AB244408

Anti-Neogenin antibody

Name: Anti-Neogenin antibody (ab244408) | Abcam
Brand: Abcam Limited
SKU: AB244408
Price: 630 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Neogenin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NEO1 aa 1100 to C-terminus.

View Alternative Names

IGDCC2, NGN, NEO1, Neogenin, Immunoglobulin superfamily DCC subclass member 2

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neogenin antibody (AB244408)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neogenin antibody (AB244408)

Paraffin-embedded human prostate tissue stained for Neogenin using ab244408 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neogenin antibody (AB244408)

Paraffin-embedded human kidney tissue stained for Neogenin using ab244408 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neogenin antibody (AB244408)

Paraffin-embedded human colon tissue stained for Neogenin using ab244408 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Neogenin antibody (AB244408)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Neogenin antibody (AB244408)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Neogenin (green) using ab244408 at 4 μg/ml in ICC/IF.

Supplier Data

Western blot - Anti-Neogenin antibody (AB244408)

All lanes:

Western blot - Anti-Neogenin antibody (ab244408) at 0.4 µg/mL

Lane 1:

A549 (Human lung carcinoma cell line) cell lysate

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

Predicted band size: 160 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NEO1 aa 1100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q92859

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neogenin also known as NEO1 acts as an important receptor involved in cell adhesion migration and neural development. It weighs approximately 186 kDa. Neogenin binds to netrins and repulsive guidance molecules (RGMs) playing a role in the organization of neural circuits. Expressed in tissues such as the developing nervous system muscle tissue and some cancer cells Neogenin collaborates with cellular structures to influence growth and directionality.

Biological function summary

Neogenin influences cell orientation and axon guidance. It forms part of receptor complexes that include proteins like RGM and attracts molecules involved in neural signal transmission. Through these complexes Neogenin impacts processes such as synaptic development and neuronal positioning. Its activity allows for proper nervous system formation and function contributing to precise cellular communication.

Pathways

Neogenin participates in the signaling pathways important for neural pattern formation and tissue structuring. The Netrin signaling pathway is important for neuron navigation where Neogenin interacts with proteins like DCC (Deleted in Colorectal Cancer). Neogenin also engages in the Bone Morphogenetic Protein (BMP) pathways regulating cellular differentiation and tissue architecture.

Neogenin mutations or irregular expressions often associate with neurological disorders and cancer. In particular its dysregulation links to conditions such as brain tumors and congenital brain malformations. In cancer pathways Neogenin connects with proteins like Netrin-1 influencing pathways that lead to tumorigenesis and tumor progression. Understanding Neogenin interactions can help in developing therapeutic strategies targeting these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Multi-functional cell surface receptor regulating cell adhesion in many diverse developmental processes, including neural tube and mammary gland formation, myogenesis and angiogenesis. Receptor for members of the BMP, netrin, and repulsive guidance molecule (RGM) families. Netrin-Neogenin interactions result in a chemoattractive axon guidance response and cell-cell adhesion, the interaction between NEO1/Neogenin and RGMa and RGMb induces a chemorepulsive response.

See full target information NEO1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Extracellular vesicles and circulating nucleic acids 5:397-416 PubMed39697634

2024

Transfer of and increases 3D growth and invasiveness in recipient cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah M Nelson,Shimian Qu,Liyu Huang,Muhammad Shameer,Kevin C Corn,Sydney N Chapman,Nicole L Luthcke,Sara A Schuster,Tellie D Stamaris,Lauren A Turnbull,Lucas L Guy,Xiao Liu,Danielle L Michell,Elizabeth M Semler,Kasey C Vickers,Qi Liu,Jeffrey L Franklin,Alissa M Weaver,Marjan Rafat,Robert J Coffey,James G Patton

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Neogenin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neogenin antibody (AB244408)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neogenin antibody (AB244408)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neogenin antibody (AB244408)

Immunocytochemistry/ Immunofluorescence - Anti-Neogenin antibody (AB244408)

Western blot - Anti-Neogenin antibody (AB244408)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise