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AB63553

Anti-NEK9 (phospho T210) antibody

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(2 Publications)

Rabbit Polyclonal NEK9 phospho T210 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NEK9 phospho T210.

View Alternative Names

KIAA1995, NEK8, NERCC, NEK9, Serine/threonine-protein kinase Nek9, Nercc1 kinase, Never in mitosis A-related kinase 9, NimA-related kinase 8, NimA-related protein kinase 9, Nek8

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK9 (phospho T210) antibody (AB63553)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK9 (phospho T210) antibody (AB63553)

ab63553 staining Human colon. Staining is localised to the cytoplasm and nucleus.
Left panel : with primary antibody at 1 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Immunocytochemistry/ Immunofluorescence - Anti-NEK9 (phospho T210) antibody (AB63553)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NEK9 (phospho T210) antibody (AB63553)

ab63553 at 1/500 dilution staining HeLa cells, without (left) and with (right) immunizing peptide.

Western blot - Anti-NEK9 (phospho T210) antibody (AB63553)
  • WB

Unknown

Western blot - Anti-NEK9 (phospho T210) antibody (AB63553)

All lanes:

Western blot - Anti-NEK9 (phospho T210) antibody (ab63553) at 1/500 dilution

Lane 1:

extracts from HepG2 cells at 30 µg/mL

Lane 2:

extracts from HepG2 cells at 30 µg with immunizing peptide

Predicted band size: 107 kDa

Observed band size: 130 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human NEK9 phospho T210. The exact immunogen used to generate this antibody is proprietary information.

Q8TD19

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NEK9 also known as NIMA-related kinase 9 is a serine/threonine kinase with a molecular mass of approximately 118 kDa. It plays a role in the regulation of various cell cycle processes. NEK9 is active in both the cytoplasm and nucleus and expresses broadly in various tissues including the liver kidneys and pancreas. It also has homologs in other eukaryotic organisms indicating its conserved function across species.
Biological function summary

This protein modulates different aspects of mitosis particularly in spindle formation and chromosome segregation. NEK9 forms a part of a complex in conjunction with other kinases like NEK6 and NEK7 coordinating the early events of mitosis. Its activity supports centrosome separation and microtubule nucleation which are important for proper cell division.

Pathways

NEK9 is involved in the cell cycle and microtubule organization pathways. It works in conjunction with proteins such as PLK1 and Aurora A which regulate the entry and progression through mitosis. NEK9 activates NEK6 and NEK7 which further propagate signals necessary for mitotic spindle assembly ensuring efficient cell division.

NEK9 has links to cancer and neurological disorders. Abnormal NEK9 activity has associations with tumorigenesis particularly linked with the dysregulation of cell cycle checkpoints and malignant transformation. In neurological disorder contexts NEK9 interacts with proteins like tau perturbing normal cellular functions and potentially contributing to disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation (PubMed : 12101123, PubMed : 12840024, PubMed : 14660563, PubMed : 19941817). Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2 (PubMed : 11864968). Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues (PubMed : 11864968). Important for G1/S transition and S phase progression (PubMed : 12840024, PubMed : 14660563, PubMed : 19941817). Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively (PubMed : 12840024, PubMed : 14660563, PubMed : 19941817, PubMed : 26522158).
See full target information NEK9 phospho T210
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:342 PubMed36611072

2023

Overexpression of the NEK9-EG5 axis is a novel metastatic marker in pathologic stage T3 colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Meejeong Kim,Hui Jeong Jeong,Hyun-Min Ju,Ji-Young Song,Se Jin Jang,Jene Choi

American journal of human genetics 98:1030-1037 PubMed27153399

2016

Somatic Mutations in NEK9 Cause Nevus Comedonicus.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan L Levinsohn,Jeffrey L Sugarman,Jennifer M McNiff,Richard J Antaya,Keith A Choate
View all publications
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