AB221660

Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker

Name: Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (ab221660) | Abcam
Brand: Abcam Limited
SKU: AB221660
Price: 345 USD
Availability: InStock
Rating: 1 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(35 Publications)

Anti-Nestin antibody [EPR22023] (ab221660) is a rabbit monoclonal antibody detecting Nestin in Western Blot, IHC-P, IHC-Fr. Suitable for Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications

View Alternative Names

Nestin, Nes

6 Images

Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Nestin with ab221660 at 1/100 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Cytoplasmic staining in glomeruli of mouse kidney (PMID : 17210924) is observed. Counter stained with DAPI (blue).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit at a 1/1000 dilution.

Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling Nestin with ab221660 at 1/100 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Cytoplasmic staining in glomeruli of rat kidney (PMID : 17210924) is observed. Counter stained with DAPI (blue).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit at a 1/1000 dilution.

Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Nestin with ab221660 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining of glomerulus and stromal cells in mouse kidney (PMID : 17210924) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Nestin with ab221660 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in mouse lung (PMID : 17210924) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

Immunohistochemical analysis of paraffin-embedded mouse embryonic day 14.5 cerebrum tissue labeling Nestin with ab221660 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on cerebrum of embryonic day 14.5 mouse (PMID : 16728475) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

Blocking/diluting buffer and concentration : 5% NFDM/TBST.

Exposure times : Lane 1 : 26 seconds; Lane 2 : 81 seconds; Lane 3 : 15 seconds.

The molecular mass observed is consistent with what has been described in the literature (PMID : 23319587, PMID : 23407796).

All lanes:

Western blot - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (ab221660) at 1/1000 dilution

Lane 1:

Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Mouse P0 brain lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 177 kDa

false

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker
Carrier free

Anti-Nestin antibody [EPR22023] - BSA and Azide free
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22023

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P, WB, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "Perform heat-mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20)", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "Perform heat-mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20)", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Nestin antibody [EPR22023] (ab221660) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Mouse, Rat samples.

What is the molecular weight of Nestin?
Anti-Nestin [EPR22023] (ab221660) specifically detects a band for Nestin (UniProt: Q6P5H2) at a molecular weight of 177kDa.

Trusted by the scientific community
Anti-Nestin [EPR22023] (ab221660) was first used in a scientific publication in 2018 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nestin also known as NES is an intermediate filament protein with a molecular weight of about 220 kDa. Nestin features prominently in cells related to the nervous system including neural stem cells and it serves as a structural component in these cells. Nestin also appears in substantial amounts during the development of the nervous system and is important for cell structural organization. It is often used as a Nestin marker in research particularly with Nestin antibodies for identifying the presence of neural stem cells and tracking their developmental course. Researchers frequently utilize techniques like Nestin IHC (immunohistochemistry) and Nestin western blot to stain and detect Nestin allowing detailed investigations into its expression in various tissues.

Biological function summary

Nestin plays a significant role in the dynamic remodeling of the cytoskeleton. It does not act as a solitary element but forms part of a larger protein complex involved in maintaining and organizing cellular structure. This property grants cells flexibility in shape and resilience during the rapid changes in development and specialization. The role of Nestin in the cellular architecture allows it to contribute to processes such as cell migration and proliferation critical during tissue development and repair.

Pathways

Nestin integrates into networks that regulate stem cell behavior and neurogenesis. It tightly interacts within the pathways involving cell cycle regulation and differentiation. For example the Wnt and Notch signaling pathways modulate its expression to influence neural stem cell fate and patterning during development. Protein interactions between Nestin and other cytoskeletal elements like vimentin facilitate its roles in these pathways adapting the cellular environment for specific developmental activities.

Nestin associates with various pathological conditions including brain tumors and muscular dystrophies. Its expression is aberrant in many forms of gliomas where it often signifies a higher grade of malignancy. Within these tumors Nestin connects to oncogenic signaling pathways involving proteins such as the epidermal growth factor receptor (EGFR). Moreover in muscular dystrophies Nestin's expression in regenerating muscle fibers illustrates its involvement in cellular repair mechanisms potentially interacting with dystrophin a protein important for muscle integrity. Research on anti-Nestin antibodies continues to clarify its role in these diseases potentially offering diagnostic or therapeutic insights.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division (By similarity). Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

See full target information Nes

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Publications (35)

Recent publications for all applications. Explore the full list and refine your search

Biomaterials research 29:0256 PubMed40980288

2025

Decreasing Lamin A Triggers Cell Fate Transitions through Heterochromatin-Nuclear Periphery Detethering.

Applications

Unspecified application

Species

Unspecified reactive species

Lijuan Sun,Yafan Xie,Zhaoyan Zuo,Jian Liu,Jiaqi Yang,Iqra Ali,Qin Peng,Juhui Qiu

Acta neuropathologica communications 13:158 PubMed40684195

2025

Unraveling the development of cutaneous neurofibromas in neurofibromatosis type 1.

Applications

Unspecified application

Species

Unspecified reactive species

Pernelle Pulh,Fanny Coulpier,Audrey Onfroy,Layna Oubrou,Wanzhen Zhang,Léa Toledano,Elie Abou Zougheib,Laura Fertitta,Pierre Wolkenstein,Piotr Topilko

Cell reports. Medicine 6:102052 PubMed40239632

2025

Catalytic neural stem cell exosomes for multi-stage targeting and synergistical therapy of retinal ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Weiqiang Yang,Xiaojun Wang,Diwei Zheng,Jing Feng,Wenjun Kong,Yue Li,Guanghui Ma,Wei Wei,Yong Tao

Translational stroke research 16:1577-1593 PubMed39853651

2025

Neuroprotective Effects, Mechanisms of Action and Therapeutic Potential of the Kv7/KCNQ Channel Opener QO-83 in Ischemic Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Huiran Zhang,Yanfei Tian,Yan Zhang,Yan Wang,Jinlong Qi,Xiangyu Wang,Yi Yuan,Rong Chen,Yupeng Zhao,Chang Liu,Najing Zhou,Lanxin Liu,Han Hao,Xiaona Du,Hailin Zhang

Journal of advanced research 74:429-442 PubMed39353531

2024

Gastrodin attenuates high fructose-induced sweet taste preference decrease by inhibiting hippocampal neural stem cell ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chuan-Feng Tang,Hong Ding,Ya-Qian Wu,Zi-An Miao,Zi-Xuan Wang,Wen-Xuan Wang,Ying Pan,Ling-Dong Kong

Bone research 12:34 PubMed38816384

2024

Lumbar instability remodels cartilage endplate to induce intervertebral disc degeneration by recruiting osteoclasts via Hippo-CCL3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hanwen Li,Yingchuang Tang,Zixiang Liu,Kangwu Chen,Kai Zhang,Sihan Hu,Chun Pan,Huilin Yang,Bin Li,Hao Chen

Neural regeneration research 20:557-573 PubMed38819067

2024

Mutual regulation of microglia and astrocytes after Gas6 inhibits spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jiewen Chen,Xiaolin Zeng,Le Wang,Wenwu Zhang,Gang Li,Xing Cheng,Peiqiang Su,Yong Wan,Xiang Li

BioMed research international 2024:2973407 PubMed38449509

2024

Identifying G6PC3 as a Potential Key Molecule in Hypoxic Glucose Metabolism of Glioblastoma Derived from the Depiction of F-Fluoromisonidazole and F-Fluorodeoxyglucose Positron Emission Tomography.

Applications

Unspecified application

Species

Unspecified reactive species

Michinari Okamoto,Shigeru Yamaguchi,Ryosuke Sawaya,Sumire Echizenya,Yukitomo Ishi,Sadahiro Kaneko,Hiroaki Motegi,Takuya Toyonaga,Kenji Hirata,Miki Fujimura

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed38383496

2024

SMARCA4 Loss and Mutated β-Catenin Induce Proliferative Lesions in the Murine Embryonic Cerebellum.

Applications

Unspecified application

Species

Unspecified reactive species

Carolin Göbel,Melanie Schoof,Dörthe Holdhof,Michael Spohn,Ulrich Schüller

Journal of nanobiotechnology 22:66 PubMed38368393

2024

Exosome lncRNA IFNG-AS1 derived from mesenchymal stem cells of human adipose ameliorates neurogenesis and ASD-like behavior in BTBR mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Fu,Yuan-Lin Zhang,Rong-Qi Liu,Meng-Meng Xu,Jun-Ling Xie,Xing-Liao Zhang,Guang-Ming Xie,Yao-Ting Han,Xin-Min Zhang,Wan-Ting Zhang,Jing Zhang,Jun Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker

Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

Western blot - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (35)

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