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AB250641

Anti-NELF antibody [EPR8976(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal NELF antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

NELF, NSMF, NMDA receptor synaptonuclear signaling and neuronal migration factor, Nasal embryonic luteinizing hormone-releasing hormone factor, Nasal embryonic LHRH factor

2 Images
Flow Cytometry (Intracellular) - Anti-NELF antibody [EPR8976(2)] - BSA and Azide free (AB250641)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NELF antibody [EPR8976(2)] - BSA and Azide free (AB250641)

This data was developed using ab183022, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of PC3 cells labeling NELF using ab183022 at 1/300 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control : Rabbit monoclonal IgG.

Western blot - Anti-NELF antibody [EPR8976(2)] - BSA and Azide free (AB250641)
  • WB

Supplier Data

Western blot - Anti-NELF antibody [EPR8976(2)] - BSA and Azide free (AB250641)

This data was developed using ab183022, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NELF antibody [EPR8976(2)] (<a href='/en-us/products/primary-antibodies/nelf-antibody-epr89762-ab183022'>ab183022</a>) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Lane 3:

Human colon cancer lysate at 10 µg

Lane 4:

Human testis lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 60 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8976(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Primary Antibodies

AB167401

Anti-NELF-B antibody [EPR11200]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELF-B antibody [EPR11200] (AB167401)

  • 5
  • 1
Primary Antibodies

AB85852

Anti-WHSC2/NELF-A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WHSC2/NELF-A antibody (AB85852)

  • 0
  • 0
Primary Antibodies

AB118418

Anti-INPP5A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP5A antibody (AB118418)

  • 0
  • 0
Primary Antibodies

AB154674

Anti-p38IP antibody

Western blot - Anti-p38IP antibody (AB154674)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab250641 is the carrier-free version of ab183022.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NELF also known as Negative Elongation Factor consists of four subunits with an approximate molecular mass of 200 kDa. NELF is expressed in various tissues and plays a mechanical role in RNA polymerase II pausing during early transcription elongation. NELF temporarily halts the transcription process by binding the elongation complex affecting the progression of gene expression.
Biological function summary

The interaction of NELF with other initiation factors ensures proper RNA synthesis regulation. NELF forms a part of a complex with DSIF (DRB Sensitivity-Inducing Factor) and RNA polymerase II stabilizing the paused state of transcription. This regulatory action assists in controlling the timing of gene expression and allows other regulatory factors to modify transcription machinery or the chromatin environment.

Pathways

NELF participates in transcription regulation pathways including the c-myc pathway and the heat shock response. In the context of the c-myc pathway NELF works alongside proteins like P-TEFb which helps transition RNA polymerase II from the paused state to productive elongation. This cooperation ensures a responsive and tightly regulated transcription process.

Several cancers and cardiac diseases link to the improper regulation of NELF. Dysregulation of NELF and P-TEFb interactions correlates with aberrant transcription elongation control contributing to tumor development. Similarly disruptions in the heat shock response pathway can lead to cardiac disorders with HSF1 (Heat Shock Factor 1) interacting with NELF during stress response regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Couples NMDA-sensitive glutamate receptor signaling to the nucleus and triggers long-lasting changes in the cytoarchitecture of dendrites and spine synapse processes. Part of the cAMP response element-binding protein (CREB) shut-off signaling pathway. Stimulates outgrowth of olfactory axons and migration of gonadotropin-releasing hormone (GnRH) and luteinizing-hormone-releasing hormone (LHRH) neuronal cells.
See full target information NSMF
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neuroreport 33:451-462 PubMed35775321

2022

Notoginsenoside R1 alleviates spinal cord injury by inhibiting oxidative stress, neuronal apoptosis, and inflammation via activating the nuclear factor erythroid 2 related factor 2/heme oxygenase-1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongbo Luo,Zhangli Bao,Mingjian Zhou,Yuxin Chen,Zhaoxi Huang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com