AB22035

Anti-Nestin antibody [10C2] - Neural Stem Cell Marker

Name: Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (ab22035) | Abcam
Brand: Abcam Limited
SKU: AB22035
Price: 400 USD
Availability: InStock
Rating: 4 (8 reviews)

Lab Essentials
What is this?

(8 Reviews)

(204 Publications)

Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (ab22035) is a mouse monoclonal antibody detecting Nestin in Western Blot. Suitable for Human.

- Over 150 publications
- Trusted since 2005

View Alternative Names

Nbla00170, NES, Nestin

4 Images

AbReview11450****

Immunocytochemistry - Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (AB22035)

ab22035 staining human brain tissue cells by ICC/IF. Cells were PFA fixed and permeabilized in Triton X-100 prior to blocking in 1% BSA for 30 minutes at 21°C. The primary antibody was diluted 1/100 and incubated with the samples for 1 hour at 21°C. A FITC conjugated goat anti-mouse IgG antibody, diluted 1/250, was used as the secondary.

This image is courtesy of an Abreview submitted by Dr Amal Shervington

Supplier Data

Immunocytochemistry - Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (AB22035)

Immunostaining of intracellular nestin filaments in cultured SH-SY-5Y neuronal cells using ab22035 (1 : 100 dilution).

Supplier Data

Immunocytochemistry - Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (AB22035)

ICC/IF of U251 cells staining intracellular Nestin filaments with ab22035

Unknown

Western blot - Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (AB22035)

Lane 1:

Western blot - Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (ab22035) at 1/1000 dilution

Lane 2:

Western blot - Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (ab22035)

Lane 1:

U251 cells at 10 µL

Lane 2:

U251 cells at 20 µL

Predicted band size: 177 kDa

false

Exposure time: 60s

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

10C2

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, ICC

applications

Immunogen

Recombinant Fragment Protein within Human NES aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P48681

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p>1/1000 - 1/5000 using chemiluminescent detection. Predicted molecular weight: 176 kDa.</p>" } } }

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Product details

What is this antibody validated in?
Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (ab22035) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Human samples.

What is the molecular weight of Nestin?
Anti-Nestin [10C2] - Neural Stem Cell Marker (ab22035) specifically detects a band for Nestin (UniProt: P48681) at a molecular weight of 176kDa.

Trusted by the scientific community
Anti-Nestin [10C2] - Neural Stem Cell Marker (ab22035) was first used in a scientific publication in 2005 and has been cited over 150 times in peer-reviewed journals.

Reviewed by scientists
Anti-Nestin [10C2] - Neural Stem Cell Marker (ab22035) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nestin also known as NES is an intermediate filament protein with a molecular weight of about 220 kDa. Nestin features prominently in cells related to the nervous system including neural stem cells and it serves as a structural component in these cells. Nestin also appears in substantial amounts during the development of the nervous system and is important for cell structural organization. It is often used as a Nestin marker in research particularly with Nestin antibodies for identifying the presence of neural stem cells and tracking their developmental course. Researchers frequently utilize techniques like Nestin IHC (immunohistochemistry) and Nestin western blot to stain and detect Nestin allowing detailed investigations into its expression in various tissues.

Biological function summary

Nestin plays a significant role in the dynamic remodeling of the cytoskeleton. It does not act as a solitary element but forms part of a larger protein complex involved in maintaining and organizing cellular structure. This property grants cells flexibility in shape and resilience during the rapid changes in development and specialization. The role of Nestin in the cellular architecture allows it to contribute to processes such as cell migration and proliferation critical during tissue development and repair.

Pathways

Nestin integrates into networks that regulate stem cell behavior and neurogenesis. It tightly interacts within the pathways involving cell cycle regulation and differentiation. For example the Wnt and Notch signaling pathways modulate its expression to influence neural stem cell fate and patterning during development. Protein interactions between Nestin and other cytoskeletal elements like vimentin facilitate its roles in these pathways adapting the cellular environment for specific developmental activities.

Nestin associates with various pathological conditions including brain tumors and muscular dystrophies. Its expression is aberrant in many forms of gliomas where it often signifies a higher grade of malignancy. Within these tumors Nestin connects to oncogenic signaling pathways involving proteins such as the epidermal growth factor receptor (EGFR). Moreover in muscular dystrophies Nestin's expression in regenerating muscle fibers illustrates its involvement in cellular repair mechanisms potentially interacting with dystrophin a protein important for muscle integrity. Research on anti-Nestin antibodies continues to clarify its role in these diseases potentially offering diagnostic or therapeutic insights.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).

See full target information NES

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Publications (204)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:27045 PubMed40715563

2025

Curcumin exerts neuroprotective effects on proliferation of neural stem cells in vitro and APP/PS1 mouse model in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Juanli Chen,Chuanfei Wei,Yingchao Zhang,Qingfa Chen,Xuemei Li,Wei Zhang,Xiaoping Zhang,Fabin Han

JCI insight 10: PubMed40693462

2025

N6-methyladenosine (m6A) dysregulation contributes to network excitability in temporal lobe epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Justine Mathoux,Marc-Michel Wilson,Sujithra Srinivas,Gabrielle Litovskich,Leticia Villalba Benito,Cindy Tran,Jaideep Kesavan,Aileen Harnett,Theresa Auer,Amaya Sanz-Rodriguez,Mohammad Kh A E Alkhayyat,Mairéad Sullivan,Zining Liu,Yifan Huang,Austin Lacey,Norman Delanty,Jane Cryan,Francesca M Brett,Michael A Farrell,Donncha F O'Brien,Pablo M Casillas-Espinosa,Eva M Jimenez-Mateos,Jeffrey C Glennon,Mary Canavan,David C Henshall,Gary P Brennan

EBioMedicine 118:105833 PubMed40580577

2025

Comprehensive immune ageing reveals TREM2/TIM3 myeloid cells drive brain immune evasion.

Applications

Unspecified application

Species

Unspecified reactive species

Berta Segura-Collar,Lucia Mondejar-Ruescas,Denisse Alcivar-López,María Garranzo-Asensio,Pablo Mata-Martinez,Ramón Garcia-Escudero,Aurelio Hernández-Laín,Juan M Sepúlveda,Ángel Pérez Nuñez,Jesús Avila,Pilar Sánchez-Gómez,Ricardo Gargini

Stem cells translational medicine 14: PubMed40213860

2025

Reprogramming of skin fibroblasts by 3D spheroid culture promotes peripheral nerve regeneration via the ID3/semaphorin7a pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Tan,Zhou Zhang,Xiaohui Cao,Langfan Qu,Yinchun Xiong,Huijuan Li,Yu Wang,Zelin Chen,Chunmeng Shi

Communications biology 8:410 PubMed40069408

2025

Mitochondrial dysfunction drives a neuronal exhaustion phenotype in methylmalonic aciduria.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew C S Denley,Monique S Straub,Giulio Marcionelli,Miriam A Güra,David Penton,Igor Delvendahl,Martin Poms,Beata Vekeriotaite,Sarah Cherkaoui,Federica Conte,Ferdinand von Meyenn,D Sean Froese,Matthias R Baumgartner

Pediatric research 98:1155-1165 PubMed39939521

2025

Spatiotemporal expression pattern of dyslexia susceptibility 1 candidate 1 (DYX1C1) during rat cerebral cortex development.

Applications

Unspecified application

Species

Unspecified reactive species

Kazumasa Zensho,Ikuko Miyazaki,Aika Isse,Ichika Misawa,Kaori Masai,Makio Oka,Hirokazu Tsukahara,Masato Asanuma

Pharmaceuticals (Basel, Switzerland) 17: PubMed39770529

2025

A Repurposed Drug Selection Pipeline to Identify CNS-Penetrant Drug Candidates for Glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ioannis Ntafoulis,Stijn L W Koolen,Olaf van Tellingen,Chelsea W J den Hollander,Hendrika Sabel-Goedknegt,Stephanie Dijkhuizen,Joost Haeck,Thom G A Reuvers,Peter de Bruijn,Thierry P P van den Bosch,Vera van Dis,Zhenyu Gao,Clemens M F Dirven,Sieger Leenstra,Martine L M Lamfers

Life science alliance 8: PubMed39741006

2025

NSD3 protein methylation and stabilization transforms human ES cells into variant state.

Applications

Unspecified application

Species

Unspecified reactive species

Vignesh K Krishnamoorthy,Fariha Hamdani,Pooja Shukla,Radhika Arasala Rao,Shaikh Anaitullah,Kriti Kestur Biligiri,Rajashekar Varma Kadumuri,Purushotham Reddy Pothula,Sreenivas Chavali,Shravanti Rampalli

Stem cell research & therapy 15:465 PubMed39639398

2024

Using magnetic resonance relaxometry to evaluate the safety and quality of induced pluripotent stem cell-derived spinal cord progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jerome Tan,Jiahui Chen,Daniel Roxby,Wai Hon Chooi,Tan Dai Nguyen,Shi Yan Ng,Jongyoon Han,Sing Yian Chew

Cells 13: PubMed39513909

2024

ALDH1A3 Contributes to Radiation-Induced Inhibition of Self-Renewal and Promotes Proliferative Activity of p53-Deficient Glioblastoma Stem Cells at the Onset of Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Müller,Bogdan Lyubarskyy,Jurij Tchoumakov,Maike Wagner,Bettina Sprang,Florian Ringel,Ella L Kim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nestin antibody [10C2] - Neural Stem Cell Marker

Immunocytochemistry - Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (AB22035)

Immunocytochemistry - Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (AB22035)

Immunocytochemistry - Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (AB22035)

Western blot - Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (AB22035)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (204)

Product promise