AB7659

Anti-Nestin antibody - Neural Stem Cell Marker

Name: Anti-Nestin antibody - Neural Stem Cell Marker (ab7659) | Abcam
Brand: Abcam Limited
SKU: AB7659
Price: 400 USD
Availability: InStock
Rating: 5 (9 reviews)

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What is this?

(9 Reviews)

(20 Publications)

Rabbit Polyclonal Nestin antibody. Suitable for WB and reacts with Mouse samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Human NES aa 1450-1500.

View Alternative Names

Nbla00170, NES, Nestin

2 Images

Collaborator

Western blot - Anti-Nestin antibody - Neural Stem Cell Marker (AB7659)

A slightly higher band obtained than expected, but as predicted in the datasheet. Denaturing of the WB lysate results in a loss of Nestin specific immunoreactivity. NB : Equal protein loading was verified.

All lanes:

Western blot - Anti-Nestin antibody - Neural Stem Cell Marker (ab7659) at 1 µg/mL

Lane 1:

Mouse neural precursors whole cell lysate reduced but not denatured at 10 µg

Lane 2:

Mouse neural precursors whole cell lysate reduced and denatured at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP at 1/20000 dilution

Predicted band size: 207 kDa

Observed band size: 230 kDa

true

Exposure time: 10min

This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, France

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Western blot - Anti-Nestin antibody - Neural Stem Cell Marker (AB7659)

All lanes:

Western blot - Anti-Nestin antibody - Neural Stem Cell Marker (ab7659) at 1/2000 dilution

All lanes:

MEF whole cell lysate at 30 µg

Secondary

All lanes:

IRDye 800conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution

Predicted band size: 207 kDa

Observed band size: 200 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Synthetic Peptide within Human NES aa 1450-1500. The exact immunogen used to generate this antibody is proprietary information.

P48681

Specificity

Detects a band at 200kDa representing Nestin in MEFs. Denaturing of WB protein lysate causes loss of Nestin immunoreactivity in WB.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "Do not denature WB protein lysate, denaturing results in loss of Nestin specific immunoreactivity (See Abreview).", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab7659 has been upgraded from Fast Track status based on data obtained in a new batch of this antibody.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This affinity purified antibody is directed against human Nestin protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nestin also known as NES is an intermediate filament protein with a molecular weight of about 220 kDa. Nestin features prominently in cells related to the nervous system including neural stem cells and it serves as a structural component in these cells. Nestin also appears in substantial amounts during the development of the nervous system and is important for cell structural organization. It is often used as a Nestin marker in research particularly with Nestin antibodies for identifying the presence of neural stem cells and tracking their developmental course. Researchers frequently utilize techniques like Nestin IHC (immunohistochemistry) and Nestin western blot to stain and detect Nestin allowing detailed investigations into its expression in various tissues.

Biological function summary

Nestin plays a significant role in the dynamic remodeling of the cytoskeleton. It does not act as a solitary element but forms part of a larger protein complex involved in maintaining and organizing cellular structure. This property grants cells flexibility in shape and resilience during the rapid changes in development and specialization. The role of Nestin in the cellular architecture allows it to contribute to processes such as cell migration and proliferation critical during tissue development and repair.

Pathways

Nestin integrates into networks that regulate stem cell behavior and neurogenesis. It tightly interacts within the pathways involving cell cycle regulation and differentiation. For example the Wnt and Notch signaling pathways modulate its expression to influence neural stem cell fate and patterning during development. Protein interactions between Nestin and other cytoskeletal elements like vimentin facilitate its roles in these pathways adapting the cellular environment for specific developmental activities.

Nestin associates with various pathological conditions including brain tumors and muscular dystrophies. Its expression is aberrant in many forms of gliomas where it often signifies a higher grade of malignancy. Within these tumors Nestin connects to oncogenic signaling pathways involving proteins such as the epidermal growth factor receptor (EGFR). Moreover in muscular dystrophies Nestin's expression in regenerating muscle fibers illustrates its involvement in cellular repair mechanisms potentially interacting with dystrophin a protein important for muscle integrity. Research on anti-Nestin antibodies continues to clarify its role in these diseases potentially offering diagnostic or therapeutic insights.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).

See full target information NES

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology reports 51:1076 PubMed39425753

2024

Progesterone increases metabolism via the pentose phosphate pathway in bovine uterine epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Malia D Berg,Camila U Braz,Matthew Dean

JCI insight 9: PubMed39172519

2024

Hypoxia-induced complement component 3 promotes aggressive tumor growth in the glioblastoma microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca Rosberg,Karolina I Smolag,Jonas Sjölund,Elinn Johansson,Christina Bergelin,Julia Wahldén,Vasiliki Pantazopoulou,Crister Ceberg,Kristian Pietras,Anna M Blom,Alexander Pietras

International journal of molecular sciences 23: PubMed36142200

2022

microRNAs Control Antiviral Immune Response, Cell Death and Chemotaxis Pathways in Human Neuronal Precursor Cells (NPCs) during Zika Virus Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina M Polonio,Patrick da Silva,Fabiele B Russo,Brendo R N Hyppolito,Nagela G Zanluqui,Cecília Benazzato,Patrícia C B Beltrão-Braga,Sandra M Muxel,Jean Pierre S Peron

Animals : an open access journal from MDPI 12: PubMed35625130

2022

Isolation and Characterization of Cat Olfactory Ecto-Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Marie-Laure Mollichella,Violaine Mechin,Dany Royer,Patrick Pageat,Pietro Asproni

Journal of cellular biochemistry 123:547-567 PubMed34958137

2021

Suppression of transient receptor potential melastatin 7 regulates pluripotency, proliferation, and differentiation of mouse embryonic stem cells via mechanistic target of rapamycin-extracellular signal-regulated kinase activation.

Applications

Unspecified application

Species

Unspecified reactive species

Wansoo Kim,Song Park,Wookbong Kwon,Daehwan Kim,Jin-Kyu Park,Jee Eun Han,Gil-Jae Cho,Se-Hyeon Han,Yonghun Sung,Jun-Koo Yi,Myoung Ok Kim,Zae Young Ryoo,Seong-Kyoon Choi

eLife 10: PubMed33554859

2021

TWIST1 and chromatin regulatory proteins interact to guide neural crest cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaochen Fan,V Pragathi Masamsetti,Jane Qj Sun,Kasper Engholm-Keller,Pierre Osteil,Joshua Studdert,Mark E Graham,Nicolas Fossat,Patrick Pl Tam

Molecular metabolism :101164 PubMed33453419

2021

Essential roles of insulin and IGF-1 receptors during embryonic lineage development.

Applications

Unspecified application

Species

Unspecified reactive species

Erin R Okawa,Manoj K Gupta,Sevim Kahraman,Praneeth Goli,Masaji Sakaguchi,Jiang Hu,Kaiti Duan,Brittany Slipp,Jochen K Lennerz,Rohit N Kulkarni

Translational psychiatry 10:331 PubMed32989216

2020

N,N-dimethyltryptamine compound found in the hallucinogenic tea ayahuasca, regulates adult neurogenesis in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Jose A Morales-Garcia,Javier Calleja-Conde,Jose A Lopez-Moreno,Sandra Alonso-Gil,Marina Sanz-SanCristobal,Jordi Riba,Ana Perez-Castillo

Nature communications 11:2984 PubMed32533114

2020

ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyun Sun,Xixia Peng,Yuqin Cao,Yan Zhou,Yuhua Sun

Journal of cellular biochemistry 121:4667-4679 PubMed32065444

2020

Inhibition of MAGEA2 regulates pluripotency, proliferation, apoptosis, and differentiation in mouse embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Song Park,Jee Eun Han,Hyeon-Gyeom Kim,Hee-Yeon Kim,Min-Gi Kim,Jin-Kyu Park,Gil-Jae Cho,Hai Huang,Myoung Ok Kim,Zae Young Ryoo,Se-Hyeon Han,Seong-Kyoon Choi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Nestin antibody - Neural Stem Cell Marker

Western blot - Anti-Nestin antibody - Neural Stem Cell Marker (AB7659)

Western blot - Anti-Nestin antibody - Neural Stem Cell Marker (AB7659)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (20)

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