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AB123031

Anti-Nesprin3 antibody [Nsp3]

4

(1 Review)

|

(5 Publications)

Mouse Monoclonal Nesprin3 antibody. Suitable for ICC/IF and reacts with Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Nesprin-3.

View Alternative Names

Nesprin-3, KASH domain-containing protein 3, Nuclear envelope spectrin repeat protein 3, KASH3, Syne3

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Nesprin3 antibody [Nsp3] (AB123031)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Nesprin3 antibody [Nsp3] (AB123031)

ab123031 staining Nesprin3 in cultured mouse embryonic fibroblast by Immunofluorescence (green). The nuclear DNA is counterstained with DAPI (blue).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Nsp3

Isotype

IgG1

Carrier free

No

Reacts with

Mouse

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Mouse Nesprin-3. The exact immunogen used to generate this antibody is proprietary information.

Q4FZC9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Zebrafish": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nesprin-3 also known as SYNE3 is a mechanical adaptor protein of around 215 kDa. It belongs to the spectrin repeat family and is primarily expressed in the outer nuclear membrane. This protein anchors the nucleus to the cytoskeleton by interacting with cytoskeletal linkers like plectin. It plays a role in maintaining the structural integrity of the nucleus and cellular architecture due to these anchoring functions.
Biological function summary

Nesprin-3 is involved in the linking of the nuclear envelope to the cytoskeleton and it forms part of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex. This complex is important for nuclear movement within cells and has a role in signal transduction from the cell surface to the nucleus. Through its ability to bind to other cytoskeletal elements Nesprin-3 contributes to force transmission across the cell impacting cell polarity and migration.

Pathways

Nesprin-3 interacts with several cytoskeletal signaling pathways particularly the actin and intermediate filament pathways. It associates with proteins like plectin and vimentin playing significant parts in cellular mechanics and signaling. By engaging in these pathways Nesprin-3 helps control cellular responses to mechanical stress and influences various processes including cell migration and division.

Nesprin-3 has been linked to muscular dystrophies and certain cancers. In muscular dystrophies its interactions with plectin can become disrupted leading to compromised muscle integrity and function. In cancer changes in its expression or interactions may affect cell movement and invasion contributing to metastasis. Its role in these diseases highlights its importance in maintaining proper cell architecture and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells (By similarity).
See full target information Nesprin-3
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 223: PubMed38683248

2024

Nucleocytoplasmic transport rates are regulated by cellular processes that modulate GTP availability.

Applications

Unspecified application

Species

Unspecified reactive species

Kelsey L Scott,Charles T Halfmann,Allison D Hoefakker,Purboja Purkayastha,Ting Ching Wang,Tanmay P Lele,Kyle J Roux

Nature materials 22:1409-1420 PubMed37709930

2023

The laminin-keratin link shields the nucleus from mechanical deformation and signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Zanetta Kechagia,Pablo Sáez,Manuel Gómez-González,Brenda Canales,Srivatsava Viswanadha,Martín Zamarbide,Ion Andreu,Thijs Koorman,Amy E M Beedle,Alberto Elosegui-Artola,Patrick W B Derksen,Xavier Trepat,Marino Arroyo,Pere Roca-Cusachs

Nature communications 9:3995 PubMed30266986

2018

Curvotaxis directs cell migration through cell-scale curvature landscapes.

Applications

Unspecified application

Species

Unspecified reactive species

Laurent Pieuchot,Julie Marteau,Alain Guignandon,Thomas Dos Santos,Isabelle Brigaud,Pierre-François Chauvy,Thomas Cloatre,Arnaud Ponche,Tatiana Petithory,Pablo Rougerie,Maxime Vassaux,Jean-Louis Milan,Nayana Tusamda Wakhloo,Arnaud Spangenberg,Maxence Bigerelle,Karine Anselme

Scientific reports 6:38063 PubMed27905489

2016

The mammalian LINC complex regulates genome transcriptional responses to substrate rigidity.

Applications

ICC/IF

Species

Unspecified reactive species

Samer G Alam,Qiao Zhang,Nripesh Prasad,Yuan Li,Srikar Chamala,Ram Kuchibhotla,Birendra Kc,Varun Aggarwal,Shristi Shrestha,Angela L Jones,Shawn E Levy,Kyle J Roux,Jeffrey A Nickerson,Tanmay P Lele

The EMBO journal 34:1875-88 PubMed25991604

2015

Loss of MT1-MMP causes cell senescence and nuclear defects which can be reversed by retinoic acid.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Gutiérrez-Fernández,Clara Soria-Valles,Fernando G Osorio,Jesús Gutiérrez-Abril,Cecilia Garabaya,Alina Aguirre,Antonio Fueyo,María Soledad Fernández-García,Xose S Puente,Carlos López-Otín
View all publications
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