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AB169406

Anti-NEK8 antibody

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(1 Publication)

Mouse Polyclonal NEK8 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NEK8.

View Alternative Names

JCK, NEK12A, NEK8, Serine/threonine-protein kinase Nek8, Never in mitosis A-related kinase 8, Nima-related protein kinase 12a, NimA-related protein kinase 8

1 Images
Western blot - Anti-NEK8 antibody (AB169406)
  • WB

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Western blot - Anti-NEK8 antibody (AB169406)

All lanes:

Western blot - Anti-NEK8 antibody (ab169406) at 1 µg/mL

Lane 1:

NEK8 transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Predicted band size: 75 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NEK8.

Q86SG6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NEK8 also known as NIMA-related kinase 8 is a serine/threonine kinase. It measures approximately 73 kDa. This kinase plays a role in cell cycle regulation and is predominantly expressed in the kidney liver and pancreas tissues. NEK8 localizes to centrosomes and cilia which suggests its involvement in the structure and function of these organelles.
Biological function summary

NEK8 functions in the regulation of cilia length and stability. It integrates within a signaling complex at the ciliary transition zone important for ciliary function. NEK8 interacts with other components such as inversin and polycystin-2 essential for maintaining normal ciliary processes and signal transduction. Its role in ciliary dynamics suggests its importance to the proper cellular communication and organ development.

Pathways

NEK8 participates in the cilia-related signaling pathways and cell cycle regulation. It influences the Hedgehog signaling pathway which is vital for various developmental processes. NEK8 also interacts with proteins like Gli family transcription factors within this pathway. Another pathway involving NEK8 is the DNA damage response where it collaborates with proteins like ATR to maintain genomic stability.

NEK8 is linked to nephronophthisis and polycystic kidney disease. Mutations within NEK8 impair normal ciliary functions leading to ciliopathies that affect kidney development and function. PKD1 and PKD2 proteins associated with polycystic kidney disease have correlated functional interactions with NEK8 highlighting its significance in maintaining renal health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia (PubMed : 37598857). Plays a role in organogenesis, and is involved in the regulation of the Hippo signaling pathway (PubMed : 26967905).
See full target information NEK8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 14:e1007489 PubMed29975683

2018

rbpms2 functions in Balbiani body architecture and ovary fate.

Applications

Unspecified application

Species

Unspecified reactive species

Odelya H Kaufman,KathyAnn Lee,Manon Martin,Sophie Rothhämel,Florence L Marlow
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Product promise

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