Anti-Nestin antibody - Neural Stem Cell Marker

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody - Neural Stem Cell Marker (AB134017)

Immunocytochemistry/Immunofluorescence analysis of 3T3 cells labelling Nestin (green) with ab134017. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-Beta-Tubulin I, Blue - Hoechst stain for nuclei.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody - Neural Stem Cell Marker (AB134017)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse hippocampus tissue labelling Nestin (red) with ab134017. Tissue was fixed with 10% formalin. Green - rabbit anti-Synaptotagmin, Blue - Hoechst stain for nuclei.

• WB

Unknown

Western blot - Anti-Nestin antibody - Neural Stem Cell Marker (AB134017)

All lanes:

Western blot - Anti-Nestin antibody - Neural Stem Cell Marker (ab134017) at 1/10000 dilution

All lanes:

neonatal mouse brain

Predicted band size: 177 kDa

false

• ICC/IF

CiteAb

Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody - Neural Stem Cell Marker (AB134017)

Immunocytochemistry-immunofluorescence using Anti-Nestin antibody, ab134017. Publication image from Peruzzotti-Jametti, L. et al., 2018, Cell Stem Cell, 29478844. Legend direct from paper.

NSCs Transplantation Ameliorates Chronic Neuroinflammation and Reduces Succinate Levels in the CSF of EAE Mice(A–D) Representative images of fGFP+ iNSCs at 30 dpt expressing the proliferation marker Ki67 (A, arrowheads) and the neural marker Nestin (A), the mature neuronal marker NeuN (B, arrowhead), the astroglial lineage marker GFAP (C), or the oligodendroglial lineage marker OLIG2 (D, arrowhead).(E) Confocal microscopy image of a perivascular area with several fGFP+ iNSCs in juxtaposition to fGFP−/F4/80+ MPs. Nuclei in (A)–(E) are stained with DAPI (blue).(F) Behavioral outcome of iNSCs/NSCs-transplanted EAE mice. Data are mean EAE score (±SEM) from n ≥ 7 mice/group over n = 2 independent experiments. EAE mice injected icv with mouse fibroblasts (MFs) or PBS were used as controls.(G and H) Flow-cytometry-based ex vivo quantification of the expression levels of type 1 inflammatory (CD80) and anti-inflammatory (MRC1) markers in CX3CR1+ microglial cells (G) and CCR2+ monocyte-derived infiltrating macrophages (H) from the CNS of iNSC- and NSC-transplanted EAE mice at 30 dpt. Quantitative data are shown on the left, whereas representative density plots are shown on the right. Data are min to max % of marker-positive cells from n ≥ 4 pools of mice/group.(I) Representative confocal microscopy image and comparative histograms of a perivascular area with several fGFP+ iNSCs in juxtaposition to F4/80+ MPs. Low iNOS and prevalent MRC1 expression is detected in F4/80+ MPs close to fGFP+ iNSCs (inset on the left), whereas high iNOS expression is observed in the remaining MP infiltrate (inset on the right). Nuclei are stained with DAPI.(J) Expression levels (qRT-PCR) of pro- and anti-inflammatory genes in the brain and spinal cord of EAE mice. Data are mean fold change over HC from n ≥ 3 mice/group.(K and L) Quantification and representative 3D reconstructions of spinal cord damage in iNSC- and NSC-transplanted EAE mice. Data are mean % of Bielschowsky negative-stained axonal loss (K) or Luxol fast blue (LFB) negative-stained demyelinated (L) areas/spinal cord section (±SEM) from n ≥ 5 mice/group over n = 2 independent experiments.(M) Levels of CSF metabolites significantly changed during EAE (versus HC). Corresponding levels in matched plasma samples are also shown. Data are mean a.u. (±SEM) from n ≥ 3 mice/group.The scale bars represent 25 µm (A–E), 50 µm (I), and 2 mm (K and L). ∗p ≤ 0.05 and ∗∗p ≤ 0.01 versus PBS; #p ≤ 0.05 versus HC; dpt, days post-transplantation; FI, fluorescence intensity; HC, healthy controls; PD, peak of disease. See also Figures S1, S2, and S3 and Table S1.