Rabbit Polyclonal NEMF antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human NEMF.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NEMF. The exact immunogen used to generate this antibody is proprietary information. Database link O60524
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/25.00000 - 1/100.00000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Target data
Function
Key component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates the extraction of incompletely synthesized nascent chains from stalled ribosomes as well as their ubiquitin-mediated proteasomal degradation (PubMed:25578875, PubMed:32726578, PubMed:33406423, PubMed:33909987). Thereby, frees 60S subunit ribosomes from the stalled translation complex and prevents the accumulation of nascent polypeptide chains that are potentially toxic for the cell (PubMed:25578875, PubMed:33406423, PubMed:33909987). Within the RQC complex, NEMF specifically binds stalled 60S ribosomal subunits by recognizing an exposed, nascent chain-conjugated tRNA moiety and promotes the recruitment of LTN1 to stalled 60S subunits (PubMed:25578875). Following binding to stalled 60S ribosomal subunits, NEMF mediates CAT tailing by recruiting alanine-charged tRNA to the A-site and directing the elongation of stalled nascent chains independently of mRNA or 40S subunits, leading to non-templated C-terminal alanine extensions (CAT tails) (PubMed:33406423, PubMed:33909987). Mainly recruits alanine-charged tRNAs, but can also other amino acid-charged tRNAs (PubMed:33406423, PubMed:33909987). CAT tailing is required to promote ubiquitination of stalled nascent chains by different E3 ubiquitin-protein ligases (PubMed:33909987). In the canonical RQC pathway (RQC-L), CAT tailing facilitates LTN1-dependent ubiquitination by exposing lysine residues that would otherwise remain buried in the ribosomal exit tunnel (By similarity). In the alternative RQC pathway (RQC-C) CAT tailing creates an C-degron mainly composed of alanine that is recognized by the CRL2(KLHDC10) and RCHY1/PIRH2 E3 ligases, leading to ubiquitination and degradation of stalled nascent chains (PubMed:33909987). NEMF may also indirectly play a role in nuclear export (PubMed:16103875).
Alternative names
SDCCAG1, NEMF, Ribosome quality control complex subunit NEMF, Antigen NY-CO-1, Nuclear export mediator factor, Serologically defined colon cancer antigen 1
Recommended products
Rabbit Polyclonal NEMF antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human NEMF.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NEMF. The exact immunogen used to generate this antibody is proprietary information. Database link O60524
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The NEMF protein also known as NEMF Shipping plays an important role in cellular processes. It functions as an important component of the ribosome-associated quality control complex facilitating the identification and degradation of faulty nascent peptides. NEMF has a molecular mass of approximately 110 kDa and is mostly expressed in the cytoplasm of eukaryotic cells. This protein operates with other factors to maintain the integrity of protein synthesis and prevent the accumulation of malfunctioning proteins within the cell.
- Biological function summary
NEMF is part of the ribosome-associated quality control complex that monitors and ensures proper translation and protein folding. This complex attaches to the ribosome during translation stalling events marking incomplete or misfolded polypeptides for ubiquitination and subsequent degradation by the proteasome. By participating in this surveillance mechanism NEMF supports protein homeostasis which is important for normal cellular function. It often collaborates with other components like LTN1 an E3 ubiquitin ligase and RQC2/NEM2 aiding efficient processing of aberrant proteins.
- Pathways
The NEMF protein has substantial importance in the cellular quality control pathways. It engages intimately with the ribosome-associated quality control pathway which rescues stalled ribosomes and targets them for recycling. Additionally NEMF is involved in pathways that regulate protein synthesis at times of cellular stress. Within these frameworks NEMF partners with proteins like RPL26 and ASCC3 which work to maintain translational fidelity and proteostasis.
- Associated diseases and disorders
NEMF has been implicated in neurodegenerative diseases and cancer. Abnormalities in the quality control mechanisms it safeguards can lead to the accumulation of defective proteins contributing to conditions such as amyotrophic lateral sclerosis (ALS). Furthermore altered NEMF function or expression has been linked to tumorigenesis as proper protein synthesis and degradation play significant roles in cancer progression. NEMF works alongside other proteins like p53 which is often mutated in cancer highlighting its involvement in pathways important for cellular health and disease prevention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-NEMF antibody (ab200382)
6% SDS-PAGE.
All lanes: Western blot - Anti-NEMF antibody (ab200382) at 1/200 dilution
All lanes: Jurkat cell lysate at 40 µg
Predicted band size: 123 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEMF antibody (ab200382)
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling NEMF with ab200382 at 1/30 dilution.
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