AB183099

Anti-Nephrin antibody - C-terminal

Name: Anti-Nephrin antibody - C-terminal (ab183099) | Abcam
Brand: Abcam Limited
SKU: AB183099
Price: 535 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(7 Publications)

Rabbit Polyclonal Nephrin antibody. C-terminal. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human NPHS1 aa 1200 to C-terminus.

View Alternative Names

NPHN, NPHS1, Nephrin, Renal glomerulus-specific cell adhesion receptor

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nephrin antibody - C-terminal (AB183099)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nephrin antibody - C-terminal (AB183099)

Immunohistochemical analysis of paraffin embedded Human liver cancer tissue labeling Nephrin with ab183099 at 1/50 dilution. Image on the right is treated with the synthetic peptide.

Supplier Data

Western blot - Anti-Nephrin antibody - C-terminal (AB183099)

8% SDS-PAGE

All lanes:

Western blot - Anti-Nephrin antibody - C-terminal (ab183099) at 1/800 dilution

Lane 1:

Mouse spleen tissue lysate at 40 µg

Lane 2:

Mouse kidney tissue lysate at 40 µg

Secondary

All lanes:

Goat anti Rabbit IgG - H&L (HRP) at 1/5000 dilution

Predicted band size: 134 kDa

false

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human NPHS1 aa 1200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O60500

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nephrin also known as NPHS1 is a transmembrane protein with a molecular weight of approximately 180 kDa. Researchers have predominantly observed nephrin in the podocytes of the kidney glomerulus. Nephrin plays a mechanical role as part of the slit diaphragm a structure critical for maintaining the filtration barrier of the kidney. Its structure includes multiple immunoglobulin-like domains which facilitate cell signaling and adhesion processes important for podocyte function.

Biological function summary

Nephrin is essential for the regulation of glomerular permeability and podocyte foot process architecture. It serves as a significant component of the slit diaphragm complex working alongside proteins like podocin and CD2AP. This protein complex helps regulate the integrity and selectivity of the glomerular filtration barrier ensuring that blood plasma contents are selectively filtered while preventing the loss of large proteins into urine.

Pathways

Nephrin is integral to the PI3K/Akt signaling pathway influencing cell survival and cytoskeletal organization in podocytes. Nephrin's interaction within this pathway involves related proteins such as GAPDH and PTPRO which impact cellular functions critical to maintaining kidney structure and function. Additionally nephrin participates in the synaptopodin pathway modulating actin dynamics and therefore playing a role in cellular contraction and movement.

Nephrin dysfunction is closely related to congenital nephrotic syndrome particularly Finnish-type nephrosis which results in significant proteinuria and kidney failure. Nephrin mutations or aberrant expression can also contribute to other glomerular diseases such as focal segmental glomerulosclerosis (FSGS). In these conditions nephrin's relationship with other proteins like neph1 and podocin becomes disrupted leading to loss of slit diaphragm integrity and impaired renal function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity).

See full target information NPHS1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Methods in molecular biology (Clifton, N.J.) 2924:45-57 PubMed40307634

2025

One-Step Differentiation of Human-Induced Pluripotent Stem Cells into Podocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Elisabeth Naderlinger,Cormac Murphy,Elisabeth Feifel,Paul Jennings,Gerhard Gstraunthaler,Anja Wilmes

ALTEX 40:141-159 PubMed35791294

2022

Comparison of human recombinant protein coatings and fibroblast-ECM to Matrigel for induced pluripotent stem cell culture and renal podocyte differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Cormac Murphy,Elisabeth Naderlinger,Amber Mater,Roelof J C Kluin,Anja Wilmes

IET nanobiotechnology 15:473-483 PubMed34694755

2021

Alleviation of diabetic nephropathy by zinc oxide nanoparticles in streptozotocin-induced type 1 diabetes in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ghada Alomari,Bahaa Al-Trad,Salehhuddin Hamdan,Alaa A A Aljabali,Mazhar Salim Al Zoubi,Khalid Al-Batanyeh,Janti Qar,Gregory J Eaton,Almuthanna K Alkaraki,Walhan Alshaer,Saja Haifawi,Khairunadwa Jemon,Dinesh Kumar Chellappan,Kamal Dua,Murtaza M Tambuwala

Drug delivery and translational research 10:216-226 PubMed31637677

2019

Gold nanoparticles attenuate albuminuria by inhibiting podocyte injury in a rat model of diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Ghada Alomari,Bahaa Al-Trad,Salehhuddin Hamdan,Alaa Aljabali,Mazhar Al-Zoubi,Nesreen Bataineh,Janti Qar,Murtaza M Tambuwala

PloS one 13:e0203869 PubMed30222766

2018

Differentiation of human iPSCs into functional podocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline Rauch,Elisabeth Feifel,Georg Kern,Cormac Murphy,Florian Meier,Walther Parson,Mario Beilmann,Paul Jennings,Gerhard Gstraunthaler,Anja Wilmes

Evidence-based complementary and alternative medic 2016:5031890 PubMed27403197

2016

JiaWeiDangGui Decoction Ameliorates Proteinuria and Kidney Injury in Adriamycin-Induced Rat by Blockade of TGF-β/Smad Signaling.

Applications

IHC-P

Species

Rat

Ming-Gang Wei,Wei-Ming He,Xun Lu,Li Ni,Yan-Yu Yang,Lin Chen,Pei-Hua Xiong,Wei Sun

Diabetology & metabolic syndrome 7:97 PubMed26583047

2015

Progesterone ameliorates diabetic nephropathy in streptozotocin-induced diabetic Rats.

Applications

IHC-P

Species

Unspecified reactive species

Bahaa Al-Trad,Ibraheem M Ashankyty,Mohd Alaraj

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nephrin antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nephrin antibody - C-terminal (AB183099)

Western blot - Anti-Nephrin antibody - C-terminal (AB183099)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise