Anti-Nestin antibody [SP103] - BSA and Azide free (ab271870)

Rabbit Recombinant Monoclonal Nestin antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IHC-Fr and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [SP103] - BSA and Azide free (AB271870), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	ICC/IF	Flow Cyt (Intra)	IHC-Fr
Human	Tested	Tested	Tested	Tested	Tested
Dog	Predicted	Predicted	Predicted	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Dog	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Antigen retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

Predicted
Predicted

Species	Dilution info	Notes
Species Dog	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Dog	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species	Dilution info	Notes
Species Dog	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Dog	Dilution info -	Notes -

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Target data

See full target information NES

Function

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).

Alternative names

Nbla00170, NES, Nestin

Recommended products

Rabbit Recombinant Monoclonal Nestin antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IHC-Fr and reacts with Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: SP103
Purification technique: Affinity purification Protein A/G
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab271870 is the carrier-free version of Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389.

Patented technology

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Nestin also known as NES is an intermediate filament protein with a molecular weight of about 220 kDa. Nestin features prominently in cells related to the nervous system including neural stem cells and it serves as a structural component in these cells. Nestin also appears in substantial amounts during the development of the nervous system and is important for cell structural organization. It is often used as a Nestin marker in research particularly with Nestin antibodies for identifying the presence of neural stem cells and tracking their developmental course. Researchers frequently utilize techniques like Nestin IHC (immunohistochemistry) and Nestin western blot to stain and detect Nestin allowing detailed investigations into its expression in various tissues.

Biological function summary

Nestin plays a significant role in the dynamic remodeling of the cytoskeleton. It does not act as a solitary element but forms part of a larger protein complex involved in maintaining and organizing cellular structure. This property grants cells flexibility in shape and resilience during the rapid changes in development and specialization. The role of Nestin in the cellular architecture allows it to contribute to processes such as cell migration and proliferation critical during tissue development and repair.

Pathways

Nestin integrates into networks that regulate stem cell behavior and neurogenesis. It tightly interacts within the pathways involving cell cycle regulation and differentiation. For example the Wnt and Notch signaling pathways modulate its expression to influence neural stem cell fate and patterning during development. Protein interactions between Nestin and other cytoskeletal elements like vimentin facilitate its roles in these pathways adapting the cellular environment for specific developmental activities.

Associated diseases and disorders

Nestin associates with various pathological conditions including brain tumors and muscular dystrophies. Its expression is aberrant in many forms of gliomas where it often signifies a higher grade of malignancy. Within these tumors Nestin connects to oncogenic signaling pathways involving proteins such as the epidermal growth factor receptor (EGFR). Moreover in muscular dystrophies Nestin's expression in regenerating muscle fibers illustrates its involvement in cellular repair mechanisms potentially interacting with dystrophin a protein important for muscle integrity. Research on anti-Nestin antibodies continues to clarify its role in these diseases potentially offering diagnostic or therapeutic insights.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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13 product images

Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [SP103] - BSA and Azide free (ab271870)
ICC/IF image of Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - BSA and Azide free (ab271870)
Formalin-fixed, paraffin-embedded human endometrium tissue stained for Nestin using Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389 at 1/100 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389).
This image is courtesy of Hongwei Shao by customer review.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - BSA and Azide free (ab271870)
Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389 staining Nestin in human melanoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using Tris EDTA pH 9.0. Samples were then permeabilized using 0.25% Triton X-100, blocked, then incubated with Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389 at a 1/100 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 594 (red) conjugated donkey anti-rabbit polyclonal used at a 1/100 dilution. (Green) tumor cells, (blue) DAPI.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389).
Flow Cytometry (Intracellular) - Anti-Nestin antibody [SP103] - BSA and Azide free (ab271870)
Overlay histogram showing SH-SY5Y cells stained with Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - BSA and Azide free (ab271870)
Formalin-fixed, paraffin-embedded human colon tissue stained for Nestin using Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389 at 1/100 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - BSA and Azide free (ab271870)
Formalin-fixed, paraffin-embedded human prostate tissue stained for Nestin using Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389 at 1/100 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - BSA and Azide free (ab271870)
Formalin-fixed, paraffin-embedded human lung tissue stained for Nestin using Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389 at 1/100 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - BSA and Azide free (ab271870)
Formalin-fixed, paraffin-embedded human breast tissue stained for Nestin using Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389 at 1/100 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - BSA and Azide free (ab271870)
Formalin-fixed, paraffin-embedded human skin tissue stained for Nestin using Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389 at 1/100 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - BSA and Azide free (ab271870)
Formalin-fixed, paraffin-embedded human cerebellum tissue stained for Nestin using Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389 at 1/100 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - BSA and Azide free (ab271870)
Formalin-fixed, paraffin-embedded human cervix tissue stained for Nestin using Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389 at 1/100 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - BSA and Azide free (ab271870)
Formalin-fixed, paraffin-embedded human kidney tissue stained for Nestin using Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389 at 1/100 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - BSA and Azide free (ab271870)
Formalin-fixed, paraffin-embedded human ovary tissue stained for Nestin using Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389 at 1/100 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389).