Anti-Neogenin antibody [EPR14696] - BSA and Azide free

• WB

Lab

Western blot - Anti-Neogenin antibody [EPR14696] - BSA and Azide free (AB215521)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183511). Western blot : Anti-NEO1 antibody [EPR14696] (ab183511) staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab183511 was shown to bind specifically to NEO1. A band was observed at 130-200 kDa in wild-type A549 cell lysates with no signal observed at this size in NEO1 knockout cell line ab282825 (knockout cell lysate ab283049). To generate this image, wild-type and NEO1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Neogenin antibody [EPR14696] (<a href='/en-us/products/primary-antibodies/neogenin-antibody-epr14696-ab183511'>ab183511</a>) at 1/500 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

NEO1 knockout A549 cell lysate at 20 µg

Lane 3:

SH-SY5Y cell lysate at 20 µg

Lane 4:

Neuro-2a cell lysate at 20 µg

Observed band size: 130-200 kDa

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• IP

Supplier Data

Immunoprecipitation - Anti-Neogenin antibody [EPR14696] - BSA and Azide free (AB215521)

ab183511 at 1/50 immunoprecipitatinging Neogenin in human fetal brain cell lysate. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183511).

All lanes:

Immunoprecipitation - Anti-Neogenin antibody [EPR14696] (<a href='/en-us/products/primary-antibodies/neogenin-antibody-epr14696-ab183511'>ab183511</a>)

Predicted band size: 160 kDa

Observed band size: 160 kDa

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