Anti-NELFe antibody [EPR11600]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-NELFe antibody [EPR11600] (AB170104)

Immunofluorescence analysis of HepG2 cells labeling NELFe with ab170104 at a 1/100 dilution.

This image was generated using the unpurified version of the product.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-NELFe antibody [EPR11600] (AB170104)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling NELFe with Purified ab170104 at 1/300 dilution (1μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-NELFe antibody [EPR11600] (AB170104)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling NELFe with Purified ab170104 at 1 : 500 dilution (0.5 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IP

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Immunoprecipitation - Anti-NELFe antibody [EPR11600] (AB170104)

ab170104 (purified) at 1 : 20 dilution (2μg) immunoprecipitating NELFe in HeLa whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab170104 & HeLa whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab170104 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-NELFe antibody [EPR11600] (ab170104)

Predicted band size: 43 kDa

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• IP

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Immunoprecipitation - Anti-NELFe antibody [EPR11600] (AB170104)

Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (lane 1) or 1X PBS (negative control) (lane 2) using ab170104, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

This image was generated using the unpurified version of the product.

All lanes:

Immunoprecipitation - Anti-NELFe antibody [EPR11600] (ab170104)

Predicted band size: 43 kDa

false

• WB

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Western blot - Anti-NELFe antibody [EPR11600] (AB170104)

This image was generated using the unpurified version of the product.

All lanes:

Western blot - Anti-NELFe antibody [EPR11600] (ab170104) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

T47-D cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 43 kDa

false

• WB

Lab

Western blot - Anti-NELFe antibody [EPR11600] (AB170104)

All lanes:

Western blot - Anti-NELFe antibody [EPR11600] (ab170104) at 1/5000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

Mouse brain lysates at 20 µg

Lane 3:

Rat brain lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 44 kDa

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