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AB213689

Anti-Neprilysin-2 antibody

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(1 Publication)

Rabbit Polyclonal Neprilysin-2 antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MMEL1 aa 450-550 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

MELL1, MMEL2, NEP2, MMEL1, Membrane metallo-endopeptidase-like 1, Membrane metallo-endopeptidase-like 2, NEP2(m), Neprilysin II, Neprilysin-2, NEPII, NL2

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neprilysin-2 antibody (AB213689)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neprilysin-2 antibody (AB213689)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat myocardium tissue labeling Neprilysin-2 with ab213689 at 1/200 dilution, followed by conjugation to a secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human MMEL1 aa 450-550 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q495T6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neprilysin-2 also known as NEP2 or MMEL1 is a metalloendopeptidase enzyme similar to neprilysin. It has an approximate mass of 85 kDa. The enzyme is expressed in various tissues with a notable presence in the brain. NEP2 plays an important role in peptide degradation acting on a range of bioactive peptides by cleaving peptide bonds.
Biological function summary

NEP2 functions to break down peptides that are involved in signal transduction and cell communication. It participates in the regulation of neuropeptide signaling. NEP2 does not work alone but forms complexes with other proteins to perform its tasks efficiently. This activity allows it to maintain balance in peptide hormone levels and influence multiple physiological processes.

Pathways

Neprilysin-2 serves as a critical component in the metabolism of neuropeptides especially within the renin-angiotensin system and opioid peptide pathways. In these pathways NEP2 processes peptides like enkephalins and angiotensins directly interacting with other enzymes such as neprilysin and insulin-degrading enzyme. These interactions help modulate cardiovascular and nociceptive systems.

Neprilysin-2 associates with neurodegenerative diseases and cardiovascular disorders. Alteration in NEP2 activity is linked to Alzheimer's disease through its involvement in the degradation of amyloid-beta peptides. Its relation to neprilysin is significant because these enzymes together regulate peptides implicated in these conditions. Abnormal NEP2 function can lead to disrupted peptide balance contributing to disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Metalloprotease involved in sperm function, possibly by modulating the processes of fertilization and early embryonic development. Degrades a broad variety of small peptides with a preference for peptides shorter than 3 kDa containing neutral bulky aliphatic or aromatic amino acid residues. Shares the same substrate specificity with MME and cleaves peptides at the same amide bond (By similarity).
See full target information MMEL1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 14: PubMed39334940

2024

Connexin Expression Is Altered in the Eye Development of Mice: A Preliminary Study.

Applications

Unspecified application

Species

Unspecified reactive species

Ljubica Skelin,Anita Racetin,Nela Kelam,Marin Ogorevc,Ljubo Znaor,Mirna Saraga-Babić,Natalija Filipović,Yu Katsuyama,Zenon Pogorelić,Katarina Vukojević
View all publications
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Product promise

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