AB181376

Anti-NELL2 antibody [EPR13539]

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal NELL2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

NRP2, NELL2, Protein kinase C-binding protein NELL2, NEL-like protein 2, Nel-related protein 2

2 Images

Supplier Data

Immunoprecipitation - Anti-NELL2 antibody [EPR13539] (AB181376)

Western blot analysis on immunoprecipitation pellet from fetal brain lysate using ab181376 at 1/10 dilution to immunoprecipitate NELL2.

All lanes:

Immunoprecipitation - Anti-NELL2 antibody [EPR13539] (ab181376)

Predicted band size: 91 kDa

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Supplier Data

Western blot - Anti-NELL2 antibody [EPR13539] (AB181376)

All lanes:

Western blot - Anti-NELL2 antibody [EPR13539] (ab181376) at 1/1000 dilution

Lane 1:

Human hypothalamus tissue lysate at 10 µg

Lane 2:

Human fetal brain tissue lysate at 10 µg

Predicted band size: 91 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13539

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NELL2 also known as NEL-like protein 2 is a secreted glycoprotein with a molecular mass approximately 92 kDa. This protein shows high expression in neural tissues particularly in the brain and spinal cord. NELL2 possesses six EGF-like domains and other motifs suggesting a role in cell-cell signaling. NELL2 actively functions in mediating neuronal differentiation and synaptic plasticity which are important for brain function.

Biological function summary

NELL2 plays a significant role in the development and regeneration of the nervous system. It does not appear to form part of a larger complex but interacts with various receptors on cell surfaces. NELL2 contributes to axon guidance promoting the survival and differentiation of specific neuron populations. Additionally NELL2 contributes to processes like learning and memory through its impact on synaptic formation and stability.

Pathways

NELL2 influences neuronal signaling pathways. It impacts the Nogo receptor pathway where it potentially competes or coordinates with proteins like Nogo and NgR1. This pathway is important for axonal outgrowth and regeneration. Furthermore NELL2 has involvement in pathways regulating synaptic activity where it interacts with other proteins that modulate neurotransmission affecting overall neural circuit dynamics.

NELL2 is linked to neurodegenerative conditions such as Alzheimer's disease and certain neurodevelopmental disorders. It shows altered expression in disease states implicating its role in synaptic dysfunction and neuronal loss. In Alzheimer's disease NELL2 may interact with amyloid precursor protein (APP) or tau proteins which are critical in disease pathology. Additionally its dysregulation can associate with autism spectrum disorders impacting neural connections linked to cognitive functions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays multiple roles in neural tissues, regulates neuronal proliferation, survival, differentiation, polarization, as well as axon guidance and synaptic functions. Plays an important role in axon development during neuronal differentiation through the MAPK intracellular signaling pathway (By similarity). Via binding to its receptor ROBO3, plays a role in axon guidance, functioning as a repulsive axon guidance cue that contributes to commissural axon guidance to the midline (PubMed : 32198364). Required for neuron survival through the modulation of MAPK signaling pathways too. Involved in the regulation of hypothalamic GNRH secretion and the control of puberty (By similarity).. Epididymal-secreted protein that signals through a ROS1-pathway to regulate the epididymal initial segment (IS) maturation, sperm maturation and male fertility.

See full target information NELL2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Genes & cancer 13:88-99 PubMed36533189

2022

Slit2 signaling stimulates Ewing sarcoma growth.

Applications

Unspecified application

Species

Unspecified reactive species

Kruthi Suvarna,Panneerselvam Jayabal,Xiuye Ma,Yuzuru Shiio

Brain pathology (Zurich, Switzerland) 33:e13131 PubMed36368713

2022

Early and extensive alterations of glial connexins, distal oligodendrogliopathy type demyelination, and nodal/paranodal pathology are characteristic of multiple system atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Yuji Nishimura,Katsuhisa Masaki,Dai Matsuse,Hiroo Yamaguchi,Tatsunori Tanaka,Eriko Matsuo,Shotaro Hayashida,Mitsuru Watanabe,Takuya Matsushita,Shoko Sadashima,Naokazu Sasagasako,Ryo Yamasaki,Noriko Isobe,Toru Iwaki,Jun-Ichi Kira

Genes & cancer 12:96-105 PubMed34966479

2021

EZH2 suppresses endogenous retroviruses and an interferon response in cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Panneerselvam Jayabal,Xiuye Ma,Yuzuru Shiio

Cell reports 36:109254 PubMed34233189

2021

NELL2-cdc42 signaling regulates BAF complexes and Ewing sarcoma cell growth.

Applications

Unspecified application

Species

Unspecified reactive species

Panneerselvam Jayabal,Fuchun Zhou,Xiufen Lei,Xiuye Ma,Barron Blackman,Susan T Weintraub,Peter J Houghton,Yuzuru Shiio

Frontiers in oncology 10:570916 PubMed33665159

2021

The tRNA-Derived Fragment-3017A Promotes Metastasis by Inhibiting NELL2 in Human Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Linhao Tong,Weixu Zhang,Bicheng Qu,Fei Zhang,Zhonghua Wu,Jinxin Shi,Xiaowan Chen,Yongxi Song,Zhenning Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NELL2 antibody [EPR13539]

Immunoprecipitation - Anti-NELL2 antibody [EPR13539] (AB181376)

Western blot - Anti-NELL2 antibody [EPR13539] (AB181376)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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