Rabbit Polyclonal Nestin antibody. Suitable for ICC/IF and reacts with Rat samples. Cited in 41 publications.
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- Molecular & cellular proteomics : MCP 22:1005342023Proteomic Analysis of Huntington's Disease Medium Spiny Neurons Identifies Alterations in Lipid Droplets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKizito-Tshitoko Tshilenge,Carlos Galicia Aguirre,Joanna Bons,Akos A Gerencser,Nathan Basisty,Sicheng Song,Jacob Rose,Alejandro Lopez-Ramirez,Swati Naphade,Ashley Loureiro,Elena Battistoni,Mateus Milani,Cameron Wehrfritz,Anja Holtz,Claudio Hetz,Sean D Mooney,Birgit Schilling,Lisa M EllerbyPubMed 36958627
- Neuropathology : official journal of the Japanese Society of Neuropathology 43:362-3722023Intracerebral hirudin injection alleviates cognitive impairment and oxidative stress and promotes hippocampal neurogenesis in rats subjected to cerebral ischemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXianfeng Xia,Min Li,Renxian Wei,Jin Li,Yulin Lei,Meikui ZhangPubMed 36918198
- Journal of the Association for Research in Otolaryngology : JARO 23:467-4892022MicroRNA Signature and Cellular Characterization of Undifferentiated and Differentiated House Ear Institute-Organ of Corti 1 (HEI-OC1) Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPrintha Wijesinghe,Desmond A Nunez,Cathie GarnisPubMed 35546217
- Journal of tissue engineering and regenerative medicine 15:948-9632021Decellularized spinal cord meninges extracellular matrix hydrogel that supports neurogenic differentiation and vascular structure formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEren Ozudogru,Melis Isik,Cemil Can Eylem,Emirhan Nemutlu,Yavuz Emre Arslan,Burak DerkusPubMed 34463042
- Cells 10:2021Photobiomodulation with a 660-Nanometer Light-Emitting Diode Promotes Cell Proliferation in Astrocyte Culture.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSung-Ryeong Yoon,Namgue Hong,Min-Young Lee,Jin-Chul AhnPubMed 34359834
- Frontiers in neuroanatomy 15:6724122021Self-Regulation of Cerebral Metabolism and Its Neuroprotective Effect After Hypoxic-Ischemic Injury: Evidence From H-MRS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKexin Li,Yang Zheng,Xiaoming WangPubMed 34220456
- Autophagy 17:4119-41402021Modulating FKBP5/FKBP51 and autophagy lowers HTT (huntingtin) levels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBarbara J Bailus,Stephen M Scheeler,Jesse Simons,Maria A Sanchez,Kizito-Tshitoko Tshilenge,Jordi Creus-Muncunill,Swati Naphade,Alejandro Lopez-Ramirez,Ningzhe Zhang,Kuruwitage Lakshika Madushani,Stanislav Moroz,Ashley Loureiro,Katherine H Schreiber,Felix Hausch,Brian K Kennedy,Michelle E Ehrlich,Lisa M EllerbyPubMed 34024231
- Cells 10:2021Functional Expression of Choline Transporters in Human Neural Stem Cells and Its Link to Cell Proliferation, Cell Viability, and Neurite Outgrowth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYosuke Fujita,Tomoki Nagakura,Hiroyuki Uchino,Masato Inazu,Tsuyoshi YamanakaPubMed 33672580
- Frontiers in molecular neuroscience 13:6171432021Chronic Intermittent Hypobaric Hypoxia Restores Hippocampus Function and Rescues Cognitive Impairments in Chronic Epileptic Rats Wnt/β-catenin Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCan Sun,Jian Fu,Zhenzhen Qu,Lijing Jia,Dongxiao Li,Junli Zhen,Weiping WangPubMed 33584201
- Molecular medicine reports 23:2021Prenatal ethanol exposure impairs the formation of radial glial fibers and promotes the transformation of GFAPδ‑positive radial glial cells into astrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Li,Li-Na Zhang,Li Chong,Yue Liu,Feng-Yu Xi,Hong Zhang,Xiang-Long DuanPubMed 33576465
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-FoFr | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Not recommended | Not recommended | Predicted |
| Rat | Not recommended | Not recommended | Tested |
IHC-FoFr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/250 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).
Alternative names
Nbla00170, NES, Nestin
Recommended products
Rabbit Polyclonal Nestin antibody. Suitable for ICC/IF and reacts with Rat samples. Cited in 41 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
The immunoreactivity detected with Rabbit polyclonal to Nestin (ab92391) does not overlap in with that of the mature neuronal markers such as MAP2, NeuN, Neurofilament L and Beta-3-tublin (data available on request).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab92391 is raised using the same immunogen as ab5968. Batches sold under ab92391 have passed QC testing in ICC/IF only. In our hands, this product did not work in IHC-P. For further information please contact our Customer Services team.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nestin also known as NES is an intermediate filament protein with a molecular weight of about 220 kDa. Nestin features prominently in cells related to the nervous system including neural stem cells and it serves as a structural component in these cells. Nestin also appears in substantial amounts during the development of the nervous system and is important for cell structural organization. It is often used as a Nestin marker in research particularly with Nestin antibodies for identifying the presence of neural stem cells and tracking their developmental course. Researchers frequently utilize techniques like Nestin IHC (immunohistochemistry) and Nestin western blot to stain and detect Nestin allowing detailed investigations into its expression in various tissues.
- Biological function summary
Nestin plays a significant role in the dynamic remodeling of the cytoskeleton. It does not act as a solitary element but forms part of a larger protein complex involved in maintaining and organizing cellular structure. This property grants cells flexibility in shape and resilience during the rapid changes in development and specialization. The role of Nestin in the cellular architecture allows it to contribute to processes such as cell migration and proliferation critical during tissue development and repair.
- Pathways
Nestin integrates into networks that regulate stem cell behavior and neurogenesis. It tightly interacts within the pathways involving cell cycle regulation and differentiation. For example the Wnt and Notch signaling pathways modulate its expression to influence neural stem cell fate and patterning during development. Protein interactions between Nestin and other cytoskeletal elements like vimentin facilitate its roles in these pathways adapting the cellular environment for specific developmental activities.
- Associated diseases and disorders
Nestin associates with various pathological conditions including brain tumors and muscular dystrophies. Its expression is aberrant in many forms of gliomas where it often signifies a higher grade of malignancy. Within these tumors Nestin connects to oncogenic signaling pathways involving proteins such as the epidermal growth factor receptor (EGFR). Moreover in muscular dystrophies Nestin's expression in regenerating muscle fibers illustrates its involvement in cellular repair mechanisms potentially interacting with dystrophin a protein important for muscle integrity. Research on anti-Nestin antibodies continues to clarify its role in these diseases potentially offering diagnostic or therapeutic insights.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United KingdomImmunocytochemistry/ Immunofluorescence - Anti-Nestin antibody - Neural Stem Cell Marker (ab92391)
Immunocytochemical localisation of Nestin using ab92391 on P0 rat retinal culture. The image shows a nestin positive retinal ganglionic cell from P0 retina that has been grown for 7 days on a glass coverslip. Cover slips were fixed in 5% PFA in 0.1M PBS (1xPBS) for 5 minutes and washed with 0.1M PBS. Permeabilisation: 0.3% Triton X-100. Primary antibody ab92391 was diluted 1/250 and incubated for 2h @ RT. Secondary antibody: donkey anti-rabbit Alexa Fluor 488 (1/2000).
This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United KingdomImmunocytochemistry/ Immunofluorescence - Anti-Nestin antibody - Neural Stem Cell Marker (ab92391)
Immunocytochemical localisation of Nestin using ab92391 on E16 rat hippocampal cell culture. The image shows nestin positive rat hippocampal cells isolated from embryonic day 16 (grown in serum free conditions for 2-3 weeks). Cover slips were fixed in 5% PFA in 0.1M PBS (1xPBS) for 5 minutes and washed with 0.1M PBS. Permeabilisation: 0.3% Triton X-100. Primary antibody ab92391 was diluted 1/250 and incubated for 2h @ RT. Secondary antibody: donkey anti-rabbit Alexa Fluor 488 (1/2000). DAPI in blue stains nuclei.
Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody - Neural Stem Cell Marker (ab92391)
ICC/IF image of ab92391 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92391, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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