Skip to main content

Knockout Tested Rabbit Recombinant Monoclonal Neogenin antibody. Suitable for WB, ICC/IF, IP and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-Neogenin antibody [EPR25220-56] (AB302542), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Neogenin antibody [EPR25220-56] (AB302542), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Neogenin antibody [EPR25220-56] (AB302542), expandable thumbnail
  • Western blot - Anti-Neogenin antibody [EPR25220-56] (AB302542), expandable thumbnail
  • Western blot - Anti-Neogenin antibody [EPR25220-56] (AB302542), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFIPFlow Cyt
Human
Tested
Not recommended
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Multi-functional cell surface receptor regulating cell adhesion in many diverse developmental processes, including neural tube and mammary gland formation, myogenesis and angiogenesis. Receptor for members of the BMP, netrin, and repulsive guidance molecule (RGM) families. Netrin-Neogenin interactions result in a chemoattractive axon guidance response and cell-cell adhesion, the interaction between NEO1/Neogenin and RGMa and RGMb induces a chemorepulsive response.

Alternative names

IGDCC2, NGN, NEO1, Neogenin, Immunoglobulin superfamily DCC subclass member 2

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal Neogenin antibody. Suitable for WB, ICC/IF, IP and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR25220-56
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Neogenin also known as NEO1 acts as an important receptor involved in cell adhesion migration and neural development. It weighs approximately 186 kDa. Neogenin binds to netrins and repulsive guidance molecules (RGMs) playing a role in the organization of neural circuits. Expressed in tissues such as the developing nervous system muscle tissue and some cancer cells Neogenin collaborates with cellular structures to influence growth and directionality.

Biological function summary

Neogenin influences cell orientation and axon guidance. It forms part of receptor complexes that include proteins like RGM and attracts molecules involved in neural signal transmission. Through these complexes Neogenin impacts processes such as synaptic development and neuronal positioning. Its activity allows for proper nervous system formation and function contributing to precise cellular communication.

Pathways

Neogenin participates in the signaling pathways important for neural pattern formation and tissue structuring. The Netrin signaling pathway is important for neuron navigation where Neogenin interacts with proteins like DCC (Deleted in Colorectal Cancer). Neogenin also engages in the Bone Morphogenetic Protein (BMP) pathways regulating cellular differentiation and tissue architecture.

Associated diseases and disorders

Neogenin mutations or irregular expressions often associate with neurological disorders and cancer. In particular its dysregulation links to conditions such as brain tumors and congenital brain malformations. In cancer pathways Neogenin connects with proteins like Netrin-1 influencing pathways that lead to tumorigenesis and tumor progression. Understanding Neogenin interactions can help in developing therapeutic strategies targeting these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-Neogenin antibody [EPR25220-56] (ab302542), expandable thumbnail

    Western blot - Anti-Neogenin antibody [EPR25220-56] (ab302542)

    Western blot: Anti-NEO1 antibody [EPR25220-56] (ab302542) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab302542 was shown to bind specifically to NEO1. A band was observed at 110-200 kDa in wild-type A549 cell lysates with no signal observed at this size in NEO1 knockout cell line ab282825 (knockout cell lysate ab283049). To generate this image, wild-type and NEO1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    All lanes: Western blot - Anti-Neogenin antibody [EPR25220-56] (ab302542)

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: NEO1 knockout A549 cell lysate at 20 µg

    Lane 3: SH-SY5Y cell lysate at 20 µg

    Lane 4: Western blot at 20 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 110-200 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Neogenin antibody [EPR25220-56] (ab302542), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Neogenin antibody [EPR25220-56] (ab302542)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labelling Neogenin with ab302542 at 1/50 (10.08 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing membranal and cytoplasmic staining in A549 cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Neogenin antibody [EPR25220-56] (ab302542), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Neogenin antibody [EPR25220-56] (ab302542)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SW480 (human colorectal adenocarcinoma epithelial cell) cells labelling Neogenin with ab302542 at 1/50 (10.08 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing membranal and cytoplasmic staining in SW480 cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

  • Western blot - Anti-Neogenin antibody [EPR25220-56] (ab302542), expandable thumbnail

    Western blot - Anti-Neogenin antibody [EPR25220-56] (ab302542)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-Neogenin antibody [EPR25220-56] (ab302542) at 1/1000 dilution

    Lane 1: His-tagged human Neogenin recombinant protein 10 ng

    Lane 2: His-tagged human DCC recombinant protein 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 60 kDa

    Exposure time: 3min

  • Western blot - Anti-Neogenin antibody [EPR25220-56] (ab302542), expandable thumbnail

    Western blot - Anti-Neogenin antibody [EPR25220-56] (ab302542)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression molecular weight observed is consistent with what has been described in the literature (PMID: 30858446, 33088218).

    All lanes: Western blot - Anti-Neogenin antibody [EPR25220-56] (ab302542) at 1/1000 dilution

    Lane 1: SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate 20 µg

    Lane 2: Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate 20 µg

    Lane 3: Untreated A549 (human lu carcinoma epithelial cell) whole cell lysate 20 µg

    Lane 4: A549 whole cell lysate treated with Protein Deglycosylation MIX II, 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 210 kDa, 160 kDa

    Exposure time: 3min

  • Western blot - Anti-Neogenin antibody [EPR25220-56] (ab302542), expandable thumbnail

    Western blot - Anti-Neogenin antibody [EPR25220-56] (ab302542)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-Neogenin antibody [EPR25220-56] (ab302542) at 1/1000 dilution

    Lane 1: A549 (human lu carcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate 20 µg

    Lane 2: A549 transfected with siRNA specifically targeting Neogenin), whole cell lysate 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 210 kDa

    Exposure time: 26s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com