Mouse Monoclonal Nesprin3 antibody. Suitable for ICC/IF and reacts with Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Nesprin-3.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Nesprin3 antibody [Nsp3] (AB123031), expandable thumbnail](https://content.abcam.com/products/images/nesprin3-antibody-nsp3-ab123031--immunocytochemistry-immunofluorescence-img73671.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Nesprin3 antibody [Nsp3] (AB123031)")
Publications
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- Nature materials 22:1409-14202023The laminin-keratin link shields the nucleus from mechanical deformation and signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZanetta Kechagia,Pablo Sáez,Manuel Gómez-González,Brenda Canales,Srivatsava Viswanadha,Martín Zamarbide,Ion Andreu,Thijs Koorman,Amy E M Beedle,Alberto Elosegui-Artola,Patrick W B Derksen,Xavier Trepat,Marino Arroyo,Pere Roca-CusachsPubMed 37709930
- Nature communications 9:39952018Curvotaxis directs cell migration through cell-scale curvature landscapes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaurent Pieuchot et. al.PubMed 30266986
- Scientific reports 6:380632016The mammalian LINC complex regulates genome transcriptional responses to substrate rigidity.
- The EMBO journal 34:1875-882015Loss of MT1-MMP causes cell senescence and nuclear defects which can be reversed by retinoic acid.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna Gutiérrez-Fernández et. al.PubMed 25991604
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Mouse Nesprin-3. The exact immunogen used to generate this antibody is proprietary information. Database link Q4FZC9
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Not recommended |
| Mouse | Tested |
| Zebrafish | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish, Human | Dilution info - | Notes - |
Associated Products
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4 products for Alternative Product
Target data
Function
As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells (By similarity).
Alternative names
Nesprin-3, KASH domain-containing protein 3, Nuclear envelope spectrin repeat protein 3, KASH3, Syne3
Recommended products
Mouse Monoclonal Nesprin3 antibody. Suitable for ICC/IF and reacts with Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Nesprin-3.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Mouse Nesprin-3. The exact immunogen used to generate this antibody is proprietary information. Database link Q4FZC9
- Clone number
- Nsp3
- Purification technique
- Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nesprin-3 also known as SYNE3 is a mechanical adaptor protein of around 215 kDa. It belongs to the spectrin repeat family and is primarily expressed in the outer nuclear membrane. This protein anchors the nucleus to the cytoskeleton by interacting with cytoskeletal linkers like plectin. It plays a role in maintaining the structural integrity of the nucleus and cellular architecture due to these anchoring functions.
- Biological function summary
Nesprin-3 is involved in the linking of the nuclear envelope to the cytoskeleton and it forms part of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex. This complex is important for nuclear movement within cells and has a role in signal transduction from the cell surface to the nucleus. Through its ability to bind to other cytoskeletal elements Nesprin-3 contributes to force transmission across the cell impacting cell polarity and migration.
- Pathways
Nesprin-3 interacts with several cytoskeletal signaling pathways particularly the actin and intermediate filament pathways. It associates with proteins like plectin and vimentin playing significant parts in cellular mechanics and signaling. By engaging in these pathways Nesprin-3 helps control cellular responses to mechanical stress and influences various processes including cell migration and division.
- Associated diseases and disorders
Nesprin-3 has been linked to muscular dystrophies and certain cancers. In muscular dystrophies its interactions with plectin can become disrupted leading to compromised muscle integrity and function. In cancer changes in its expression or interactions may affect cell movement and invasion contributing to metastasis. Its role in these diseases highlights its importance in maintaining proper cell architecture and function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Immunocytochemistry/ Immunofluorescence - Anti-Nesprin3 antibody [Nsp3] (ab123031), expandable thumbnail](https://content.abcam.com/products/images/nesprin3-antibody-nsp3-ab123031--immunocytochemistry-immunofluorescence-img73671.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Nesprin3 antibody [Nsp3] (ab123031)")
Immunocytochemistry/ Immunofluorescence - Anti-Nesprin3 antibody [Nsp3] (ab123031)
ab123031 staining Nesprin3 in cultured mouse embryonic fibroblast by Immunofluorescence (green). The nuclear DNA is counterstained with DAPI (blue).
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