JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB109433

Anti-NEK7 antibody [EPR4901]

Be the first to review this product! Submit a review

|

(2 Publications)

Knockout Tested Rabbit Recombinant Monoclonal NEK7 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Serine/threonine-protein kinase Nek7, Never in mitosis A-related kinase 7, NimA-related protein kinase 7, NEK7

3 Images
Flow Cytometry (Intracellular) - Anti-NEK7 antibody [EPR4901] (AB109433)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-NEK7 antibody [EPR4901] (AB109433)

Flow cytometry overlay histogram showing left wild-type HAP1 WT and right NEK7 knockout HAP1 WT stained with ab109433 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab109433) (1x 106 in 100μl at 0.2 μg/ml (1/3135 dilution)) for 30min at 22°C.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 dilution for 30min at 22°C

Isotype control antibody Recombinant Rabbit IgG, monoclonal [EPR25A] was used at the same concentration and conditions as the primary antibody (black line). Unlabelled sample was also used as a control (blue line).

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

PFA fixation is not recommended.

Western blot - Anti-NEK7 antibody [EPR4901] (AB109433)
  • WB

Unknown

Western blot - Anti-NEK7 antibody [EPR4901] (AB109433)

All lanes:

Western blot - Anti-NEK7 antibody [EPR4901] (ab109433) at 1/10000 dilution

Lane 1:

Human brain lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Lane 5:

Fetal liver lysate at 10 µg

Lane 6:

Jurkat cell lysate at 10 µg

Predicted band size: 35 kDa

false

Western blot - Anti-NEK7 antibody [EPR4901] (AB109433)
  • WB

Lab

Western blot - Anti-NEK7 antibody [EPR4901] (AB109433)

Lane 1 : Wild-type HAP1 cell lysate (40 μg)
Lane 2 : NEK7 knockout HAP1 cell lysate (40 μg)
Lane 3 : A549 cell lysate (20 μg)
Lane 4 : Jurkat cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab109433 observed at 35 kDa. Red - loading control, ab18058, observed at 124 kDa.

ab109433 was shown to specifically react with NEK7 when NEK7 knockout samples were used. Wild-type and NEK7 knockout samples were subjected to SDS-PAGE. ab109433 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NEK7 antibody [EPR4901] (ab109433)

Predicted band size: 35 kDa

false

  • Carrier free

    Anti-NEK7 antibody [EPR4901] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4901

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "0.2 µg/mL", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p><p>PFA fixation not recommended</p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NIMA-related kinase 7 (NEK7) is a member of the NEK family of serine/threonine kinases and plays an important role in cell division. With a molecular mass of approximately 34 kDa the NEK7 protein is involved in mitosis by regulating microtubule organization. It is primarily expressed in various tissues including human and marmoset thyroid tissues. It remains mainly inactive until mitotic entry where its activity becomes essential for proper spindle formation and completion of mitosis.
Biological function summary

Cell cycle progression heavily depends on NEK7's function. NEK7 interacts with NEDD1 in the microtubule-organizing center contributing to centrosome maturation and separation. This interaction makes NEK7 vital in the recruitment of γ-tubulin ring complex which is necessary for nucleating microtubules. NEK7 operates independently from the other NEK family members but shares involvement in centrosome-related activities.

Pathways

NEK7 is influential in the regulation of the mitotic spindle assembly pathway and the interleukin-1 (IL-1) signaling pathway. It is closely related to proteins like NEK6 and NEK9 which also play roles in mitotic progression and cytokinesis. NEK7's involvement in these pathways indicates its integrative function in ensuring normal cell division and response to inflammatory signals.

NEK7 has implications in cancer and inflammatory ailments. NEK7 overexpression or mutation is often linked to tumorigenesis particularly in lung and brain cancers. Additionally NEK7 contributes to the activation of the NLRP3 inflammasome connecting it to inflammatory conditions like rheumatoid arthritis. The interplay with NLRP3 highlights the significance of NEK7 in inflammation-related diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Protein kinase which plays an important role in mitotic cell cycle progression (PubMed : 17101132, PubMed : 19941817, PubMed : 31409757). Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis (PubMed : 17586473, PubMed : 19414596, PubMed : 19941817, PubMed : 26522158, PubMed : 31409757). Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed : 31409757). Phosphorylates RPS6KB1 (By similarity). Acts as an essential activator of the NLRP3 inflammasome assembly independently of its kinase activity (PubMed : 26642356, PubMed : 36442502). Acts by unlocking NLRP3 following NLRP3 tranlocation into the microtubule organizing center (MTOC), relieving NLRP3 autoinhibition and promoting formation of the NLRP3 : PYCARD complex, and activation of CASP1 (PubMed : 26642356, PubMed : 31189953, PubMed : 36442502). Serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division : interaction with NEK9 prevents interaction with NLRP3 and activation of the inflammasome during mitosis (PubMed : 26642356, PubMed : 31189953).
See full target information NEK7
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of clinical and experimental 11:4545-4551 PubMed31949852

2018

Characteristics of peripheral and intrahepatic regulatory B cells in HBV-related liver cirrhosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yali Xiong,Hongyan Wu,Yang Li,Rui Huang,Yong Liu,Yuxin Chen,Xiangan Zhao,Haiyan Chang,Jun Chen,Chao Wu

Scientific reports 8:8618 PubMed29872077

2018

MCC950, a specific small molecule inhibitor of NLRP3 inflammasome attenuates colonic inflammation in spontaneous colitis mice.

Applications

WB

Species

Mouse

Agampodi Promoda Perera,Ruchira Fernando,Tanvi Shinde,Rohit Gundamaraju,Benjamin Southam,Sukhwinder Singh Sohal,Avril A B Robertson,Kate Schroder,Dale Kunde,Rajaraman Eri
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB95873

Anti-NEK7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK7 antibody (AB95873)

  • 0
  • 0
Primary Antibodies

AB240065

Anti-NEK7 antibody [EPR4900] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-NEK7 antibody [EPR4900] - BSA and Azide free (AB240065)

  • 0
  • 0
Primary Antibodies

AB133514

Anti-NEK7 antibody [EPR4900]

RabMAb|Recombinant|KO Validated|Trial Size

Immunoprecipitation - Anti-NEK7 antibody [EPR4900] (AB133514)

  • 4
  • 2
Primary Antibodies

AB166776

Anti-NEK7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK7 antibody (AB166776)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB263899

Anti-NLRP3 antibody [EPR23094-1]

KO Validated|RabMAb|Recombinant|20ul selling size

Western blot - Anti-NLRP3 antibody [EPR23094-1] (AB263899)

  • 4
  • 2
Primary Antibodies

AB209845

Anti-GSDMD antibody [EPR19828]

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-GSDMD antibody [EPR19828] (AB209845)

  • 4
  • 3
Primary Antibodies

AB179515

Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (AB179515)

  • 3
  • 3
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com