AB254048

Anti-Nestin antibody [4D11] - Neural Stem Cell Marker

Name: Anti-Nestin antibody [4D11] - Neural Stem Cell Marker (ab254048) | Abcam
Brand: Abcam Limited
SKU: AB254048
Price: 525 USD
Availability: InStock

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(7 Publications)

Mouse Monoclonal Nestin antibody. Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NES.

View Alternative Names

Nbla00170, NES, Nestin

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [4D11] - Neural Stem Cell Marker (AB254048)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [4D11] - Neural Stem Cell Marker (AB254048)

Immunostaining of cultured neonatal rat neurons and glia using ab254048 at 1/500 dilution showing Nestin labeling in red and vimentin in green.

Supplier Data

Western blot - Anti-Nestin antibody [4D11] - Neural Stem Cell Marker (AB254048)

All lanes:

Western blot - Anti-Nestin antibody [4D11] - Neural Stem Cell Marker (ab254048) at 1/2000 dilution

All lanes:

Rat brain lysate

Predicted band size: 177 kDa

false

Unconjugated

Anti-6X His tag® antibody [4D11]
Unconjugated

Anti-ATP5D antibody [4D11]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4D11

Isotype

IgG1

Carrier free

Reacts with

Rat

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NES. The exact immunogen used to generate this antibody is proprietary information.

P48681

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from TCS.

Storage buffer

Preservative: 0.0325% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nestin also known as NES is an intermediate filament protein with a molecular weight of about 220 kDa. Nestin features prominently in cells related to the nervous system including neural stem cells and it serves as a structural component in these cells. Nestin also appears in substantial amounts during the development of the nervous system and is important for cell structural organization. It is often used as a Nestin marker in research particularly with Nestin antibodies for identifying the presence of neural stem cells and tracking their developmental course. Researchers frequently utilize techniques like Nestin IHC (immunohistochemistry) and Nestin western blot to stain and detect Nestin allowing detailed investigations into its expression in various tissues.

Biological function summary

Nestin plays a significant role in the dynamic remodeling of the cytoskeleton. It does not act as a solitary element but forms part of a larger protein complex involved in maintaining and organizing cellular structure. This property grants cells flexibility in shape and resilience during the rapid changes in development and specialization. The role of Nestin in the cellular architecture allows it to contribute to processes such as cell migration and proliferation critical during tissue development and repair.

Pathways

Nestin integrates into networks that regulate stem cell behavior and neurogenesis. It tightly interacts within the pathways involving cell cycle regulation and differentiation. For example the Wnt and Notch signaling pathways modulate its expression to influence neural stem cell fate and patterning during development. Protein interactions between Nestin and other cytoskeletal elements like vimentin facilitate its roles in these pathways adapting the cellular environment for specific developmental activities.

Nestin associates with various pathological conditions including brain tumors and muscular dystrophies. Its expression is aberrant in many forms of gliomas where it often signifies a higher grade of malignancy. Within these tumors Nestin connects to oncogenic signaling pathways involving proteins such as the epidermal growth factor receptor (EGFR). Moreover in muscular dystrophies Nestin's expression in regenerating muscle fibers illustrates its involvement in cellular repair mechanisms potentially interacting with dystrophin a protein important for muscle integrity. Research on anti-Nestin antibodies continues to clarify its role in these diseases potentially offering diagnostic or therapeutic insights.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).

See full target information NES

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Brain sciences 15: PubMed40149758

2025

The pCREB/BDNF Pathway in the Hippocampus Is Involved in the Therapeutic Effect of Selective 5-HT Reuptake Inhibitors in Adult Male Rats Exposed to Blast Traumatic Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolin Fan,Hong Wang,Xiaoqiang Lv,Qi Wang,Boya Yu,Xiao Li,Liang Li,Yuhao Zhang,Ning Ma,Qing Lu,Airong Qian,Junhong Gao

FASEB bioAdvances 7:e70000 PubMed40060945

2025

Inhibition of the Hippo pathway by verteporfin reduces the proliferation and stemness of rat hair follicle neural crest stem cells under hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Weihai Liu,Suhua Chen,Xin Chen,Lixiang Xue,Yan Wang,Jun Yang

Molecular psychiatry 30:2994-3007 PubMed39900676

2025

Maternal immune activation imprints translational dysregulation and differential MAP2 phosphorylation in descendant neural stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra M Martín-Guerrero,María Martín-Estebané,Antonio J Lara Ordóñez,Miguel Cánovas,David Martín-Oliva,Javier González-Maeso,Pedro R Cutillas,Juan F López-Giménez

Neural regeneration research 19:1772-1780 PubMed38103244

2023

High-frequency repetitive transcranial magnetic stimulation promotes neural stem cell proliferation after ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Luo,Yuan Feng,Zhongqiu Hong,Mingyu Yin,Haiqing Zheng,Liying Zhang,Xiquan Hu

Cell death discovery 8:350 PubMed35927251

2022

M2-like tumor-associated macrophages transmit exosomal miR-27b-3p and maintain glioblastoma stem-like cell properties.

Applications

Unspecified application

Species

Unspecified reactive species

Guifang Zhao,Lijuan Ding,Hongquan Yu,Weiyao Wang,Huan Wang,Yao Hu,Lingsha Qin,Guangce Deng,Buqing Xie,Guofeng Li,Ling Qi

Frontiers in molecular neuroscience 15:829642 PubMed35283722

2022

Shikonin Attenuates Cochlear Spiral Ganglion Neuron Degeneration by Activating Nrf2-ARE Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongjie Du,Xuanchen Zhou,Lei Shi,Ming Xia,Yajie Wang,Na Guo,Houyang Hu,Pan Zhang,Huiming Yang,Fangyuan Zhu,Zhenxiao Teng,Chengcheng Liu,Miaoqing Zhao

NPJ Regenerative medicine 6:31 PubMed34078912

2021

Preclinical assessment on neuronal regeneration in the injury-related microenvironment of graphene-based scaffolds.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Qian,Xu Wang,Jialin Song,Wei Chen,Shuai Chen,Yi Jin,Yuanming Ouyang,Wei-En Yuan,Cunyi Fan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nestin antibody [4D11] - Neural Stem Cell Marker

Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [4D11] - Neural Stem Cell Marker (AB254048)

Western blot - Anti-Nestin antibody [4D11] - Neural Stem Cell Marker (AB254048)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise