AB183022

Anti-NELF antibody [EPR8976(2)]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal NELF antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

NELF, NSMF, NMDA receptor synaptonuclear signaling and neuronal migration factor, Nasal embryonic luteinizing hormone-releasing hormone factor, Nasal embryonic LHRH factor

2 Images

Flow Cytometry (Intracellular) - Anti-NELF antibody [EPR8976(2)] (AB183022)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NELF antibody [EPR8976(2)] (AB183022)

Intracellular Flow Cytometry analysis of PC3 cells labeling NELF using ab183022 at 1/300 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control : Rabbit monoclonal IgG.

Supplier Data

Western blot - Anti-NELF antibody [EPR8976(2)] (AB183022)

All lanes:

Western blot - Anti-NELF antibody [EPR8976(2)] (ab183022) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Lane 3:

Human colon cancer lysate at 10 µg

Lane 4:

Human testis lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 60 kDa

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Carrier free

Anti-NELF antibody [EPR8976(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8976(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/300", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NELF also known as Negative Elongation Factor consists of four subunits with an approximate molecular mass of 200 kDa. NELF is expressed in various tissues and plays a mechanical role in RNA polymerase II pausing during early transcription elongation. NELF temporarily halts the transcription process by binding the elongation complex affecting the progression of gene expression.

Biological function summary

The interaction of NELF with other initiation factors ensures proper RNA synthesis regulation. NELF forms a part of a complex with DSIF (DRB Sensitivity-Inducing Factor) and RNA polymerase II stabilizing the paused state of transcription. This regulatory action assists in controlling the timing of gene expression and allows other regulatory factors to modify transcription machinery or the chromatin environment.

Pathways

NELF participates in transcription regulation pathways including the c-myc pathway and the heat shock response. In the context of the c-myc pathway NELF works alongside proteins like P-TEFb which helps transition RNA polymerase II from the paused state to productive elongation. This cooperation ensures a responsive and tightly regulated transcription process.

Several cancers and cardiac diseases link to the improper regulation of NELF. Dysregulation of NELF and P-TEFb interactions correlates with aberrant transcription elongation control contributing to tumor development. Similarly disruptions in the heat shock response pathway can lead to cardiac disorders with HSF1 (Heat Shock Factor 1) interacting with NELF during stress response regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Couples NMDA-sensitive glutamate receptor signaling to the nucleus and triggers long-lasting changes in the cytoarchitecture of dendrites and spine synapse processes. Part of the cAMP response element-binding protein (CREB) shut-off signaling pathway. Stimulates outgrowth of olfactory axons and migration of gonadotropin-releasing hormone (GnRH) and luteinizing-hormone-releasing hormone (LHRH) neuronal cells.

See full target information NSMF

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 35:102363 PubMed39558906

2024

miRNA-199b-5p suppresses of oral squamous cell carcinoma by targeting apical-basolateral polarity via Scribble/Lgl.

Applications

Unspecified application

Species

Unspecified reactive species

Shihyun Kim,Suyeon Park,Yong-Jae Kim,Jeongeun Hyun,Jongho Choi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NELF antibody [EPR8976(2)]

Flow Cytometry (Intracellular) - Anti-NELF antibody [EPR8976(2)] (AB183022)

Western blot - Anti-NELF antibody [EPR8976(2)] (AB183022)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise