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AB74474

Anti-NENF antibody

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(2 Publications)

Rabbit Polyclonal NENF antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NENF.

View Alternative Names

CIR2, SPUF, NENF, Neudesin, Cell immortalization-related protein 2, Neuron-derived neurotrophic factor, Protein GIG47, Secreted protein of unknown function, SPUF protein

1 Images
Western blot - Anti-NENF antibody (AB74474)
  • WB

Unknown

Western blot - Anti-NENF antibody (AB74474)

Lane 1:

Western blot - Anti-NENF antibody (ab74474) at 1 µg/mL

Lane 2:

Western blot - Anti-NENF antibody (ab74474) at 2 µg/mL

All lanes:

Human kidney tissue lysate at 15 µg

Predicted band size: 19 kDa

Observed band size: 37 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human NENF. The exact immunogen used to generate this antibody is proprietary information.

Q9UMX5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein called Neuron-Derived Neurotrophic Factor (NENF) also known as BEND5 plays important roles in cellular processes. This protein weighs approximately 18 kDa. NENF is predominantly found in the central nervous system including the brain and spinal cord where it influences neural function. The protein is secreted and operates primarily in extracellular spaces where it interacts with receptors to execute its functions.
Biological function summary

The protein NENF influences cell survival and differentiation. It acts as a neurotrophic factor contributing to the development and maintenance of neurons. Although not part of a larger complex NENF interacts directly with receptors on target cells to initiate signaling cascades. This modulating effect on neural cells plays a substantial role in maintaining cellular mechanisms such as synaptic plasticity and neural circuit formation.

Pathways

NENF actively engages in the neurotrophic signaling pathway which is essential for proper nervous system function. It interacts with proteins such as the tyrosine receptor kinase (Trk) family influencing downstream effects on neuron maintenance and plasticity. In addition NENF takes part in the MAPK (Mitogen-Activated Protein Kinase) pathway contributing to cell growth and survival via its neurotrophic attributes highlighting its importance in complex neurological signaling processes.

NENF shows significant correlations with neurodegenerative conditions like Alzheimer's disease. In Alzheimer's disrupted NENF activity may influence neuron survival aggravating disease progression. Also studies suggest potential connections to Parkinson's disease with changes in NENF potentially linked to protein aggregates that harm nerve cells. In these diseases its interaction with the protein α-synuclein seems relevant with these interactions likely affecting disease development through neurodegeneration-related pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival (PubMed : 31536960). Promotes cell proliferation and neurogenesis in undifferentiated neural progenitor cells at the embryonic stage and inhibits differentiation of astrocytes (By similarity). Its neurotrophic activity is exerted via MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT pathways (By similarity). Neurotrophic activity is enhanced by binding to heme (By similarity). Also acts as an anorexigenic neurotrophic factor that contributes to energy balance (By similarity).
See full target information NENF
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:579951 PubMed33738280

2021

MANF Produced by MRL Mouse-Derived Mesenchymal Stem Cells Is Pro-regenerative and Protects From Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Gautier Tejedor,Patricia Luz-Crawford,Audrey Barthelaix,Karine Toupet,Sébastien Roudières,François Autelitano,Christian Jorgensen,Farida Djouad

Clinical cancer research : an official journal of the American Association for Cancer Research 20:3118-32 PubMed24763612

2014

Genome-wide study of hypomethylated and induced genes in patients with liver cancer unravels novel anticancer targets.

Applications

WB

Species

Human

Barbara Stefanska,David Cheishvili,Matthew Suderman,Ani Arakelian,Jian Huang,Michael Hallett,Ze-Guang Han,Mamun Al-Mahtab,Sheikh Mohammad Fazle Akbar,Wasif Ali Khan,Rubhana Raqib,Imrana Tanvir,Haseeb Ahmed Khan,Shafaat A Rabbani,Moshe Szyf
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Product promise

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