AB80299

Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1]

RabMAb
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(11 Publications)

Rabbit Monoclonal Nephrin phospho Y1176 + Y1193 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 11 publications.

View Alternative Names

NPHN, NPHS1, Nephrin, Renal glomerulus-specific cell adhesion receptor

4 Images

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Western blot - Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (AB80299)

All lanes:

Western blot - Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299) at 1/50000 dilution

Lane 1:

HEK293T (human embryonic kidney epithelial cell) transfected with wild type CD 16/7 Nephrin expression vector, whole cell lysate

Lane 2:

HEK293T (human embryonic kidney epithelial cell) transfected with wild type CD 16/7 Nephrin and active tyrosine kinase (v-Src) expression vector, whole cell lysate

Lane 3:

HEK293T (human embryonic kidney epithelial cell) transfected with Y1176F mutant CD 16/7 Nephrin and active tyrosine kinase (v-Src) expression vector, whole cell lysate

Lane 4:

HEK293T (human embryonic kidney epithelial cell) transfected with Y1193F mutant CD 16/7 Nephrin and active tyrosine kinase (v-Src) expression vector, whole cell lysate

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 134 kDa

Observed band size: 138 kDa,180 kDa

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Western blot - Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (AB80299)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299) at 1/1000 dilution

Lane 1:

HEK293T (human embryonic kidney epithelial cell) transfected with myc-tagged human Nephrin expression vector, whole cell lysate at 15 µg

Lane 2:

HEK293T (human embryonic kidney epithelial cell) transfected with myc-tagged human Nephrin and myc-tagged Src expression vector, whole cell lysate at 15 µg

Lane 3:

HEK293T (human embryonic kidney epithelial cell) transfected with myc-tagged human Nephrin and myc-tagged Src expression vector, whole cell lysate. Then the membrane was incubated with phosphatase. at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 134 kDa

Observed band size: 170 kDa

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Dot Blot - Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (AB80299)

Dot blot analysis of Nephrin (pY1176 + pY1193) phospho peptide (Lane 1), Nephrin (pY1176) phospho peptide (Lane 2), Nephrin (pY1193) phospho peptide (Lane 3) and Nephrin non-phospho peptide (Lane 4) labelling Nephrin (pY1176 + pY1193) with ab80299 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer : 5% NFDM/TBST. Dilution buffer : 5% NFDM /TBST.

CiteAb

Western blot - Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (AB80299)

Nephrin (phospho Y1176 + Y1193) western blot using anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] ab80299. Publication image and figure legend from Lin, J. S., Jeon, J. S., et al., 2017, PLoS One, PubMed 28880939.

ab80299 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab80299 please see the product overview.

Nephrin tyrosine phosphorylation increased ARF6 activity and nephrin-ARF6 association.A. Immunoblot (IB) of total nephrin and tyrosine-phosphorylated nephrin (p-Nephrin) using the CD16/7-nephrin activation model in CD16/7-nephrin expressing human WT podocytes. B. Densitometry analysis of p-nephrin to total nephrin normalized to 0 minutes. Data are shown as mean ± SEM. C. Following nephrin induction in WT podocytes ARF6 activity was assessed by an ARF6 activation assay. GTPγs- and GDP-treated samples were used as positive and negative controls, respectively. D. Densitometry analysis of ARF6-GTP to total ARF6 normalized to 0 minutes. E. CD16/7-nephrin-ARF6 association was assessed by co-immunoprecipitation (IP) where nephrin was immunoprecipitated from podocyte lysates, and nephrin and ARF6 were detected by IB. IP using rabbit IgG was employed as control. F. Densitometry analysis of ARF6 normalized to nephrin. Assays were repeated in triplicate.

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Carrier free

Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPTPG1

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody detects Nephrin when either tyrosines 1176 or 1193 are phosphorylated, but does not detect non-phosphorylated Nephrin.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" }, "Mouse": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nephrin also known as NPHS1 is a transmembrane protein with a molecular weight of approximately 180 kDa. Researchers have predominantly observed nephrin in the podocytes of the kidney glomerulus. Nephrin plays a mechanical role as part of the slit diaphragm a structure critical for maintaining the filtration barrier of the kidney. Its structure includes multiple immunoglobulin-like domains which facilitate cell signaling and adhesion processes important for podocyte function.

Biological function summary

Nephrin is essential for the regulation of glomerular permeability and podocyte foot process architecture. It serves as a significant component of the slit diaphragm complex working alongside proteins like podocin and CD2AP. This protein complex helps regulate the integrity and selectivity of the glomerular filtration barrier ensuring that blood plasma contents are selectively filtered while preventing the loss of large proteins into urine.

Pathways

Nephrin is integral to the PI3K/Akt signaling pathway influencing cell survival and cytoskeletal organization in podocytes. Nephrin's interaction within this pathway involves related proteins such as GAPDH and PTPRO which impact cellular functions critical to maintaining kidney structure and function. Additionally nephrin participates in the synaptopodin pathway modulating actin dynamics and therefore playing a role in cellular contraction and movement.

Nephrin dysfunction is closely related to congenital nephrotic syndrome particularly Finnish-type nephrosis which results in significant proteinuria and kidney failure. Nephrin mutations or aberrant expression can also contribute to other glomerular diseases such as focal segmental glomerulosclerosis (FSGS). In these conditions nephrin's relationship with other proteins like neph1 and podocin becomes disrupted leading to loss of slit diaphragm integrity and impaired renal function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity).

See full target information NPHS1 pY1176 + Y1193

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112673 PubMed40510123

2025

Reduced nephrin tyrosine phosphorylation impairs podocyte force transmission and accelerates detachment in disease.

Applications

Unspecified application

Species

Unspecified reactive species

Casey R Williamson,Claire E Martin,J Dinesh Kumar,Peihua Lu,Laura A New,Alice Y Wang,Nils M Kronenberg,Malte C Gather,Paul A Reynolds,Nina Jones

Cardiovascular diabetology 22:293 PubMed37891556

2023

Klotho inhibits renal ox-LDL deposition via IGF-1R/RAC1/OLR1 signaling to ameliorate podocyte injury in diabetic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Jiang,Chun Gan,Xindi Zhou,Qing Yang,Dan Chen,Han Xiao,Lujun Dai,Yaxi Chen,Mo Wang,Haiping Yang,Qiu Li

iScience 25:104694 PubMed35847557

2022

Pulmonary surfactants and the respiratory-renal connection in steroid-sensitive nephrotic syndrome of childhood.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriel Cara-Fuentes,Ana Andres-Hernando,Colin Bauer,Mindy Banks,Gabriela E Garcia,Christina Cicerchi,Masanari Kuwabara,Michiko Shimada,Richard J Johnson,Miguel A Lanaspa

Development (Cambridge, England) 149: PubMed35417019

2022

Human pluripotent stem cell-derived kidney organoids for personalized congenital and idiopathic nephrotic syndrome modeling.

Applications

Unspecified application

Species

Unspecified reactive species

Jitske Jansen,Bartholomeus T van den Berge,Martijn van den Broek,Rutger J Maas,Deniz Daviran,Brigith Willemsen,Rona Roverts,Marit van der Kruit,Christoph Kuppe,Katharina C Reimer,Gianluca Di Giovanni,Fieke Mooren,Quincy Nlandu,Helmer Mudde,Roy Wetzels,Dirk den Braanker,Naomi Parr,James S Nagai,Vedran Drenic,Ivan G Costa,Eric Steenbergen,Tom Nijenhuis,Henry Dijkman,Nicole Endlich,Nicole C A J van de Kar,Rebekka K Schneider,Jack F M Wetzels,Anat Akiva,Johan van der Vlag,Rafael Kramann,Michiel F Schreuder,Bart Smeets

The Journal of biological chemistry 297:101079 PubMed34391780

2021

Phosphorylation of slit diaphragm proteins NEPHRIN and NEPH1 upon binding of HGF promotes podocyte repair.

Applications

Unspecified application

Species

Unspecified reactive species

Ashish K Solanki,Ehtesham Arif,Pankaj Srivastava,Christopher M Furcht,Bushra Rahman,Pei Wen,Avinash Singh,Lawrence B Holzman,Wayne R Fitzgibbon,Milos N Budisavljevic,Glenn P Lobo,Sang-Ho Kwon,Zhe Han,Matthew J Lazzara,Joshua H Lipschutz,Deepak Nihalani

Journal of cell science 133: PubMed31974115

2020

Multivalent nephrin-Nck interactions define a threshold for clustering and tyrosine-dependent nephrin endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Claire E Martin,Laura A New,Noah J Phippen,Ava Keyvani Chahi,Alexander E Mitro,Tomoko Takano,Tony Pawson,Ivan M Blasutig,Nina Jones

Proceedings of the National Academy of Sciences of 116:3712-3721 PubMed30733285

2019

Recruitment of APOL1 kidney disease risk variants to lipid droplets attenuates cell toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Justin Chun,Jia-Yue Zhang,Maris S Wilkins,Balajikarthick Subramanian,Cristian Riella,Jose M Magraner,Seth L Alper,David J Friedman,Martin R Pollak

Journal of the American Society of Nephrology : JA 29:2641-2657 PubMed30341149

2018

SHROOM3-FYN Interaction Regulates Nephrin Phosphorylation and Affects Albuminuria in Allografts.

Applications

Unspecified application

Species

Unspecified reactive species

Chengguo Wei,Khadija Banu,Felipe Garzon,John M Basgen,Nimrod Philippe,Zhengzi Yi,Ruijie Liu,Jui Choudhuri,Miguel Fribourg,Tong Liu,Arun Cumpelik,Jenny Wong,Mubeen Khan,Bhaskar Das,Karen Keung,Fadi Salem,Kirk N Campbell,Lewis Kaufman,Paolo Cravedi,Weijia Zhang,Philip J O'Connell,John Cijiang He,Barbara Murphy,Madhav C Menon

PloS one 12:e0184575 PubMed28880939

2017

ARF6 mediates nephrin tyrosine phosphorylation-induced podocyte cellular dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Jamie S Lin,Jin Seok Jeon,Qingfeng Fan,Hetty N Wong,Matthew B Palmer,Lawrence B Holzman

Molecular and cellular endocrinology 400:112-28 PubMed25448064

2014

Nephrin, a transmembrane protein, is involved in pancreatic beta-cell survival signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Katerina Kapodistria,Effie-Photini Tsilibary,Panagiotis Politis,Petros Moustardas,Aristidis Charonis,Paraskevi Kitsiou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1]

Western blot - Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (AB80299)

Western blot - Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (AB80299)

Dot Blot - Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (AB80299)

Western blot - Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (AB80299)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise