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Chicken Polyclonal Nestin antibody. Suitable for ICC/IF, WB, IHC-P and reacts with Mouse samples. Cited in 31 publications. Immunogen corresponding to Synthetic Peptide within Mouse Nes conjugated to Keyhole Limpet Haemocyanin.


Images

Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody (AB134017), expandable thumbnail
  • Western blot - Anti-Nestin antibody - Neural Stem Cell Marker (AB134017), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody - Neural Stem Cell Marker (AB134017), expandable thumbnail

Publications

Key facts

Isotype
IgY
Host species
Chicken
Storage buffer

pH: 7
Preservative: 0.02% Sodium azide
Constituents: 99% PBS

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Synthetic Peptide within Mouse Nes conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q6P5H2

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-P
Mouse
Tested
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/2000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/20000 - 1/50000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/2000
Notes

-

Associated Products

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Target data

Function

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division (By similarity). Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Alternative names

Recommended products

Chicken Polyclonal Nestin antibody. Suitable for ICC/IF, WB, IHC-P and reacts with Mouse samples. Cited in 31 publications. Immunogen corresponding to Synthetic Peptide within Mouse Nes conjugated to Keyhole Limpet Haemocyanin.

Key facts

Isotype
IgY
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Synthetic Peptide within Mouse Nes conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q6P5H2
Purification technique
Affinity purification Immunogen
Concentration
Loading...
Purification notes

IgY fractions were purified from the yolks and IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 300 µg/ml, and the preparation filter-sterilized.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle, Store undiluted

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Nestin also known as NES is an intermediate filament protein with a molecular weight of about 220 kDa. Nestin features prominently in cells related to the nervous system including neural stem cells and it serves as a structural component in these cells. Nestin also appears in substantial amounts during the development of the nervous system and is important for cell structural organization. It is often used as a Nestin marker in research particularly with Nestin antibodies for identifying the presence of neural stem cells and tracking their developmental course. Researchers frequently utilize techniques like Nestin IHC (immunohistochemistry) and Nestin western blot to stain and detect Nestin allowing detailed investigations into its expression in various tissues.

Biological function summary

Nestin plays a significant role in the dynamic remodeling of the cytoskeleton. It does not act as a solitary element but forms part of a larger protein complex involved in maintaining and organizing cellular structure. This property grants cells flexibility in shape and resilience during the rapid changes in development and specialization. The role of Nestin in the cellular architecture allows it to contribute to processes such as cell migration and proliferation critical during tissue development and repair.

Pathways

Nestin integrates into networks that regulate stem cell behavior and neurogenesis. It tightly interacts within the pathways involving cell cycle regulation and differentiation. For example the Wnt and Notch signaling pathways modulate its expression to influence neural stem cell fate and patterning during development. Protein interactions between Nestin and other cytoskeletal elements like vimentin facilitate its roles in these pathways adapting the cellular environment for specific developmental activities.

Associated diseases and disorders

Nestin associates with various pathological conditions including brain tumors and muscular dystrophies. Its expression is aberrant in many forms of gliomas where it often signifies a higher grade of malignancy. Within these tumors Nestin connects to oncogenic signaling pathways involving proteins such as the epidermal growth factor receptor (EGFR). Moreover in muscular dystrophies Nestin's expression in regenerating muscle fibers illustrates its involvement in cellular repair mechanisms potentially interacting with dystrophin a protein important for muscle integrity. Research on anti-Nestin antibodies continues to clarify its role in these diseases potentially offering diagnostic or therapeutic insights.

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3 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody (ab134017), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody (ab134017)

    Immunocytochemistry/Immunofluorescence analysis of 3T3 cells labelling Nestin (green) with ab134017. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-Beta-Tubulin I, Blue - Hoechst stain for nuclei.

  • Western blot - Anti-Nestin antibody - Neural Stem Cell Marker (ab134017), expandable thumbnail

    Western blot - Anti-Nestin antibody - Neural Stem Cell Marker (ab134017)

    Nestin Western blot staining of neonatal mouse brain using chicken Anti-Nestin antibody

    All lanes: Western blot - Anti-Nestin antibody - Neural Stem Cell Marker (ab134017) at 1/10000 dilution

    All lanes: neonatal mouse brain

    Predicted band size: 177 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody - Neural Stem Cell Marker (ab134017), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody - Neural Stem Cell Marker (ab134017)

    Nestin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using chicken Anti-Nestin antibody

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse hippocampus tissue labelling Nestin (red) with ab134017. Tissue was fixed with 10% formalin. Green - rabbit anti-Synaptotagmin, Blue - Hoechst stain for nuclei.

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