Anti-Nestin antibody [EPR22023] (ab221660) is a rabbit monoclonal antibody that is used to detect Nestin in Western Blot, IHC-P, IHC-Fr. Suitable for Mouse, Rat samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 20 publications
Images
![Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-epr22023-neural-stem-cell-marker-ab221660--immunohistochemistry-frozen-sections-img17953.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)")
![Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-epr22023-neural-stem-cell-marker-ab221660--immunohistochemistry-frozen-sections-img100787.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)")
![Western blot - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-epr22023-neural-stem-cell-marker-ab221660--western-blot-img10196.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-epr22023-neural-stem-cell-marker-ab221660--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117620.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-epr22023-neural-stem-cell-marker-ab221660--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107836.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)")
Publications
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- Nature communications 14:77172023Mouse models of pediatric high-grade gliomas with MYCN amplification reveal intratumoral heterogeneity and lineage signatures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMelanie Schoof,Shweta Godbole,Thomas K Albert,Matthias Dottermusch,Carolin Walter,Annika Ballast,Nan Qin,Marlena Baca Olivera,Carolin Göbel,Sina Neyazi,Dörthe Holdhof,Catena Kresbach,Levke-Sophie Peter,Gefion Dorothea Epplen,Vanessa Thaden,Michael Spohn,Mirjam Blattner-Johnson,Franziska Modemann,Martin Mynarek,Stefan Rutkowski,Martin Sill,Julian Varghese,Ann-Kristin Afflerbach,Alicia Eckhardt,Daniel Münter,Archana Verma,Nina Struve,David T W Jones,Marc Remke,Julia E Neumann,Kornelius Kerl,Ulrich SchüllerPubMed 38001143
- Acta neuropathologica communications 11:1742023MYC overexpression and SMARCA4 loss cooperate to drive medulloblastoma formation in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarolin Göbel,Shweta Godbole,Melanie Schoof,Dörthe Holdhof,Catena Kresbach,Carolin Loose,Julia Neumann,Ulrich SchüllerPubMed 37919824
- Nature communications 14:44672023Circular RNA encoded MET variant promotes glioblastoma tumorigenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Zhong,Xujia Wu,Yixin Gao,Junju Chen,Maolei Zhang,Huangkai Zhou,Jia Yang,Feizhe Xiao,Xuesong Yang,Nunu Huang,Haoyue Qi,Xiuxing Wang,Fan Bai,Yu Shi,Nu ZhangPubMed 37491377
- Advanced healthcare materials 12:e23011512023Copper Ion-Modified Germanium Phosphorus Nanosheets Integrated with an Electroactive and Biodegradable Hydrogel for Neuro-Vascularized Bone Regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Xu,Chao Xu,Kun Yang,Liang Ma,Gaocai Li,Yunsong Shi,Xiaobo Feng,Lei Tan,Deyu Duan,Zhiqiang Luo,Cao YangPubMed 37421228
- Acta pharmaceutica Sinica. B 13:2017-20382023Neuron stem cell NLRP6 sustains hippocampal neurogenesis to resist stress-induced depression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuanfeng Tang,Qiaona Wang,Jingyan Shen,Congying Wang,Hong Ding,Shiyu Wen,Fan Yang,Ruiqing Jiao,Xingxin Wu,Jianmei Li,Lingdong KongPubMed 37250149
- Frontiers in immunology 14:11331772023Protective anti-tumor vaccination against glioblastoma expressing the MHC class II transactivator CIITA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFabrizio Celesti,Andrea Gatta,Mariam Shallak,Anna Maria Chiaravalli,Michele Cerati,Fausto Sessa,Roberto S Accolla,Greta ForlaniPubMed 36993983
- International journal of oncology 61:2022Neural stem cell‑derived exosomes transfer miR‑124‑3p into cells to inhibit glioma growth by targeting FLOT2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng Qian,You Wang,Yunxiang Ji,Danmin Chen,Chuanfang Wang,Guilong Zhang,Yezhong WangPubMed 35929514
- Cancers 14:2022Exploratory Analysis of Serial F-fluciclovine PET-CT and Multiparametric MRI during Chemoradiation for Glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKavi Fatania,Russell Frood,Marcus Tyyger,Garry McDermott,Sharon Fernandez,Gary C Shaw,Marjorie Boissinot,Daniela Salvatore,Luisa Ottobrini,Irvin Teh,John Wright,Marc A Bailey,Joanna Koch-Paszkowski,Jurgen E Schneider,David L Buckley,Louise Murray,Andrew Scarsbrook,Susan C Short,Stuart CurriePubMed 35884545
- Neurochemistry international 159:1053872022Role of hyperpolarization-activated cyclic nucleotide-gated channel HCN2 in embryonic neural stem cell proliferation and differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTommy Nordström,Leif C Andersson,Karl E O ÅkermanPubMed 35835292
- Nutrients 14:2022Short-Chain Fatty Acids Ameliorate Depressive-like Behaviors of High Fructose-Fed Mice by Rescuing Hippocampal Neurogenesis Decline and Blood-Brain Barrier Damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuan-Feng Tang,Cong-Ying Wang,Jun-Han Wang,Qiao-Na Wang,Shen-Jie Li,Hai-Ou Wang,Feng Zhou,Jian-Mei LiPubMed 35565849
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| WB | IHC-Fr | IHC-P | |
|---|---|---|---|
| Mouse | Tested | Tested | Tested |
| Rat | Expected | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20) |
Species Rat | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20) |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division (By similarity). Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Alternative names
Nestin, Nes
Recommended products
Anti-Nestin antibody [EPR22023] (ab221660) is a rabbit monoclonal antibody that is used to detect Nestin in Western Blot, IHC-P, IHC-Fr. Suitable for Mouse, Rat samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 20 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR22023
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Nestin antibody [EPR22023] (ab221660) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Mouse, Rat samples.
What is the molecular weight of Nestin?
Anti-Nestin [EPR22023] (ab221660) specifically detects a band for Nestin (UniProt: Q6P5H2) at a molecular weight of 177kDa.
Trusted by the scientific community
Anti-Nestin [EPR22023] (ab221660) was first used in a scientific publication in 2018 and has been cited over 20 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nestin also known as NES is an intermediate filament protein with a molecular weight of about 220 kDa. Nestin features prominently in cells related to the nervous system including neural stem cells and it serves as a structural component in these cells. Nestin also appears in substantial amounts during the development of the nervous system and is important for cell structural organization. It is often used as a Nestin marker in research particularly with Nestin antibodies for identifying the presence of neural stem cells and tracking their developmental course. Researchers frequently utilize techniques like Nestin IHC (immunohistochemistry) and Nestin western blot to stain and detect Nestin allowing detailed investigations into its expression in various tissues.
- Biological function summary
Nestin plays a significant role in the dynamic remodeling of the cytoskeleton. It does not act as a solitary element but forms part of a larger protein complex involved in maintaining and organizing cellular structure. This property grants cells flexibility in shape and resilience during the rapid changes in development and specialization. The role of Nestin in the cellular architecture allows it to contribute to processes such as cell migration and proliferation critical during tissue development and repair.
- Pathways
Nestin integrates into networks that regulate stem cell behavior and neurogenesis. It tightly interacts within the pathways involving cell cycle regulation and differentiation. For example the Wnt and Notch signaling pathways modulate its expression to influence neural stem cell fate and patterning during development. Protein interactions between Nestin and other cytoskeletal elements like vimentin facilitate its roles in these pathways adapting the cellular environment for specific developmental activities.
- Associated diseases and disorders
Nestin associates with various pathological conditions including brain tumors and muscular dystrophies. Its expression is aberrant in many forms of gliomas where it often signifies a higher grade of malignancy. Within these tumors Nestin connects to oncogenic signaling pathways involving proteins such as the epidermal growth factor receptor (EGFR). Moreover in muscular dystrophies Nestin's expression in regenerating muscle fibers illustrates its involvement in cellular repair mechanisms potentially interacting with dystrophin a protein important for muscle integrity. Research on anti-Nestin antibodies continues to clarify its role in these diseases potentially offering diagnostic or therapeutic insights.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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6 product images
![Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (ab221660), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-epr22023-neural-stem-cell-marker-ab221660--immunohistochemistry-frozen-sections-img17953.jpg "Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (ab221660)")
Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (ab221660)
Nestin Immunohistochemistry (Frozen sections) staining using rabbit Anti-Nestin antibody
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Nestin with ab221660 at 1/100 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Cytoplasmic staining in glomeruli of mouse kidney (PMID: 17210924) is observed. Counter stained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit at a 1/1000 dilution.Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
![Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (ab221660), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-epr22023-neural-stem-cell-marker-ab221660--immunohistochemistry-frozen-sections-img100787.jpg "Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (ab221660)")
Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (ab221660)
Nestin Immunohistochemistry (Frozen sections) staining using rabbit Anti-Nestin antibody
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling Nestin with ab221660 at 1/100 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Cytoplasmic staining in glomeruli of rat kidney (PMID: 17210924) is observed. Counter stained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit at a 1/1000 dilution.Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
![Western blot - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (ab221660), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-epr22023-neural-stem-cell-marker-ab221660--western-blot-img10196.jpg "Western blot - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (ab221660)")
Western blot - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (ab221660)
Nestin Western blot staining using rabbit Anti-Nestin antibody
Blocking/diluting buffer and concentration: 5% NFDM/TBST.Exposure times: Lane 1: 26 seconds; Lane 2: 81 seconds; Lane 3: 15 seconds.
The molecular mass observed is consistent with what has been described in the literature (PMID: 23319587, PMID: 23407796).
All lanes: Western blot - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (ab221660) at 1/1000 dilution
Lane 1: Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg
Lane 2: Mouse heart lysate at 10 µg
Lane 3: Mouse P0 brain lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 177 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (ab221660), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-epr22023-neural-stem-cell-marker-ab221660--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117620.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (ab221660)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (ab221660)
Nestin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Nestin antibody
Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Nestin with ab221660 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in mouse lung (PMID: 17210924) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (ab221660), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-epr22023-neural-stem-cell-marker-ab221660--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107836.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (ab221660)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (ab221660)
Nestin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Nestin antibody
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Nestin with ab221660 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining of glomerulus and stromal cells in mouse kidney (PMID: 17210924) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (ab221660), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-epr22023-neural-stem-cell-marker-ab221660--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116318.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (ab221660)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (ab221660)
Nestin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Nestin antibody
Immunohistochemical analysis of paraffin-embedded mouse embryonic day 14.5 cerebrum tissue labeling Nestin with ab221660 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on cerebrum of embryonic day 14.5 mouse (PMID: 16728475) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (ab221660), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-epr22023-neural-stem-cell-marker-ab221660--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116318.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (ab221660)")
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