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Rabbit Recombinant Monoclonal NEK7 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human, Rat samples.

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Images

Immunoprecipitation - Anti-NEK7 antibody [EPR4900] - BSA and Azide free (AB240065), expandable thumbnail
  • Western blot - Anti-NEK7 antibody [EPR4900] - BSA and Azide free (AB240065), expandable thumbnail
  • Western blot - Anti-NEK7 antibody [EPR4900] - BSA and Azide free (AB240065), expandable thumbnail
  • Western blot - Anti-NEK7 antibody [EPR4900] - BSA and Azide free (AB240065), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Expected
Tested
Not recommended
Mouse
Tested
Tested
Not recommended
Rat
Expected
Tested
Not recommended

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Protein kinase which plays an important role in mitotic cell cycle progression (PubMed:17101132, PubMed:19941817, PubMed:31409757). Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis (PubMed:17586473, PubMed:19414596, PubMed:19941817, PubMed:26522158, PubMed:31409757). Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed:31409757). Phosphorylates RPS6KB1 (By similarity). Acts as an essential activator of the NLRP3 inflammasome assembly independently of its kinase activity (PubMed:26642356, PubMed:36442502). Acts by unlocking NLRP3 following NLRP3 tranlocation into the microtubule organizing center (MTOC), relieving NLRP3 autoinhibition and promoting formation of the NLRP3:PYCARD complex, and activation of CASP1 (PubMed:26642356, PubMed:31189953, PubMed:36442502). Serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division: interaction with NEK9 prevents interaction with NLRP3 and activation of the inflammasome during mitosis (PubMed:26642356, PubMed:31189953).

Alternative names

Serine/threonine-protein kinase Nek7, Never in mitosis A-related kinase 7, NimA-related protein kinase 7, NEK7

Recommended products

Rabbit Recombinant Monoclonal NEK7 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR4900
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab240065 is the carrier-free version of Anti-NEK7 antibody [EPR4900] ab133514.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The NIMA-related kinase 7 (NEK7) is a member of the NEK family of serine/threonine kinases and plays an important role in cell division. With a molecular mass of approximately 34 kDa the NEK7 protein is involved in mitosis by regulating microtubule organization. It is primarily expressed in various tissues including human and marmoset thyroid tissues. It remains mainly inactive until mitotic entry where its activity becomes essential for proper spindle formation and completion of mitosis.

Biological function summary

Cell cycle progression heavily depends on NEK7's function. NEK7 interacts with NEDD1 in the microtubule-organizing center contributing to centrosome maturation and separation. This interaction makes NEK7 vital in the recruitment of γ-tubulin ring complex which is necessary for nucleating microtubules. NEK7 operates independently from the other NEK family members but shares involvement in centrosome-related activities.

Pathways

NEK7 is influential in the regulation of the mitotic spindle assembly pathway and the interleukin-1 (IL-1) signaling pathway. It is closely related to proteins like NEK6 and NEK9 which also play roles in mitotic progression and cytokinesis. NEK7's involvement in these pathways indicates its integrative function in ensuring normal cell division and response to inflammatory signals.

Associated diseases and disorders

NEK7 has implications in cancer and inflammatory ailments. NEK7 overexpression or mutation is often linked to tumorigenesis particularly in lung and brain cancers. Additionally NEK7 contributes to the activation of the NLRP3 inflammasome connecting it to inflammatory conditions like rheumatoid arthritis. The interplay with NLRP3 highlights the significance of NEK7 in inflammation-related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-NEK7 antibody [EPR4900] - BSA and Azide free (ab240065), expandable thumbnail

    Immunoprecipitation - Anti-NEK7 antibody [EPR4900] - BSA and Azide free (ab240065)

    Anti-NEK7 antibody [EPR4900] ab133514 immunoprecipitating NEK7. 10μg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at a dilution of 1/1000.

    Lane 1: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10ug
    Lane 2: NIH/3T3 whole cell lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-NEK7 antibody [EPR4900] ab133514 in NIH/3T3 whole cell lysate

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NEK7 antibody [EPR4900] ab133514).

    All lanes: Immunoprecipitation - Anti-NEK7 antibody [EPR4900] (Anti-NEK7 antibody [EPR4900] ab133514)

    Predicted band size: 35 kDa

  • Western blot - Anti-NEK7 antibody [EPR4900] - BSA and Azide free (ab240065), expandable thumbnail

    Western blot - Anti-NEK7 antibody [EPR4900] - BSA and Azide free (ab240065)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NEK7 antibody [EPR4900] ab133514).

    All lanes: Western blot - Anti-NEK7 antibody [EPR4900] - BSA and Azide free (ab240065) at 1/10000 dilution

    Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

    Lane 2: C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 35 kDa

  • Western blot - Anti-NEK7 antibody [EPR4900] - BSA and Azide free (ab240065), expandable thumbnail

    Western blot - Anti-NEK7 antibody [EPR4900] - BSA and Azide free (ab240065)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NEK7 antibody [EPR4900] ab133514).

    Lane 1: Wild-type HAP1 cell lysate (40 μg)
    Lane 2: NEK7 knockout HAP1 cell lysate (40 μg)
    Lane 3: A549 cell lysate (20 μg)
    Lane 4: Jurkat cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - Anti-NEK7 antibody [EPR4900] ab133514 observed at 35 kDa. Red - loading control, ab18058, observed at 124 kDa.

    Anti-NEK7 antibody [EPR4900] ab133514 was shown to specifically react with NEK7 when NEK7 knockout samples were used. Wild-type and NEK7 knockout samples were subjected to SDS-PAGE. Anti-NEK7 antibody [EPR4900] ab133514 and ab18058 (loading control to Vinculin) were diluted at 1/10000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-NEK7 antibody [EPR4900] - BSA and Azide free (ab240065)

    Predicted band size: 35 kDa

  • Western blot - Anti-NEK7 antibody [EPR4900] - BSA and Azide free (ab240065), expandable thumbnail

    Western blot - Anti-NEK7 antibody [EPR4900] - BSA and Azide free (ab240065)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NEK7 antibody [EPR4900] ab133514).

    All lanes: Western blot - Anti-NEK7 antibody [EPR4900] - BSA and Azide free (ab240065) at 1/1000 dilution

    Lane 1: Jurkat cell lysate at 10 µg

    Lane 2: A549 cell lysate at 10 µg

    Lane 3: C6 cell lysate at 10 µg

    Lane 4: RAW264.7 cell lysate at 10 µg

    Lane 5: PC12 cell lysate at 10 µg

    Lane 6: NIH/3T3 cell lysate at 10 µg

    Secondary

    All lanes: Goat-anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 35 kDa

    Observed band size: 32 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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