Anti-Nephrin antibody [Y17-R]

• WB

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Western blot - Anti-Nephrin antibody [Y17-R] (AB136894)

All lanes:

Western blot - Anti-Nephrin antibody [Y17-R] (ab136894) at 1/5000 dilution

Lane 1:

Recombinant Mouse Nephrin (extracellular domain, Gln 37 – Thr 1049) - 50ng

Lane 2:

Recombinant Mouse Nephrin (extracellular domain, Gln 37 – Thr 1049) - 100ng

Lane 3:

Recombinant Mouse Nephrin (extracellular domain, Gln 37 – Thr 1049) - 200ng

Predicted band size: 134 kDa

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• Flow Cyt

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Flow Cytometry - Anti-Nephrin antibody [Y17-R] (AB136894)

Overlay histogram showing HEK293 cells stained with ab136894 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab136894, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 80% methanol (5 min) fixed HEK293 cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.