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AB92391

Anti-Nestin antibody - Neural Stem Cell Marker

4

(7 Reviews)

|

(60 Publications)

Rabbit Polyclonal Nestin antibody. Suitable for ICC/IF and reacts with Rat samples. Cited in 60 publications.

View Alternative Names

Nbla00170, NES, Nestin

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody - Neural Stem Cell Marker (AB92391)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody - Neural Stem Cell Marker (AB92391)

Immunocytochemical localisation of Nestin using ab92391 on E16 rat hippocampal cell culture. The image shows nestin positive rat hippocampal cells isolated from embryonic day 16 (grown in serum free conditions for 2-3 weeks). Cover slips were fixed in 5% PFA in 0.1M PBS (1xPBS) for 5 minutes and washed with 0.1M PBS. Permeabilisation : 0.3% Triton X-100. Primary antibody ab92391 was diluted 1/250 and incubated for 2h @ RT. Secondary antibody : donkey anti-rabbit Alexa Fluor 488 (1/2000). DAPI in blue stains nuclei.

This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody - Neural Stem Cell Marker (AB92391)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody - Neural Stem Cell Marker (AB92391)

ICC/IF image of ab92391 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92391, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody - Neural Stem Cell Marker (AB92391)
  • ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody - Neural Stem Cell Marker (AB92391)

Immunocytochemical localisation of Nestin using ab92391 on P0 rat retinal culture. The image shows a nestin positive retinal ganglionic cell from P0 retina that has been grown for 7 days on a glass coverslip. Cover slips were fixed in 5% PFA in 0.1M PBS (1xPBS) for 5 minutes and washed with 0.1M PBS. Permeabilisation : 0.3% Triton X-100. Primary antibody ab92391 was diluted 1/250 and incubated for 2h @ RT. Secondary antibody : donkey anti-rabbit Alexa Fluor 488 (1/2000).

This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunoreactivity detected with Rabbit polyclonal to Nestin (ab92391) does not overlap in with that of the mature neuronal markers such as MAP2, NeuN, Neurofilament L and Beta-3-tublin (data available on request).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFoFr-species-checked": "notRecommended", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCFoFr-species-checked": "notRecommended", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab92391 is raised using the same immunogen as ab5968. Batches sold under ab92391 have passed QC testing in ICC/IF only. In our hands, this product did not work in IHC-P. For further information please contact our Customer Services team.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nestin also known as NES is an intermediate filament protein with a molecular weight of about 220 kDa. Nestin features prominently in cells related to the nervous system including neural stem cells and it serves as a structural component in these cells. Nestin also appears in substantial amounts during the development of the nervous system and is important for cell structural organization. It is often used as a Nestin marker in research particularly with Nestin antibodies for identifying the presence of neural stem cells and tracking their developmental course. Researchers frequently utilize techniques like Nestin IHC (immunohistochemistry) and Nestin western blot to stain and detect Nestin allowing detailed investigations into its expression in various tissues.
Biological function summary

Nestin plays a significant role in the dynamic remodeling of the cytoskeleton. It does not act as a solitary element but forms part of a larger protein complex involved in maintaining and organizing cellular structure. This property grants cells flexibility in shape and resilience during the rapid changes in development and specialization. The role of Nestin in the cellular architecture allows it to contribute to processes such as cell migration and proliferation critical during tissue development and repair.

Pathways

Nestin integrates into networks that regulate stem cell behavior and neurogenesis. It tightly interacts within the pathways involving cell cycle regulation and differentiation. For example the Wnt and Notch signaling pathways modulate its expression to influence neural stem cell fate and patterning during development. Protein interactions between Nestin and other cytoskeletal elements like vimentin facilitate its roles in these pathways adapting the cellular environment for specific developmental activities.

Nestin associates with various pathological conditions including brain tumors and muscular dystrophies. Its expression is aberrant in many forms of gliomas where it often signifies a higher grade of malignancy. Within these tumors Nestin connects to oncogenic signaling pathways involving proteins such as the epidermal growth factor receptor (EGFR). Moreover in muscular dystrophies Nestin's expression in regenerating muscle fibers illustrates its involvement in cellular repair mechanisms potentially interacting with dystrophin a protein important for muscle integrity. Research on anti-Nestin antibodies continues to clarify its role in these diseases potentially offering diagnostic or therapeutic insights.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).
See full target information NES
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Publications (60)

Recent publications for all applications. Explore the full list and refine your search

Materials today. Bio 34:102172 PubMed40822932

2025

Neuroactive network tissue based on dual-factor neuroregenerative bioactive coating scaffolds and neural stem cells for spinal cord injury repair.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyi Liu,Wenhao Zhu,Zheng Wan,Cong Fu,Xiaoyu Zhang,Wenzhong Li,Wenchen Li,Zhenxu Wu,Min Guo,Mengtuan Long,Feiyang Yang,Hongyu Chen,Xingcheng Yi,Honglei Wang,Peibiao Zhang,Haifeng Wang

Neuron 113:701-718.e8 PubMed39814010

2025

CRISPRi-based screens in iAssembloids to elucidate neuron-glia interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Emmy Li,Camila Benitez,Steven C Boggess,Mark Koontz,Indigo V L Rose,Delsy Martinez,Nina Dräger,Olivia M Teter,Avi J Samelson,Na'im Pierce,Erik M Ullian,Martin Kampmann

iScience 27:110613 PubMed39224516

2024

CelltypeR: A flow cytometry pipeline to characterize single cells from brain organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Rhalena A Thomas,Julien Sirois,Shuming Li,Alexandre Gestin,Ghislaine Deyab,Valerio E C Piscopo,Paula Lépine,Meghna Mathur,Carol X-Q Chen,Vincent Soubannier,Taylor M Goldsmith,Lama Fawaz,Thomas M Durcan,Edward A Fon

Heliyon 10:e30730 PubMed38784548

2024

Impact of subthalamic nucleus deep brain stimulation at different frequencies on neurogenesis in a rat model of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Wu,Zhiwei Ren,Runshi Gao,Ke Sun,Fangling Sun,Tingting Liu,Songyang Zheng,Wen Wang,Guojun Zhang

iScience 27:109166 PubMed38433895

2024

Homozygous ALS-linked mutations in TARDBP/TDP-43 lead to hypoactivity and synaptic abnormalities in human iPSC-derived motor neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Lépine,Angela Nauleau-Javaudin,Eric Deneault,Carol X-Q Chen,Narges Abdian,Anna Krystina Franco-Flores,Ghazal Haghi,María José Castellanos-Montiel,Gilles Maussion,Mathilde Chaineau,Thomas Martin Durcan

Scientific reports 14:3329 PubMed38337011

2024

Cellular senescence is associated with osteonecrosis of the femoral head while mesenchymal stem cell conditioned medium inhibits bone collapse.

Applications

Unspecified application

Species

Unspecified reactive species

Masanori Okamoto,Hiroaki Nakashima,Kiyoshi Sakai,Yasuhiko Takegami,Yusuke Osawa,Junna Watanabe,Sadayuki Ito,Hideharu Hibi,Shiro Imagama

iScience 26:108497 PubMed38213789

2024

FEZ1 participates in human embryonic brain development by modulating neuronal progenitor subpopulation specification and migrations.

Applications

Unspecified application

Species

Unspecified reactive species

Yinghua Qu,Jonathan Jun-Yong Lim,Omer An,Henry Yang,Yi-Chin Toh,John Jia En Chua

Neurobiology of disease 190:106367 PubMed38042508

2023

Proteomic analysis of X-linked dystonia parkinsonism disease striatal neurons reveals altered RNA metabolism and splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Kizito-Tshitoko Tshilenge,Joanna Bons,Carlos Galicia Aguirre,Cristian Geronimo-Olvera,Samah Shah,Jacob Rose,Akos A Gerencser,Sally K Mak,Michelle E Ehrlich,D Cristopher Bragg,Birgit Schilling,Lisa M Ellerby

Molecular & cellular proteomics : MCP 22:100534 PubMed36958627

2023

Proteomic Analysis of Huntington's Disease Medium Spiny Neurons Identifies Alterations in Lipid Droplets.

Applications

Unspecified application

Species

Unspecified reactive species

Kizito-Tshitoko Tshilenge,Carlos Galicia Aguirre,Joanna Bons,Akos A Gerencser,Nathan Basisty,Sicheng Song,Jacob Rose,Alejandro Lopez-Ramirez,Swati Naphade,Ashley Loureiro,Elena Battistoni,Mateus Milani,Cameron Wehrfritz,Anja Holtz,Claudio Hetz,Sean D Mooney,Birgit Schilling,Lisa M Ellerby

Neuropathology : official journal of the Japanese Society of Neuropathology 43:362-372 PubMed36918198

2023

Intracerebral hirudin injection alleviates cognitive impairment and oxidative stress and promotes hippocampal neurogenesis in rats subjected to cerebral ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Xianfeng Xia,Min Li,Renxian Wei,Jin Li,Yulin Lei,Meikui Zhang
View all publications
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