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Rabbit Recombinant Monoclonal NEK7 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human NEK7 conjugated to Keyhole Limpet Haemocyanin.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK7 antibody [HL1348] - BSA and Azide free (AB313844), expandable thumbnail
  • Western blot - Anti-NEK7 antibody [HL1348] - BSA and Azide free (AB313844), expandable thumbnail
  • Western blot - Anti-NEK7 antibody [HL1348] - BSA and Azide free (AB313844), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NEK7 antibody [HL1348] - BSA and Azide free (AB313844), expandable thumbnail
  • Western blot - Anti-NEK7 antibody [HL1348] - BSA and Azide free (AB313844), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Synthetic Peptide within Human NEK7 conjugated to Keyhole Limpet Haemocyanin. Database link Q8TDX7

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Tested
Expected
Mouse
Expected
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/500 - 1/1000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Protein kinase which plays an important role in mitotic cell cycle progression (PubMed:17101132, PubMed:19941817, PubMed:31409757). Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis (PubMed:17586473, PubMed:19414596, PubMed:19941817, PubMed:26522158, PubMed:31409757). Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed:31409757). Phosphorylates RPS6KB1 (By similarity). Acts as an essential activator of the NLRP3 inflammasome assembly independently of its kinase activity (PubMed:26642356, PubMed:36442502). Acts by unlocking NLRP3 following NLRP3 tranlocation into the microtubule organizing center (MTOC), relieving NLRP3 autoinhibition and promoting formation of the NLRP3:PYCARD complex, and activation of CASP1 (PubMed:26642356, PubMed:31189953, PubMed:36442502). Serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division: interaction with NEK9 prevents interaction with NLRP3 and activation of the inflammasome during mitosis (PubMed:26642356, PubMed:31189953).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal NEK7 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human NEK7 conjugated to Keyhole Limpet Haemocyanin.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Synthetic Peptide within Human NEK7 conjugated to Keyhole Limpet Haemocyanin. Database link Q8TDX7
Carrier free

Yes

Clone number

HL1348

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The NIMA-related kinase 7 (NEK7) is a member of the NEK family of serine/threonine kinases and plays an important role in cell division. With a molecular mass of approximately 34 kDa the NEK7 protein is involved in mitosis by regulating microtubule organization. It is primarily expressed in various tissues including human and marmoset thyroid tissues. It remains mainly inactive until mitotic entry where its activity becomes essential for proper spindle formation and completion of mitosis.

Biological function summary

Cell cycle progression heavily depends on NEK7's function. NEK7 interacts with NEDD1 in the microtubule-organizing center contributing to centrosome maturation and separation. This interaction makes NEK7 vital in the recruitment of γ-tubulin ring complex which is necessary for nucleating microtubules. NEK7 operates independently from the other NEK family members but shares involvement in centrosome-related activities.

Pathways

NEK7 is influential in the regulation of the mitotic spindle assembly pathway and the interleukin-1 (IL-1) signaling pathway. It is closely related to proteins like NEK6 and NEK9 which also play roles in mitotic progression and cytokinesis. NEK7's involvement in these pathways indicates its integrative function in ensuring normal cell division and response to inflammatory signals.

Associated diseases and disorders

NEK7 has implications in cancer and inflammatory ailments. NEK7 overexpression or mutation is often linked to tumorigenesis particularly in lung and brain cancers. Additionally NEK7 contributes to the activation of the NLRP3 inflammasome connecting it to inflammatory conditions like rheumatoid arthritis. The interplay with NLRP3 highlights the significance of NEK7 in inflammation-related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK7 antibody [HL1348] - BSA and Azide free (ab313844), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK7 antibody [HL1348] - BSA and Azide free (ab313844)

    Paraffin-embedded mouse muscle tissue stained for NEK7 protein at cytoplasm with ab313844 at a 1/100 dilution in immunohistochemical analysis.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Western blot - Anti-NEK7 antibody [HL1348] - BSA and Azide free (ab313844), expandable thumbnail

    Western blot - Anti-NEK7 antibody [HL1348] - BSA and Azide free (ab313844)

    Samples were seperated by 12% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

    All lanes: Western blot - Anti-NEK7 antibody [HL1348] - BSA and Azide free (ab313844) at 1/1000 dilution

    Lane 1: Raji whole cell extracts at 30 µg

    Lane 2: NCI-H929 whole cell extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 34 kDa

  • Western blot - Anti-NEK7 antibody [HL1348] - BSA and Azide free (ab313844), expandable thumbnail

    Western blot - Anti-NEK7 antibody [HL1348] - BSA and Azide free (ab313844)

    All lanes: Western blot - Anti-NEK7 antibody [HL1348] - BSA and Azide free (ab313844) at 1/1000 dilution

    Lane 1: Raji (Human Burkitt's lymphoma cell line) whole cell extract at 30 µg

    Lane 2: NCI-H1299 (Human lung carcinoma cell line) whole cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Developed using the ECL technique.

    Predicted band size: 35 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NEK7 antibody [HL1348] - BSA and Azide free (ab313844), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NEK7 antibody [HL1348] - BSA and Azide free (ab313844)

    Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling NEK7 using ab313844 at a 1/500 dilution (green). Blue: Fluoroshield with DAPI. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1/1000.
    Cells were fixed in 4% paraformaldehyde at RT for 15 mins.

  • Western blot - Anti-NEK7 antibody [HL1348] - BSA and Azide free (ab313844), expandable thumbnail

    Western blot - Anti-NEK7 antibody [HL1348] - BSA and Azide free (ab313844)

    All lanes: Western blot - Anti-NEK7 antibody [HL1348] - BSA and Azide free (ab313844) at 1/1000 dilution

    Lane 1: Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

    Lane 2: NEK7 knockout (KO) HeLa cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Developed using the ECL technique.

    Predicted band size: 35 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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