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Rabbit Recombinant Monoclonal NELF antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Flow Cytometry (Intracellular) - Anti-NELF antibody [EPR8976(2)] (AB183022), expandable thumbnail
  • Western blot - Anti-NELF antibody [EPR8976(2)] (AB183022), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/300

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target data

Function

Couples NMDA-sensitive glutamate receptor signaling to the nucleus and triggers long-lasting changes in the cytoarchitecture of dendrites and spine synapse processes. Part of the cAMP response element-binding protein (CREB) shut-off signaling pathway. Stimulates outgrowth of olfactory axons and migration of gonadotropin-releasing hormone (GnRH) and luteinizing-hormone-releasing hormone (LHRH) neuronal cells.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal NELF antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR8976(2)

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry (Intracellular) - Anti-NELF antibody [EPR8976(2)] (ab183022), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-NELF antibody [EPR8976(2)] (ab183022)

    Intracellular Flow Cytometry analysis of PC3 cells labeling NELF using ab183022 at 1/300 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.

  • Western blot - Anti-NELF antibody [EPR8976(2)] (ab183022), expandable thumbnail

    Western blot - Anti-NELF antibody [EPR8976(2)] (ab183022)

    All lanes: Western blot - Anti-NELF antibody [EPR8976(2)] (ab183022) at 1/1000 dilution

    Lane 1: Human fetal liver lysate at 10 µg

    Lane 2: Human fetal kidney lysate at 10 µg

    Lane 3: Human colon cancer lysate at 10 µg

    Lane 4: Human testis lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 60 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com