AB313787

Anti-Nestin antibody [EPR27207-53] - Neural Stem Cell Marker

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal Nestin antibody. Suitable for IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

Nestin, Nes

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR27207-53] - Neural Stem Cell Marker (AB313787)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR27207-53] - Neural Stem Cell Marker (AB313787)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Nestin with ab313787 at 1/2000 (0.257 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human placenta.The section was incubated with ab313787 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR27207-53] - Neural Stem Cell Marker (AB313787)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Nestin with ab313787 at 1/2000 (0.257 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on glomerulus and peritubular cells of human kidney(PMID : 20649878).The section was incubated with ab313787 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR27207-53] - Neural Stem Cell Marker (AB313787)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Nestin with ab313787 at 1/2000 (0.257 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on glomerulus and peritubular cells of mouse kidney.The section was incubated with ab313787 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR27207-53] - Neural Stem Cell Marker (AB313787)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Nestin with ab313787 at 1/2000 (0.257 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on glomerulus and peritubular cells of rat kidney.The section was incubated with ab313787 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR27207-53] - Neural Stem Cell Marker (AB313787)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Nestin with ab313787 at 1/2000 (0.257 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on endothelium of mouse cerebrum.The section was incubated with ab313787 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [EPR27207-53] - Neural Stem Cell Marker (AB313787)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [EPR27207-53] - Neural Stem Cell Marker (AB313787)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized C2C12 (mouse myoblast) cells labelling Nestin with ab313787 at 1/50 (10.28 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in C2C12 cell line.Negative control : Beta-TC-6.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Nestin antibody [EPR27207-53] - Neural Stem Cell Marker
Carrier free

Anti-Nestin antibody [EPR27207-53] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27207-53

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Unsuitable for rat ICC/IF.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nestin also known as NES is an intermediate filament protein with a molecular weight of about 220 kDa. Nestin features prominently in cells related to the nervous system including neural stem cells and it serves as a structural component in these cells. Nestin also appears in substantial amounts during the development of the nervous system and is important for cell structural organization. It is often used as a Nestin marker in research particularly with Nestin antibodies for identifying the presence of neural stem cells and tracking their developmental course. Researchers frequently utilize techniques like Nestin IHC (immunohistochemistry) and Nestin western blot to stain and detect Nestin allowing detailed investigations into its expression in various tissues.

Biological function summary

Nestin plays a significant role in the dynamic remodeling of the cytoskeleton. It does not act as a solitary element but forms part of a larger protein complex involved in maintaining and organizing cellular structure. This property grants cells flexibility in shape and resilience during the rapid changes in development and specialization. The role of Nestin in the cellular architecture allows it to contribute to processes such as cell migration and proliferation critical during tissue development and repair.

Pathways

Nestin integrates into networks that regulate stem cell behavior and neurogenesis. It tightly interacts within the pathways involving cell cycle regulation and differentiation. For example the Wnt and Notch signaling pathways modulate its expression to influence neural stem cell fate and patterning during development. Protein interactions between Nestin and other cytoskeletal elements like vimentin facilitate its roles in these pathways adapting the cellular environment for specific developmental activities.

Nestin associates with various pathological conditions including brain tumors and muscular dystrophies. Its expression is aberrant in many forms of gliomas where it often signifies a higher grade of malignancy. Within these tumors Nestin connects to oncogenic signaling pathways involving proteins such as the epidermal growth factor receptor (EGFR). Moreover in muscular dystrophies Nestin's expression in regenerating muscle fibers illustrates its involvement in cellular repair mechanisms potentially interacting with dystrophin a protein important for muscle integrity. Research on anti-Nestin antibodies continues to clarify its role in these diseases potentially offering diagnostic or therapeutic insights.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division (By similarity). Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

See full target information Nes

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

IBRO neuroscience reports 18:627-637 PubMed40584426

2025

Neural stem cell changes and spatial distribution of AQP4 expression in a fetal goat model of obstructive hydrocephalus.

Applications

Unspecified application

Species

Unspecified reactive species

Xinting Ji,Qin Zhang,Yuantao Yang,Yan Li,Lihong Li,Shibin Lin,Qi Dong,Zhenling Wan,Julan Wu,Jiadong Bao,Xiaoman Su,Pengzhi Chen,Chenyuan Liu,Weian Song,Zechen Wang,Kang Feng,Xiaoying Li,Shaojiang Zheng,Shuo Gu

Drug design, development and therapy 18:4779-4797 PubMed39494153

2024

Bu Shen Huo Xue Formula Provides Neuroprotection Against Spinal Cord Injury by Inhibiting Oxidative Stress by Activating the Nrf2 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Luo,Yonghui Hou,Jiheng Zhan,Yu Hou,Zenglu Wang,Xing Li,Lili Sui,Shudong Chen,Dingkun Lin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nestin antibody [EPR27207-53] - Neural Stem Cell Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR27207-53] - Neural Stem Cell Marker (AB313787)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR27207-53] - Neural Stem Cell Marker (AB313787)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR27207-53] - Neural Stem Cell Marker (AB313787)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR27207-53] - Neural Stem Cell Marker (AB313787)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR27207-53] - Neural Stem Cell Marker (AB313787)

Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [EPR27207-53] - Neural Stem Cell Marker (AB313787)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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