AB216692

Anti-Nephrin antibody

Name: Anti-Nephrin antibody (ab216692) | Abcam
Brand: Abcam Limited
SKU: AB216692
Price: 545 USD
Availability: InStock

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(9 Publications)

Rabbit Polyclonal Nephrin antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Rat, Dog, Mouse samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human NPHS1 aa 450-550 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

NPHN, NPHS1, Nephrin, Renal glomerulus-specific cell adhesion receptor

3 Images

Flow Cytometry - Anti-Nephrin antibody (AB216692)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Nephrin antibody (AB216692)

Flow cytometric analysis of rat RSC96 cells labeling Nephrin using ab216692 at 1/100 dilution for 30 minutes (green), followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue) and isotype control (orange).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nephrin antibody (AB216692)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nephrin antibody (AB216692)

Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse kidney tissue labeling Nephrin using ab216692 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Lab

Western blot - Anti-Nephrin antibody (AB216692)

All lanes:

Western blot - Anti-Nephrin antibody (ab216692) at 1/500 dilution

All lanes:

MDCK cell lysates

Predicted band size: 134 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Dog

Applications

Flow Cyt, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human NPHS1 aa 450-550 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O60500

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nephrin also known as NPHS1 is a transmembrane protein with a molecular weight of approximately 180 kDa. Researchers have predominantly observed nephrin in the podocytes of the kidney glomerulus. Nephrin plays a mechanical role as part of the slit diaphragm a structure critical for maintaining the filtration barrier of the kidney. Its structure includes multiple immunoglobulin-like domains which facilitate cell signaling and adhesion processes important for podocyte function.

Biological function summary

Nephrin is essential for the regulation of glomerular permeability and podocyte foot process architecture. It serves as a significant component of the slit diaphragm complex working alongside proteins like podocin and CD2AP. This protein complex helps regulate the integrity and selectivity of the glomerular filtration barrier ensuring that blood plasma contents are selectively filtered while preventing the loss of large proteins into urine.

Pathways

Nephrin is integral to the PI3K/Akt signaling pathway influencing cell survival and cytoskeletal organization in podocytes. Nephrin's interaction within this pathway involves related proteins such as GAPDH and PTPRO which impact cellular functions critical to maintaining kidney structure and function. Additionally nephrin participates in the synaptopodin pathway modulating actin dynamics and therefore playing a role in cellular contraction and movement.

Nephrin dysfunction is closely related to congenital nephrotic syndrome particularly Finnish-type nephrosis which results in significant proteinuria and kidney failure. Nephrin mutations or aberrant expression can also contribute to other glomerular diseases such as focal segmental glomerulosclerosis (FSGS). In these conditions nephrin's relationship with other proteins like neph1 and podocin becomes disrupted leading to loss of slit diaphragm integrity and impaired renal function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity).

See full target information NPHS1

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40940806

2025

Development and Characterization of a Novel Lineage of Renal Progenitor Cells for Potential Use in Feline Chronic Kidney Disease: A Preliminary Study.

Applications

Unspecified application

Species

Unspecified reactive species

Lara Carolina Mario,Juliana de Paula Nhanharelli,Jéssica Borghesi,Rafaela Rodrigues Ribeiro,Hianka Jasmyne Costa de Carvalho,Thamires Santos da Silva,Mariano Del Sol,Rodrigo da Silva Nunes Barreto,Sandra Maria Barbalho,Maria Angelica Miglino

International journal of molecular sciences 26: PubMed40650083

2025

Network Pharmacology, Molecular Dynamics Simulation, and Biological Validation Insights into the Potential of Ligustri Lucidi Fructus for Diabetic Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Manting Liu,Yuhao Gu,Yuchang Yang,Ke Zhang,Jingwen Yang,Wenqi Wang,Wenjing Li,Xinzhu Wang,Xiaoxv Dong,Xingbin Yin,Changhai Qu,Boran Ni,Jian Ni

Scientific reports 15:1218 PubMed39774961

2025

METTL10 attenuates adriamycin-induced podocyte injury by targeting cell dedifferentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Jiwen Bao,Ziyang Li,Huanzhen Yao,Bei Wu,Leyi Gu,Yangbin Pan,Ling Wang

Cell death & disease 13:663 PubMed35908070

2022

Sestrin2 remedies podocyte injury via orchestrating TSP-1/TGF-β1/Smad3 axis in diabetic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Song,Chonglin Shi,Yawei Bian,Zhaohua Yang,Lin Mu,Haijiang Wu,Huijun Duan,Yonghong Shi

Bioengineered 13:12275-12288 PubMed35603354

2022

Triptolide inhibits oxidative stress and inflammation via the microRNA-155-5p/brain-derived neurotrophic factor to reduce podocyte injury in mice with diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Gao,Zheng Liang,Fei Zhao,Xiaojing Liu,Ning Ma

BMC nephrology 23:151 PubMed35436879

2022

Impact of klotho on the expression of SRGAP2a in podocytes in diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Donghua Jin,Miao Jia,Yuxian Xie,Lihua Lin,Hong Qiu,Guoyuan Lu

Chemico-biological interactions 308:89-100 PubMed31100273

2019

Protective effect of morin on doxorubicin-induced hepatorenal toxicity in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Müslüm Kuzu,Serkan Yıldırım,Fatih Mehmet Kandemir,Sefa Küçükler,Cüneyt Çağlayan,Erdinç Türk,Muhammet Bahaeddin Dörtbudak

American journal of physiology. Renal physiology 316:F1026-F1040 PubMed30810063

2019

Podocyte-specific expression of Cre recombinase promotes glomerular basement membrane thickening.

Applications

Unspecified application

Species

Unspecified reactive species

Rohan S Balkawade,Chao Chen,Michael R Crowley,David K Crossman,William L Clapp,Jill W Verlander,Caroline B Marshall

British journal of pharmacology 175:4167-4182 PubMed30079544

2018

Successful treatment of established heart failure in mice with recombinant HDL (Milano).

Applications

Unspecified application

Species

Unspecified reactive species

Joseph Pierre Aboumsallem,Mudit Mishra,Ruhul Amin,Ilayaraja Muthuramu,Herman Kempen,Bart De Geest

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nephrin antibody

Flow Cytometry - Anti-Nephrin antibody (AB216692)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nephrin antibody (AB216692)

Western blot - Anti-Nephrin antibody (AB216692)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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