AB80298

Anti-Nephrin (phospho Y1217) antibody [EPTPG3]

RabMAb
Recombinant
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(10 Publications)

Rabbit Recombinant Monoclonal Nephrin phospho Y1217 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Transfected cell lysate - Human samples. Cited in 10 publications.

View Alternative Names

NPHN, NPHS1, Nephrin, Renal glomerulus-specific cell adhesion receptor

4 Images

Supplier Data

Western blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (AB80298)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (ab80298) at 1/10000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) transfected with a myc-tagged empty vector whole cell lysate at 15 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) transfected with with myc-tagged human Nephrin and myc-tagged Src expression vectors, whole cell lysate at 15 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) transfected with myc-tagged human Nephrin and myc-tagged Src expression vectors, whole cell lysate. Then the membrane was incubated with Alkaline phosphatase at 15 µg

Predicted band size: 134 kDa

Observed band size: 170 kDa

false

Supplier Data

Western blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (AB80298)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Predicted band size: 134 kDa

Observed band size: 170 kDa

false

Supplier Data

Dot Blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (AB80298)

Dot blot analysis of Nephrin (pY1217) peptide and Nephrin non-phospho peptide labelling PKC alpha Nephrin (pY1217) with ab80298 at a 1/1000 dilution. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a 1/20,000 dilution. Blocking and dilution buffer : 5% NFDM/TBST.

Lane 1 : Nephrin (pY1217) phospho peptide.

Lane 2 : Nephrin non-phospho peptide.

Unknown

Dot Blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (AB80298)

Dot blot analysis of Nephrin (pY1217) phospho peptide (Lane 1) and Nephrin non-phospho peptide (Lane 2) labelling Nephrin (pY1217) with ab80298 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer : 5% NFDM/TBST. Dilution buffer : 5% NFDM /TBST.

Carrier free

Anti-Nephrin (phospho Y1217) antibody [EPTPG3] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPTPG3

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Human": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

This product has switched from a hybridoma to recombinant production method on 20th November 2023.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nephrin also known as NPHS1 is a transmembrane protein with a molecular weight of approximately 180 kDa. Researchers have predominantly observed nephrin in the podocytes of the kidney glomerulus. Nephrin plays a mechanical role as part of the slit diaphragm a structure critical for maintaining the filtration barrier of the kidney. Its structure includes multiple immunoglobulin-like domains which facilitate cell signaling and adhesion processes important for podocyte function.

Biological function summary

Nephrin is essential for the regulation of glomerular permeability and podocyte foot process architecture. It serves as a significant component of the slit diaphragm complex working alongside proteins like podocin and CD2AP. This protein complex helps regulate the integrity and selectivity of the glomerular filtration barrier ensuring that blood plasma contents are selectively filtered while preventing the loss of large proteins into urine.

Pathways

Nephrin is integral to the PI3K/Akt signaling pathway influencing cell survival and cytoskeletal organization in podocytes. Nephrin's interaction within this pathway involves related proteins such as GAPDH and PTPRO which impact cellular functions critical to maintaining kidney structure and function. Additionally nephrin participates in the synaptopodin pathway modulating actin dynamics and therefore playing a role in cellular contraction and movement.

Nephrin dysfunction is closely related to congenital nephrotic syndrome particularly Finnish-type nephrosis which results in significant proteinuria and kidney failure. Nephrin mutations or aberrant expression can also contribute to other glomerular diseases such as focal segmental glomerulosclerosis (FSGS). In these conditions nephrin's relationship with other proteins like neph1 and podocin becomes disrupted leading to loss of slit diaphragm integrity and impaired renal function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity).

See full target information NPHS1 phospho Y1217

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112673 PubMed40510123

2025

Reduced nephrin tyrosine phosphorylation impairs podocyte force transmission and accelerates detachment in disease.

Applications

Unspecified application

Species

Unspecified reactive species

Casey R Williamson,Claire E Martin,J Dinesh Kumar,Peihua Lu,Laura A New,Alice Y Wang,Nils M Kronenberg,Malte C Gather,Paul A Reynolds,Nina Jones

Frontiers in cell and developmental biology 10:790365 PubMed35372328

2022

Rap1 Activity Is Essential for Focal Adhesion and Slit Diaphragm Integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Mee-Ling Maywald,Cara Picciotto,Carolin Lepa,Luisa Bertgen,Farwah Sanam Yousaf,Andrea Ricker,Jürgen Klingauf,Michael P Krahn,Hermann Pavenstädt,Britta George

The Journal of biological chemistry 297:101079 PubMed34391780

2021

Phosphorylation of slit diaphragm proteins NEPHRIN and NEPH1 upon binding of HGF promotes podocyte repair.

Applications

Unspecified application

Species

Unspecified reactive species

Ashish K Solanki,Ehtesham Arif,Pankaj Srivastava,Christopher M Furcht,Bushra Rahman,Pei Wen,Avinash Singh,Lawrence B Holzman,Wayne R Fitzgibbon,Milos N Budisavljevic,Glenn P Lobo,Sang-Ho Kwon,Zhe Han,Matthew J Lazzara,Joshua H Lipschutz,Deepak Nihalani

Cell death & disease 11:355 PubMed32393782

2020

Role of pyruvate kinase M2-mediated metabolic reprogramming during podocyte differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Yuan,Jiao Miao,Qianqian Yang,Li Fang,Yi Fang,Hao Ding,Yang Zhou,Lei Jiang,Chunsun Dai,Ke Zen,Qi Sun,Junwei Yang

Cell death & disease 11:319 PubMed32371936

2020

Nestin protects podocyte from injury in lupus nephritis by mitophagy and oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yuexin Tian,Huifang Guo,Xinyan Miao,Jie Xu,Ran Yang,Lu Zhao,Jinxi Liu,Lin Yang,Fan Gao,Wei Zhang,Qingjuan Liu,Shaoguang Sun,Yu Tian,Hongbo Li,Jie Huang,Cunyang Gu,Shuxia Liu,Xiaojuan Feng

Journal of the American Society of Nephrology : JA 27:2422-35 PubMed26802179

2016

Nephrin Tyrosine Phosphorylation Is Required to Stabilize and Restore Podocyte Foot Process Architecture.

Applications

Unspecified application

Species

Unspecified reactive species

Laura A New,Claire E Martin,Rizaldy P Scott,Mathew J Platt,Ava Keyvani Chahi,Colin D Stringer,Peihua Lu,Bozena Samborska,Vera Eremina,Tomoko Takano,Jeremy A Simpson,Susan E Quaggin,Nina Jones

American journal of physiology. Renal physiology 307:F634-47 PubMed25056348

2014

Complement-mediated glomerular injury is reduced by inhibition of protein-tyrosine phosphatase 1B.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa Nezvitsky,Michel L Tremblay,Tomoko Takano,Joan Papillon,Andrey V Cybulsky

The American journal of pathology 184:2211-24 PubMed24951831

2014

Protein tyrosine phosphatase 1B inhibition protects against podocyte injury and proteinuria.

Applications

Unspecified application

Species

Unspecified reactive species

Takanori Kumagai,Cindy Baldwin,Lamine Aoudjit,Lisa Nezvitsky,Richard Robins,Ruihua Jiang,Tomoko Takano

The Journal of biological chemistry 287:28932-42 PubMed22718751

2012

Dynamin-mediated Nephrin phosphorylation regulates glucose-stimulated insulin release in pancreatic beta cells.

Applications

Species

Human

Jongmin Jeon,Ingo Leibiger,Tilo Moede,Britta Walter,Christian Faul,Dony Maiguel,Rodrigo Villarreal,Johanna Guzman,Per-Olof Berggren,Peter Mundel,Camillo Ricordi,Sandra Merscher-Gomez,Alessia Fornoni

American journal of physiology. Cell physiology 301:C1351-9 PubMed21881001

2011

TM4SF10 and ADAP interaction in podocytes: role in Fyn activity and nephrin phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Timur A Azhibekov,Zhenzhen Wu,Aparna Padiyar,Leslie A Bruggeman,Jeffrey S Simske

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nephrin (phospho Y1217) antibody [EPTPG3]

Western blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (AB80298)

Western blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (AB80298)

Dot Blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (AB80298)

Dot Blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (AB80298)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise