Anti-Nesprin 2 antibody

• WB

Lab

Western blot - Anti-Nesprin 2 antibody (AB217057)

Western blot : Anti-SYNE2 antibody (ab217057) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab217057 was shown to bind specifically to SYNE2. A band was observed at 796 kDa in wild-type A549 cell lysates with no signal observed at this size in SYNE2 knockout cell line. To generate this image, wild-type and SYNE2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Nesprin 2 antibody (ab217057) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

SYNE2 knockout A549 cell lysate at 20 µg

Lane 3:

U-2 OS cell lysate at 20 µg

Lane 4:

THP-1 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 796 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-Nesprin 2 antibody (AB217057)

Immunoprecipitation analysis of HeLa cell lysate (in NETN buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling Nesprin 2 using ab217057 at 6 μg per reaction (lane 1). A control IgG was used in lane 2. For blotting immunoprecipitated Nesprin 2, ab217057 was used at 1 μg/mL. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-Nesprin 2 antibody (ab217057)

Predicted band size: 796 kDa

false

• WB

Supplier Data

Western blot - Anti-Nesprin 2 antibody (AB217057)

cells prepared using NETN lysis buffer

All lanes:

Western blot - Anti-Nesprin 2 antibody (ab217057) at 0.4 µg

Lane 1:

HeLa cell lysate at 50 µg

Lane 2:

293T cell lysate at 50 µg

Lane 3:

Jurkat cell lysate at 50 µg

Predicted band size: 796 kDa

true

Exposure time: 30s