AB6142

Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker

Name: Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (ab6142) | Abcam
Brand: Abcam Limited
SKU: AB6142
Price: 165 USD
Availability: InStock
Rating: 4 (28 reviews)

BOND RX™ Validated
Lab Essentials
What is this?

(28 Reviews)

(280 Publications)

Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (ab6142) is a mouse monoclonal antibody detecting Nestin in Western Blot, IHC-P. Suitable for Mouse, Rat.

- Over 220 publications
- Trusted since 2003

View Alternative Names

Nestin, Nes

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (AB6142)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (AB6142)

IHC image of Nestin staining in a section of formalin-fixed paraffin-embedded normal rat brain performed on a Leica BOND^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab6142, 0.05ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC-P

AbReview12138****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (AB6142)

ab6142 staining adult mouse brain tissue section by Immunohistochemistry (Formalin/PFA-fixed, paraffin embedded sections). Tissue underwent fixation in paraformaldehyde, heat mediated antigen retrieval in Sodium Citrate, permeabilization in 1% Triton buffer and blocking in 10% serum for 1 hour at 25°C. The primary antibody, diluted 1/200 (PBS, 2% Donkey serum, 0.2% Triton) for 16 hours at 4°C. An Alexa Fluor^® 488 conjugated donkey polyclonal to mouse Ig, diluted 1/500 was used as the secondary.

This image is courtesy of an anonymous abreview.

IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (AB6142)

REST expression in N-hREST mouse brains correlates with stemness in embryonic neural stem cells. Immunofluorescence analysis of E18.5 N-hREST and LSL-hRESTcontrol littermate mouse brains with antibodies against REST (using an antibody that preferentially recognizes hREST over mouse REST) and Nestin (using ab6142)

Mice were anesthetized and perfused with phosphate-buffered saline followed by 4% paraformaldehyde (PFA). Brain tissues were then dissected and fixed in 4% PFA overnight at 4 °C. Fixed brain tissues were processed for paraffin embedding and then cut into 5-μm sections.

Image from Lu L. et al., Sci Rep. 2018 Aug 14;8(1):12083. Fig2b. doi: 10.1038/s41598-018-29441-3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

Lab

Western blot - Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (AB6142)

This blot was produced using a 3-8% Tris-Acetate gel under the Tris-Acetate buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab6142 overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (ab6142) at 1 µg/mL

Lane 1:

Mouse E12 brain tissue lysate at 20 µg

Lane 2:

Mouse E14 brain tissue lysate at 20 µg

Lane 3:

Mouse E16 brain tissue lysate at 20 µg

Lane 4:

Mouse E18 brain tissue lysate at 20 µg

Lane 5:

Rat E12 brain tissue lysate at 20 µg

Lane 6:

Rat E14 brain tissue lysate at 20 µg

Lane 7:

Rat E16 brain tissue lysate at 20 µg

Lane 8:

Rat E18 brain tissue lysate at 20 µg

Predicted band size: 177 kDa

false

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Nestin antibody [Rat-401]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker
Carrier free

Anti-Nestin antibody [Rat-401] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Rat-401

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.05-1 µg/mL", "IHCP-species-notes": "Tissue fixed with 4% paraformaldehyde at pH 7.4 for light microscopy. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "Block with milk or BSA but do not dilute primary antibody in buffer containing milk.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.05-1 µg/mL", "IHCP-species-notes": "Tissue fixed with 4% paraformaldehyde at pH 7.4 for light microscopy. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "Block with milk or BSA but do not dilute primary antibody in buffer containing milk.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "Tissue fixed with 4% paraformaldehyde at pH 7.4 for light microscopy. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "Block with milk or BSA but do not dilute primary antibody in buffer containing milk.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Monkey": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "Tissue fixed with 4% paraformaldehyde at pH 7.4 for light microscopy. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "Block with milk or BSA but do not dilute primary antibody in buffer containing milk.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Sheep": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "Tissue fixed with 4% paraformaldehyde at pH 7.4 for light microscopy. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "Block with milk or BSA but do not dilute primary antibody in buffer containing milk.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (ab6142) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Mouse, Rat samples.

What is the molecular weight of Nestin?
Anti-Nestin [Rat-401] - Neural Stem Cell Marker (ab6142) specifically detects a band for Nestin (UniProt: P48681) at a molecular weight of 200kDa.

Trusted by the scientific community
Anti-Nestin [Rat-401] - Neural Stem Cell Marker (ab6142) was first used in a scientific publication in 2003 and has been cited over 220 times in peer-reviewed journals.

Reviewed by scientists
Anti-Nestin [Rat-401] - Neural Stem Cell Marker (ab6142) has over 25 independent reviews from customers.

Other related products
We have a range of other formats of antibody clone [Rat-401] also available for your convenience: ab6142, Alexa Fluor® 647 - ab196693, Alexa Fluor® 488 - ab197495, Carrier free - ab264111

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nestin also known as NES is an intermediate filament protein with a molecular weight of about 220 kDa. Nestin features prominently in cells related to the nervous system including neural stem cells and it serves as a structural component in these cells. Nestin also appears in substantial amounts during the development of the nervous system and is important for cell structural organization. It is often used as a Nestin marker in research particularly with Nestin antibodies for identifying the presence of neural stem cells and tracking their developmental course. Researchers frequently utilize techniques like Nestin IHC (immunohistochemistry) and Nestin western blot to stain and detect Nestin allowing detailed investigations into its expression in various tissues.

Biological function summary

Nestin plays a significant role in the dynamic remodeling of the cytoskeleton. It does not act as a solitary element but forms part of a larger protein complex involved in maintaining and organizing cellular structure. This property grants cells flexibility in shape and resilience during the rapid changes in development and specialization. The role of Nestin in the cellular architecture allows it to contribute to processes such as cell migration and proliferation critical during tissue development and repair.

Pathways

Nestin integrates into networks that regulate stem cell behavior and neurogenesis. It tightly interacts within the pathways involving cell cycle regulation and differentiation. For example the Wnt and Notch signaling pathways modulate its expression to influence neural stem cell fate and patterning during development. Protein interactions between Nestin and other cytoskeletal elements like vimentin facilitate its roles in these pathways adapting the cellular environment for specific developmental activities.

Nestin associates with various pathological conditions including brain tumors and muscular dystrophies. Its expression is aberrant in many forms of gliomas where it often signifies a higher grade of malignancy. Within these tumors Nestin connects to oncogenic signaling pathways involving proteins such as the epidermal growth factor receptor (EGFR). Moreover in muscular dystrophies Nestin's expression in regenerating muscle fibers illustrates its involvement in cellular repair mechanisms potentially interacting with dystrophin a protein important for muscle integrity. Research on anti-Nestin antibodies continues to clarify its role in these diseases potentially offering diagnostic or therapeutic insights.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division (By similarity). Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

See full target information Nes

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Publications (280)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neurology 16:1606606 PubMed40895108

2025

Intraventricular continuous BDNF administration ameliorates neuroinflammation and enhances neurogenesis against rodent intracerebral hemorrhage model.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Chun Lin,Masahito Kawabori,Erika Yoshie,Yo Nakahara,Li-Kai Tsai,Miki Fujimura

Scientific reports 15:29291 PubMed40789910

2025

Extremely low-frequency electromagnetic fields facilitate proliferation and functional differentiation in spinal neural stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wenxu Tang,Dan He,Xiaofei Li,Yi Feng,Yue Xu,Jiawei Hu,Wei Xu,Lei Xue

Stem cells international 2025:8828183 PubMed40746442

2025

ipRGCs Sensitive Blue Light Exposure Promotes the Robustness of Circadian and Neural Stem Cells in Sleep Deprived Conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoting Bu,Xiaotong Li,Jinyu Shi,Qiaozhen Qin,Heyang Zhang,Yuanrong Qiu,Lingyu Zhang,Yifei Tan,Hanping Shi,Xiaoxia Jiang

Frontiers in molecular neuroscience 18:1628860 PubMed40747242

2025

Tet3-mediated DNA demethylation is essential for maintaining the dedifferentiation capacity of mammalian Müller glia.

Applications

Unspecified application

Species

Unspecified reactive species

Erick J Martinez-Colin,Ivonne Lezama,Lenin D Ochoa-de la Paz,Monica Lamas

ACS biomaterials science & engineering 11:4941-4953 PubMed40637703

2025

Pre-Loading of Cells via Vapor Sublimation and the Deposition Polymerization Process with a 3D Porous Scaffold for Cell Cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Chung-Ju Chen,Chin-Yun Lee,Mei-Yu Chen,Ying-Hsuan Shi,Yu-Chih Chiang,Chen-Chi Wu,Hsien-Yeh Chen

Cell death & disease 16:479 PubMed40593476

2025

p66Shc deletion confers apoptotic resistance to loss of EGFR-ERK signalling in neural stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew M Powell,Robert C Cumming,Dean H Betts

Scientific reports 15:18737 PubMed40436940

2025

Lab life, seasons and chromosome fusions affect non-cell-autonomously proliferation and neurogenesis, but not oligodendrogenesis, in mice and voles.

Applications

Unspecified application

Species

Unspecified reactive species

Athanasia Rapti,Theodosia Androutsopoulou,Evangelia Andreopoulou,Maria Mellou,Georgios Leventakos,Maria Anesti,Konstantina Mastori,Myrto Chatzopoulou,Paraskevi Smyrli,Nikiforos Lakos,Kawthar Muse,Georgios P Mitsainas,Ilias Kazanis

Frontiers in pharmacology 16:1554945 PubMed40276612

2025

Tauroursodeoxycholic acid regulates macrophage/monocyte distribution and improves spinal microenvironment to promote nerve regeneration through inhibiting NF-κB signaling pathway in spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yonghui Hou,Yage Zhang,Lin Ma,Dan Luo,Wanshun Wang,Shunmei E,Chengxi Huang,Yu Hou,Shudong Chen,Jiheng Zhan,Liangliang Xu,Dingkun Lin

Theranostics 15:3837-3861 PubMed40213674

2025

Neural-enhancing PRP/Alg/GelMA triple-network hydrogel for neurogenesis and angiogenesis after spinal cord injury via PI3K/AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihe Yun,Jiuping Wu,Xinzhi Sun,Tao Yu,Wu Xue,Anyuan Dai,Tianyang Yuan,Xuhao Yang,Inbo Han,Yanting Liu,Wenlong Song,Qinyi Liu

PLoS biology 23:e3003100 PubMed40179071

2025

Gas1-high quiescent neural stem cells are multipotent and produce oligodendrocytes during aging and after demyelinating injury.

Applications

Unspecified application

Species

Unspecified reactive species

Chaoqiong Ding,Zhenzhong Pan,Xiang Yan,Ran Zhou,Huifang Li,Lu Chen,Yuan Wang,Yan Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (AB6142)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (AB6142)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (AB6142)

Western blot - Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (AB6142)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (280)

Product promise