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AB183511

Anti-Neogenin antibody [EPR14696]

5

(1 Review)

|

(5 Publications)

Knockout Tested Rabbit Recombinant Monoclonal Neogenin antibody. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

IGDCC2, NGN, NEO1, Neogenin, Immunoglobulin superfamily DCC subclass member 2

4 Images
Immunoprecipitation - Anti-Neogenin antibody [EPR14696] (AB183511)
  • IP

Supplier Data

Immunoprecipitation - Anti-Neogenin antibody [EPR14696] (AB183511)

ab183511 at 1/50 immunoprecipitatinging Neogenin in human fetal brain cell lysate. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary.

All lanes:

Immunoprecipitation - Anti-Neogenin antibody [EPR14696] (ab183511)

Predicted band size: 160 kDa

Observed band size: 160 kDa

false

Western blot - Anti-Neogenin antibody [EPR14696] (AB183511)
  • WB

Lab

Western blot - Anti-Neogenin antibody [EPR14696] (AB183511)

Western blot : Anti-NEO1 antibody [EPR14696] (ab183511) staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab183511 was shown to bind specifically to NEO1. A band was observed at 130-200 kDa in wild-type A549 cell lysates with no signal observed at this size in NEO1 knockout cell line ab282825 (knockout cell lysate ab283049). To generate this image, wild-type and NEO1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Neogenin antibody [EPR14696] (ab183511) at 1/500 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

NEO1 knockout A549 cell lysate at 20 µg

Lane 3:

SH-SY5Y cell lysate at 20 µg

Lane 4:

Neuro-2a cell lysate at 20 µg

Observed band size: 130-200 kDa

true

Western blot - Anti-Neogenin antibody [EPR14696] (AB183511)
  • WB

Supplier Data

Western blot - Anti-Neogenin antibody [EPR14696] (AB183511)

All lanes:

Western blot - Anti-Neogenin antibody [EPR14696] (ab183511) at 1/50000 dilution

All lanes:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 160 kDa

Observed band size: 160 kDa

false

Western blot - Anti-Neogenin antibody [EPR14696] (AB183511)
  • WB

Supplier Data

Western blot - Anti-Neogenin antibody [EPR14696] (AB183511)

All lanes:

Western blot - Anti-Neogenin antibody [EPR14696] (ab183511) at 1/5000 dilution

All lanes:

A549 (Human lung carcinoma cell line) lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 160 kDa

Observed band size: 160 kDa

false

  • Carrier free

    Anti-Neogenin antibody [EPR14696] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14696

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50 - 1/70", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neogenin also known as NEO1 acts as an important receptor involved in cell adhesion migration and neural development. It weighs approximately 186 kDa. Neogenin binds to netrins and repulsive guidance molecules (RGMs) playing a role in the organization of neural circuits. Expressed in tissues such as the developing nervous system muscle tissue and some cancer cells Neogenin collaborates with cellular structures to influence growth and directionality.
Biological function summary

Neogenin influences cell orientation and axon guidance. It forms part of receptor complexes that include proteins like RGM and attracts molecules involved in neural signal transmission. Through these complexes Neogenin impacts processes such as synaptic development and neuronal positioning. Its activity allows for proper nervous system formation and function contributing to precise cellular communication.

Pathways

Neogenin participates in the signaling pathways important for neural pattern formation and tissue structuring. The Netrin signaling pathway is important for neuron navigation where Neogenin interacts with proteins like DCC (Deleted in Colorectal Cancer). Neogenin also engages in the Bone Morphogenetic Protein (BMP) pathways regulating cellular differentiation and tissue architecture.

Neogenin mutations or irregular expressions often associate with neurological disorders and cancer. In particular its dysregulation links to conditions such as brain tumors and congenital brain malformations. In cancer pathways Neogenin connects with proteins like Netrin-1 influencing pathways that lead to tumorigenesis and tumor progression. Understanding Neogenin interactions can help in developing therapeutic strategies targeting these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multi-functional cell surface receptor regulating cell adhesion in many diverse developmental processes, including neural tube and mammary gland formation, myogenesis and angiogenesis. Receptor for members of the BMP, netrin, and repulsive guidance molecule (RGM) families. Netrin-Neogenin interactions result in a chemoattractive axon guidance response and cell-cell adhesion, the interaction between NEO1/Neogenin and RGMa and RGMb induces a chemorepulsive response.
See full target information NEO1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 21:e1013338 PubMed40623098

2025

Proximity labelling of internalizing influenza A viruses reveals a role for neogenin in virus uptake.

Applications

Unspecified application

Species

Unspecified reactive species

Milagros Sempere Borau,Victor G Gisbert,Josephine von Kempis,Laura M Arroyo-Fernández,Samira Schiefer,David Alsteens,Silke Stertz

Cell communication and signaling : CCS 20:122 PubMed35974411

2022

Netrin-1 induces the anti-apoptotic and pro-survival effects of B-ALL cells through the Unc5b-MAPK axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lan Huang,Xizhou An,Yao Zhu,Kainan Zhang,Li Xiao,Xinyuan Yao,Xing Zeng,Shaoyan Liang,Jie Yu

Frontiers in cell and developmental biology 10:803029 PubMed35186922

2022

Upregulation of Neogenin-1 by a CREB1-BAF47 Complex in Vascular Endothelial Cells is Implicated in Atherogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Li,Hong Liu,Yujia Xue,Junliang Chen,Xiaocen Kong,Yuanyuan Zhang

Cancer cell international 20:510 PubMed33088218

2020

Identification of NEO1 as a prognostic biomarker and its effects on the progression of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Zhang,Zhou Zhou,Xue-Kai Pan,Yun-Jiao Zhou,Hai-Ou Li,Pei-Shan Qiu,Meng-Na Zhang,Ru-Yi Peng,Hai-Zhou Wang,Lan Liu,Jing Liu,Qiu Zhao

Cellular physiology and biochemistry : internation 48:1457-1467 PubMed30064133

2018

Neogenin-1 Promotes Cell Proliferation, Motility, and Adhesion by Up-Regulation of Zinc Finger E-Box Binding Homeobox 1 Via Activating the Rac1/PI3K/AKT Pathway in Gastric Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hengyi Qu,Huabo Sun,Xueping Wang
View all publications
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Product promise

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