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AB322274

Anti-Nestin antibody [SP103] – Mouse IgG1 (Chimeric) - Neural Stem Cell Marker

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Rabbit Recombinant Monoclonal Nestin antibody. Suitable for ICC/IF and reacts with Human samples.

View Alternative Names

Nbla00170, NES, Nestin

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [SP103] – Mouse IgG1 (Chimeric) - Neural Stem Cell Marker (AB322274)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [SP103] – Mouse IgG1 (Chimeric) - Neural Stem Cell Marker (AB322274)

ab322274 staining Glucagon in Hap1 (positive) and Hap1-NES (negative) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab322274 at 1µg/ml (shown in green) and ab202272, Alexa Fluor® 594 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (shown in pseudocolour magenta). Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 4% paraformaldehyde (10 min).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Unconjugated

    Anti-Nestin antibody [SP103] - Neural Stem Cell Marker

  • Carrier free

    Anti-Nestin antibody [SP103] - BSA and Azide free

  • 660 APC

    APC Anti-Nestin antibody [SP103] - Neural Stem Cell Marker

  • 578 PE

    PE Anti-Nestin antibody [SP103] - Neural Stem Cell Marker

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Nestin antibody [SP103] - Neural Stem Cell Marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Nestin antibody [SP103]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Nestin antibody [SP103] - Neural Stem Cell Marker

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Nestin antibody [SP103] - Neural Stem Cell Marker

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Nestin antibody [SP103] - Neural Stem Cell Marker

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Nestin antibody [SP103] - Neural Stem Cell Marker

  • Carrier free

    Anti-Nestin antibody [SP103] – Mouse IgG1 (Chimeric) – BSA and Azide Free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP103

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }
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Product details

This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab105389). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).
See full target information NES
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com