AB233034

Anti-Nesprin 2 antibody

Name: Anti-Nesprin 2 antibody (ab233034) | Abcam
Brand: Abcam Limited
SKU: AB233034
Price: 510 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal Nesprin 2 antibody. Suitable for WB, IHC-P and reacts with Human, Pig, Recombinant fragment - Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SYNE2 aa 1-300.

View Alternative Names

KIAA1011, NUA, SYNE2, Nesprin-2, KASH domain-containing protein 2, Nuclear envelope spectrin repeat protein 2, Nucleus and actin connecting element protein, Synaptic nuclear envelope protein 2, KASH2, Protein NUANCE, Syne-2

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin 2 antibody (AB233034)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin 2 antibody (AB233034)

Formalin-fixed, paraffin-embedded human cerebrum tissue stained for Nesprin 2 using ab233034 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin 2 antibody (AB233034)

Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for Nesprin 2 using ab233034 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin 2 antibody (AB233034)

Formalin-fixed, paraffin-embedded human kidney tissue stained for Nesprin 2 using ab233034 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin 2 antibody (AB233034)

Formalin-fixed, paraffin-embedded human stomach tissue stained for Nesprin 2 using ab233034 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin 2 antibody (AB233034)

Formalin-fixed, paraffin-embedded human liver tissue stained for Nesprin 2 using ab233034 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin 2 antibody (AB233034)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Nesprin 2 using ab233034 at 10 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Nesprin 2 antibody (AB233034)

All lanes:

Western blot - Anti-Nesprin 2 antibody (ab233034) at 1 µg/mL

All lanes:

Recombinant human Nesprin 2 protein

Predicted band size: 796 kDa

false

Supplier Data

Western blot - Anti-Nesprin 2 antibody (AB233034)

All lanes:

Western blot - Anti-Nesprin 2 antibody (ab233034) at 1 µg/mL

Lane 1:

Human lung lysate

Lane 2:

A431 (human epidermoid carcinoma cell line) cell lysate

Lane 3:

Pig kidney lysate

Lane 4:

Pig stomach lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution

Predicted band size: 796 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SYNE2 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q8WXH0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nesprin-2 also known as Nesprin-2 or ABP-Rince2002 is a mechanical protein that plays an important role in the structure of cells. This protein is part of the spectrin repeat-containing nuclear envelope (KASH) proteins with a large mass. It is mainly expressed in tissues such as skeletal muscle the heart and brain. Nesprin-2 is found in the outer nuclear membrane where it functions as a mediator linking the nuclear envelope to the cytoskeleton facilitating mechanical coupling between the nucleus and the cytoplasm.

Biological function summary

Nesprin-2 is involved in various cellular processes that contribute to nuclear positioning and cell movement. It interacts with other nuclear envelope proteins like SUN proteins forming the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex which ensures mechanical communication within cells. This complex supports the anchoring and movement of the nucleus during cytoskeletal changes. Nesprin-2's anchoring abilities make it essential for maintaining cellular integrity and adaptability during mechanical stress.

Pathways

Nesprin-2 participates in mechanotransduction pathways that convert mechanical stimuli into biochemical signals. It plays a critical role in the actin cytoskeleton organization and nuclear positioning pathways. Through these pathways Nesprin-2 interacts with proteins such as F-actin which are important for maintaining cell shape and mobility. The interactions between Nesprin-2 and cytoskeletal elements help facilitate cell signaling in response to mechanical forces.

Nesprin-2 is linked to muscular dystrophies and cardiomyopathies. In muscular dystrophies mutations in Nesprin-2 or its interacting partners like Emerin impair nuclear-cytoskeletal coupling leading to muscle weakness. In cardiomyopathies Nesprin-2 dysfunction affects cardiac muscle integrity and function where it may interact with proteins like Lamin A/C. Understanding these connections offers potential avenues for researching therapeutic interventions for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning (PubMed : 34818527). Specifically, SYNE2 and SUN2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. May be involved in nucleus-centrosome attachment. During interkinetic nuclear migration (INM) at G2 phase and nuclear migration in neural progenitors its LINC complex association with SUN1/2 and probable association with cytoplasmic dynein-dynactin motor complexes functions to pull the nucleus toward the centrosome; SYNE1 and SYNE2 may act redundantly. During INM at G1 phase mediates respective LINC complex association with kinesin to push the nucleus away from the centrosome. Involved in nuclear migration in retinal photoreceptor progenitors. Required for centrosome migration to the apical cell surface during early ciliogenesis. Facilitates the relaxation of mechanical stress imposed by compressive actin fibers at the rupture site through its nteraction with SYN2 (PubMed : 34818527).

See full target information SYNE2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:6845 PubMed34824246

2021

Imprinted lncRNA Dio3os preprograms intergenerational brown fat development and obesity resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Ting Chen,Qi-Yuan Yang,Yun Hu,Xiang-Dong Liu,Jeanene M de Avila,Mei-Jun Zhu,Peter W Nathanielsz,Min Du

Advanced biology 5:e2000048 PubMed33724728

2021

Micropatterned Surfaces Expose the Coupling between Actin Cytoskeleton-Lamin/Nesprin and Nuclear Deformability of Breast Cancer Cells with Different Malignancies.

Applications

Unspecified application

Species

Unspecified reactive species

Ezgi Antmen,Utkan Demirci,Vasif Hasirci

Frontiers in pharmacology 11:617270 PubMed33584299

2021

USP7 Inhibition Alleviates HO-Induced Injury in Chondrocytes via Inhibiting NOX4/NLRP3 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Liu,Qingbai Liu,Bin Yan,Ziqiang Zhu,Yaozeng Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Nesprin 2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin 2 antibody (AB233034)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin 2 antibody (AB233034)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin 2 antibody (AB233034)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin 2 antibody (AB233034)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin 2 antibody (AB233034)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin 2 antibody (AB233034)

Western blot - Anti-Nesprin 2 antibody (AB233034)

Western blot - Anti-Nesprin 2 antibody (AB233034)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise