [EPR4900] (AB133514), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Expected
Tested
Not recommended
Mouse
Tested
Tested
Not recommended
Rat
Expected
Tested
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/10 - 1/100
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/10000
Notes

-

Species
Rat
Dilution info
1/10000
Notes

-

Species
Human
Dilution info
1/10000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Protein kinase which plays an important role in mitotic cell cycle progression (PubMed:17101132, PubMed:19941817, PubMed:31409757). Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis (PubMed:17586473, PubMed:19414596, PubMed:19941817, PubMed:26522158, PubMed:31409757). Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed:31409757). Phosphorylates RPS6KB1 (By similarity). Acts as an essential activator of the NLRP3 inflammasome assembly independently of its kinase activity (PubMed:26642356, PubMed:36442502). Acts by unlocking NLRP3 following NLRP3 tranlocation into the microtubule organizing center (MTOC), relieving NLRP3 autoinhibition and promoting formation of the NLRP3:PYCARD complex, and activation of CASP1 (PubMed:26642356, PubMed:31189953, PubMed:36442502). Serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division: interaction with NEK9 prevents interaction with NLRP3 and activation of the inflammasome during mitosis (PubMed:26642356, PubMed:31189953).

Alternative names

Serine/threonine-protein kinase Nek7, Never in mitosis A-related kinase 7, NimA-related protein kinase 7, NEK7

Recommended products

Anti-NEK7 antibody [EPR4900] (ab133514) is a rabbit monoclonal antibody detecting NEK7 in Western Blot, IP. Suitable for Human, Mouse, Rat.



- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 60 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4900
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-NEK7 antibody [EPR4900] (ab133514) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Human, Mouse, Rat samples.

What is the molecular weight of NEK7?


Anti-NEK7 [EPR4900] (ab133514) specifically detects a band for NEK7 (UniProt: Q8TDX7) at a molecular weight of 35kDa.

Trusted by the scientific community


Anti-NEK7 [EPR4900] (ab133514) was first used in a scientific publication in 2012 and has been cited over 60 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed


The specificity of Anti-NEK7 antibody [EPR4900] (ab133514) has been confirmed by Western blot testing in NEK7 Knockout HAP1 cells.



Other related products


We have a range of other formats of antibody clone [EPR4900] also available for your convenience:
ab133514, Carrier free - Anti-NEK7 antibody [EPR4900] - BSA and Azide free ab240065



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The NIMA-related kinase 7 (NEK7) is a member of the NEK family of serine/threonine kinases and plays an important role in cell division. With a molecular mass of approximately 34 kDa the NEK7 protein is involved in mitosis by regulating microtubule organization. It is primarily expressed in various tissues including human and marmoset thyroid tissues. It remains mainly inactive until mitotic entry where its activity becomes essential for proper spindle formation and completion of mitosis.

Biological function summary

Cell cycle progression heavily depends on NEK7's function. NEK7 interacts with NEDD1 in the microtubule-organizing center contributing to centrosome maturation and separation. This interaction makes NEK7 vital in the recruitment of γ-tubulin ring complex which is necessary for nucleating microtubules. NEK7 operates independently from the other NEK family members but shares involvement in centrosome-related activities.

Pathways

NEK7 is influential in the regulation of the mitotic spindle assembly pathway and the interleukin-1 (IL-1) signaling pathway. It is closely related to proteins like NEK6 and NEK9 which also play roles in mitotic progression and cytokinesis. NEK7's involvement in these pathways indicates its integrative function in ensuring normal cell division and response to inflammatory signals.

Associated diseases and disorders

NEK7 has implications in cancer and inflammatory ailments. NEK7 overexpression or mutation is often linked to tumorigenesis particularly in lung and brain cancers. Additionally NEK7 contributes to the activation of the NLRP3 inflammasome connecting it to inflammatory conditions like rheumatoid arthritis. The interplay with NLRP3 highlights the significance of NEK7 in inflammation-related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-NEK7 antibody [EPR4900] (ab133514), expandable thumbnail

    Immunoprecipitation - Anti-NEK7 antibody [EPR4900] (ab133514)

    ab133514 immunoprecipitating NEK7. 10μg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at a dilution of 1/1000.

    Lane 1: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10ug
    Lane 2: NIH/3T3 whole cell lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab133514 in NIH/3T3 whole cell lysate

    All lanes: Immunoprecipitation - Anti-NEK7 antibody [EPR4900] (ab133514)

    Predicted band size: 35 kDa

  • Western blot - Anti-NEK7 antibody [EPR4900] (ab133514), expandable thumbnail

    Western blot - Anti-NEK7 antibody [EPR4900] (ab133514)

    All lanes: Western blot - Anti-NEK7 antibody [EPR4900] (ab133514) at 1/10000 dilution

    Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

    Lane 2: C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 35 kDa

  • Western blot - Anti-NEK7 antibody [EPR4900] (ab133514), expandable thumbnail

    Western blot - Anti-NEK7 antibody [EPR4900] (ab133514)

    Lane 1: Wild-type HAP1 cell lysate (40 μg)
    Lane 2: NEK7 knockout HAP1 cell lysate (40 μg)
    Lane 3: A549 cell lysate (20 μg)
    Lane 4: Jurkat cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab133514 observed at 35 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab133514 was shown to specifically react with NEK7 when NEK7 knockout samples were used. Wild-type and NEK7 knockout samples were subjected to SDS-PAGE. ab133514 and ab18058 (loading control to Vinculin) were diluted at 1/10000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-NEK7 antibody [EPR4900] (ab133514)

    Predicted band size: 35 kDa

  • Western blot - Anti-NEK7 antibody [EPR4900] (ab133514), expandable thumbnail

    Western blot - Anti-NEK7 antibody [EPR4900] (ab133514)

    All lanes: Western blot - Anti-NEK7 antibody [EPR4900] (ab133514) at 1/10000 dilution

    Lane 1: Jurkat cell lysate at 10 µg

    Lane 2: A549 cell lysate at 10 µg

    Lane 3: C6 cell lysate at 10 µg

    Lane 4: RAW264.7 cell lysate at 10 µg

    Lane 5: PC12 cell lysate at 10 µg

    Lane 6: NIH/3T3 cell lysate at 10 µg

    Secondary

    All lanes: Goat-anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 35 kDa

    Observed band size: 32 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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