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AB232061

Anti-NELF-B antibody [EPR11200] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal NELF-B antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

COBRA1, KIAA1182, NELFB, Negative elongation factor B, NELF-B, Cofactor of BRCA1

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELF-B antibody [EPR11200] - BSA and Azide free (AB232061)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELF-B antibody [EPR11200] - BSA and Azide free (AB232061)

Formalin-fixed, paraffin-embedded human kidney tissue stained for NEFL-B with ab167401 (1/100 dilution) in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab167401).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELF-B antibody [EPR11200] - BSA and Azide free (AB232061)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELF-B antibody [EPR11200] - BSA and Azide free (AB232061)

Formalin-fixed, paraffin-embedded human colon tissue stained for NELF-B with ab167401 (1/100 dilution) in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab167401).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELF-B antibody [EPR11200] - BSA and Azide free (AB232061)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELF-B antibody [EPR11200] - BSA and Azide free (AB232061)

Formalin-fixed, paraffin-embedded human uterus tissue stained for NELF-B with ab167401 (1/100 dilution) in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab167401).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-NELF-B antibody [EPR11200] - BSA and Azide free (AB232061)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NELF-B antibody [EPR11200] - BSA and Azide free (AB232061)

Immunofluorescent analysis of BxPC-3 cells labeling NELF-B with ab167401 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab167401).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELF-B antibody [EPR11200] - BSA and Azide free (AB232061)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELF-B antibody [EPR11200] - BSA and Azide free (AB232061)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for NELF-B with ab167401 (1/100 dilution) in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab167401).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11200

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab232061 is the carrier-free version of ab167401.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NELF-B also known as Negative Elongation Factor B functions mechanically as part of the NELF complex that interacts with RNA polymerase II to pause transcription elongation. NELF-B's mass is about 66 kDa. This protein primarily expresses in the nucleus of cells particularly in tissue types where transcription regulation is essential like neurons and muscle cells which require precise gene expression control.
Biological function summary

NELF-B plays a role in regulating gene expression by pausing RNA polymerase II during early elongation. It operates as part of the larger NELF complex which also includes instigates the pausing mechanism. The NELF complex allows for the fine-tuning of transcription and is important for processes involving cell differentiation and response to external stimuli.

Pathways

NELF-B integrates within transcriptional regulation and RNA processing pathways. It directly affects the transcription cycle by contributing to the controlled release of RNA polymerase II from the paused state. NELF-B relates to proteins like DSIF (DRB Sensitivity-Inducing Factor) which collaborates with the NELF complex to modulate transcription dynamics and ensure proper gene expression regulation.

Aberrant function or expression of NELF-B links to cancer and cardiac hypertrophy. In cancer the dysregulation of transcriptional pausing by NELF-B can lead to unchecked cell proliferation. NELF-B also interacts with proteins like MYC where altered expression can amplify oncogenic pathways. In cardiac hypertrophy improper transcription regulation impacts heart muscle cell function emphasizing NELF-B's role in maintaining cellular homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (PubMed : 12612062). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (PubMed : 10199401). May be able to induce chromatin unfolding (PubMed : 11739404). Essential for early embryogenesis; plays an important role in maintaining the undifferentiated state of embryonic stem cells (ESCs) by preventing unscheduled expression of developmental genes (By similarity). Plays a key role in establishing the responsiveness of stem cells to developmental cues; facilitates plasticity and cell fate commitment in ESCs by establishing the appropriate expression level of signaling molecules (By similarity). Supports the transcription of genes involved in energy metabolism in cardiomyocytes; facilitates the association of transcription initiation factors with the promoters of the metabolism-related genes (By similarity).. (Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II (PubMed : 23884411). In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1 (PubMed : 23884411).
See full target information NELFB
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Translational lung cancer research 10:2523-2538 PubMed34295659

2021

One carbon metabolism in human lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sha Yao,Luogen Peng,Omar Elakad,Stefan Küffer,Marc Hinterthaner,Bernhard C Danner,Alexander von Hammerstein-Equord,Philipp Ströbel,Hanibal Bohnenberger
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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