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AB250499

Anti-NELL2 antibody [EPR13539] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal NELL2 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

NRP2, NELL2, Protein kinase C-binding protein NELL2, NEL-like protein 2, Nel-related protein 2

2 Images
Immunoprecipitation - Anti-NELL2 antibody [EPR13539] - BSA and Azide free (AB250499)
  • IP

Supplier Data

Immunoprecipitation - Anti-NELL2 antibody [EPR13539] - BSA and Azide free (AB250499)

This data was developed using ab181376, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from fetal brain lysate using ab181376 at 1/10 dilution to immunoprecipitate NELL2.

All lanes:

Immunoprecipitation - Anti-NELL2 antibody [EPR13539] (<a href='/en-us/products/primary-antibodies/nell2-antibody-epr13539-ab181376'>ab181376</a>)

Predicted band size: 91 kDa

false

Western blot - Anti-NELL2 antibody [EPR13539] - BSA and Azide free (AB250499)
  • WB

Supplier Data

Western blot - Anti-NELL2 antibody [EPR13539] - BSA and Azide free (AB250499)

This data was developed using ab181376, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NELL2 antibody [EPR13539] (<a href='/en-us/products/primary-antibodies/nell2-antibody-epr13539-ab181376'>ab181376</a>) at 1/1000 dilution

Lane 1:

Human hypothalamus tissue lysate at 10 µg

Lane 2:

Human fetal brain tissue lysate at 10 µg

Predicted band size: 91 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13539

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab250499 is the carrier-free version of ab181376.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NELL2 also known as NEL-like protein 2 is a secreted glycoprotein with a molecular mass approximately 92 kDa. This protein shows high expression in neural tissues particularly in the brain and spinal cord. NELL2 possesses six EGF-like domains and other motifs suggesting a role in cell-cell signaling. NELL2 actively functions in mediating neuronal differentiation and synaptic plasticity which are important for brain function.
Biological function summary

NELL2 plays a significant role in the development and regeneration of the nervous system. It does not appear to form part of a larger complex but interacts with various receptors on cell surfaces. NELL2 contributes to axon guidance promoting the survival and differentiation of specific neuron populations. Additionally NELL2 contributes to processes like learning and memory through its impact on synaptic formation and stability.

Pathways

NELL2 influences neuronal signaling pathways. It impacts the Nogo receptor pathway where it potentially competes or coordinates with proteins like Nogo and NgR1. This pathway is important for axonal outgrowth and regeneration. Furthermore NELL2 has involvement in pathways regulating synaptic activity where it interacts with other proteins that modulate neurotransmission affecting overall neural circuit dynamics.

NELL2 is linked to neurodegenerative conditions such as Alzheimer's disease and certain neurodevelopmental disorders. It shows altered expression in disease states implicating its role in synaptic dysfunction and neuronal loss. In Alzheimer's disease NELL2 may interact with amyloid precursor protein (APP) or tau proteins which are critical in disease pathology. Additionally its dysregulation can associate with autism spectrum disorders impacting neural connections linked to cognitive functions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays multiple roles in neural tissues, regulates neuronal proliferation, survival, differentiation, polarization, as well as axon guidance and synaptic functions. Plays an important role in axon development during neuronal differentiation through the MAPK intracellular signaling pathway (By similarity). Via binding to its receptor ROBO3, plays a role in axon guidance, functioning as a repulsive axon guidance cue that contributes to commissural axon guidance to the midline (PubMed : 32198364). Required for neuron survival through the modulation of MAPK signaling pathways too. Involved in the regulation of hypothalamic GNRH secretion and the control of puberty (By similarity).. Epididymal-secreted protein that signals through a ROS1-pathway to regulate the epididymal initial segment (IS) maturation, sperm maturation and male fertility.
See full target information NELL2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:15564 PubMed38971897

2024

LILRB4 knockdown inhibits aortic dissection development by regulating pyroptosis and the JAK2/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianxian Xiong,Jiayuan Ling,Jie Yan,Yanyu Duan,Junjian Yu,Wentong Li,Wenbo Yu,Jianfeng Gao,Dilin Xie,Ziyou Liu,Yongzhi Deng,Yongling Liao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com