Chicken Polyclonal Nestin antibody. Suitable for ICC/IF, WB, IHC-P and reacts with Mouse samples. Cited in 31 publications. Immunogen corresponding to Synthetic Peptide within Mouse Nes conjugated to Keyhole Limpet Haemocyanin.
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 99% PBS
ICC/IF | WB | IHC-P | |
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Mouse | Tested | Tested | Tested |
Species | Dilution info | Notes |
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Species Mouse | Dilution info 1/1000 - 1/2000 | Notes - |
Species | Dilution info | Notes |
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Species Mouse | Dilution info 1/20000 - 1/50000 | Notes - |
Species | Dilution info | Notes |
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Species Mouse | Dilution info 1/1000 - 1/2000 | Notes - |
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Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division (By similarity). Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Nestin, Nes
Chicken Polyclonal Nestin antibody. Suitable for ICC/IF, WB, IHC-P and reacts with Mouse samples. Cited in 31 publications. Immunogen corresponding to Synthetic Peptide within Mouse Nes conjugated to Keyhole Limpet Haemocyanin.
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 99% PBS
IgY fractions were purified from the yolks and IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 300 µg/ml, and the preparation filter-sterilized.
Nestin also known as NES is an intermediate filament protein with a molecular weight of about 220 kDa. Nestin features prominently in cells related to the nervous system including neural stem cells and it serves as a structural component in these cells. Nestin also appears in substantial amounts during the development of the nervous system and is important for cell structural organization. It is often used as a Nestin marker in research particularly with Nestin antibodies for identifying the presence of neural stem cells and tracking their developmental course. Researchers frequently utilize techniques like Nestin IHC (immunohistochemistry) and Nestin western blot to stain and detect Nestin allowing detailed investigations into its expression in various tissues.
Nestin plays a significant role in the dynamic remodeling of the cytoskeleton. It does not act as a solitary element but forms part of a larger protein complex involved in maintaining and organizing cellular structure. This property grants cells flexibility in shape and resilience during the rapid changes in development and specialization. The role of Nestin in the cellular architecture allows it to contribute to processes such as cell migration and proliferation critical during tissue development and repair.
Nestin integrates into networks that regulate stem cell behavior and neurogenesis. It tightly interacts within the pathways involving cell cycle regulation and differentiation. For example the Wnt and Notch signaling pathways modulate its expression to influence neural stem cell fate and patterning during development. Protein interactions between Nestin and other cytoskeletal elements like vimentin facilitate its roles in these pathways adapting the cellular environment for specific developmental activities.
Nestin associates with various pathological conditions including brain tumors and muscular dystrophies. Its expression is aberrant in many forms of gliomas where it often signifies a higher grade of malignancy. Within these tumors Nestin connects to oncogenic signaling pathways involving proteins such as the epidermal growth factor receptor (EGFR). Moreover in muscular dystrophies Nestin's expression in regenerating muscle fibers illustrates its involvement in cellular repair mechanisms potentially interacting with dystrophin a protein important for muscle integrity. Research on anti-Nestin antibodies continues to clarify its role in these diseases potentially offering diagnostic or therapeutic insights.
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Immunocytochemistry/Immunofluorescence analysis of 3T3 cells labelling Nestin (green) with ab134017. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-Beta-Tubulin I, Blue - Hoechst stain for nuclei.
Nestin Western blot staining of neonatal mouse brain using chicken Anti-Nestin antibody
All lanes: Western blot - Anti-Nestin antibody - Neural Stem Cell Marker (ab134017) at 1/10000 dilution
All lanes: neonatal mouse brain
Predicted band size: 177 kDa
Nestin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using chicken Anti-Nestin antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse hippocampus tissue labelling Nestin (red) with ab134017. Tissue was fixed with 10% formalin. Green - rabbit anti-Synaptotagmin, Blue - Hoechst stain for nuclei.
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