Rabbit Recombinant Monoclonal Nestin antibody. Suitable for ICC/IF and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [SP103] – Mouse IgG1 (Chimeric) - Neural Stem Cell Marker (AB322274), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-mouse-igg1-chimeric-neural-stem-cell-marker-ab322274--immunocytochemistry-immunofluorescence-img440865.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [SP103] – Mouse IgG1 (Chimeric) - Neural Stem Cell Marker (AB322274)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Target data
Function
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).
Alternative names
Nbla00170, NES, Nestin
Rabbit Recombinant Monoclonal Nestin antibody. Suitable for ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- SP103
- Purification technique
- Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [SP103] – Mouse IgG1 (Chimeric) - Neural Stem Cell Marker (ab322274), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-mouse-igg1-chimeric-neural-stem-cell-marker-ab322274--immunocytochemistry-immunofluorescence-img440865.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [SP103] – Mouse IgG1 (Chimeric) - Neural Stem Cell Marker (ab322274)")
Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [SP103] – Mouse IgG1 (Chimeric) - Neural Stem Cell Marker (ab322274)
Nestin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-Nestin antibody
ab322274 staining Glucagon in Hap1 (positive) and Hap1-NES (negative) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab322274 at 1µg/ml (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272, Alexa Fluor® 594 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (shown in pseudocolour magenta). Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Downloads
Product protocols
- Visit the general protocols
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