Anti-Nestin antibody [SP103] (ab105389) is a rabbit monoclonal antibody detecting Nestin in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, IHC-Fr, ICC/IF. Suitable for Human.
- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 70 publications
Images
![Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (AB105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-frozen-sections-img180042.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (AB105389)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (AB105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img29066.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (AB105389)")
![Western blot - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (AB105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--western-blot-img105237.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (AB105389)")
![Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (AB105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunocytochemistry-immunofluorescence-img82260.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (AB105389)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (AB105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img10767.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (AB105389)")
Publications
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- PeerJ 11:e163582023Comparative study of the efficacy of intra-arterial and intravenous transplantation of human induced pluripotent stem cells-derived neural progenitor cells in experimental stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElvira Cherkashova,Daria Namestnikova,Georgiy Leonov,Ilya Gubskiy,Kirill Sukhinich,Pavel Melnikov,Vladimir Chekhonin,Konstantin Yarygin,Dmitry Goldshtein,Diana SalikhovaPubMed 38025691
- Materials today. Bio 23:1008542023Aptamer-functionalized hydrogels promote bone healing by selectively recruiting endogenous bone marrow mesenchymal stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiang-Shan Gong,Guo-Qiang Zhu,Yu Zhang,Bei Chen,Yi-Wei Liu,Hong-Ming Li,Ze-Hui He,Jing-Tao Zou,Yu-Xuan Qian,Sheng Zhu,Xin-Yue Hu,Shan-Shan Rao,Jia Cao,Hui Xie,Zhen-Xing Wang,Wei DuPubMed 38024846
- Scientific reports 13:178742023Neuroprotective potential of intranasally delivered L-myc immortalized human neural stem cells in female rats after a controlled cortical impact injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMari Amirbekyan,Vikram Adhikarla,Jeffrey P Cheng,Eleni H Moschonas,Corina O Bondi,Russell C Rockne,Anthony E Kline,Margarita GutovaPubMed 37857701
- Cancer cell international 23:1322023CircGNB1 facilitates the malignant phenotype of GSCs by regulating miR-515-5p/miR-582-3p-XPR1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinpeng Hu,Guoqing Zhang,Yongfeng Wang,Kai Xu,Lian Chen,Gang Luo,Jinkun Xu,Hao Li,Dongmei Pei,Xiang Zhao,Zhengting Guo,Xinqiao Li,Shengliang Zong,Yang Jiang,Zhitao JingPubMed 37407973
- Data in brief 47:1089472023Data of MSCs combined with LITUS treatment to improve cognitive impairment in a moderate traumatic brain injury model in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinyu Yao,Wenzhu Wang,Yue Li,Zhendong Cao,Yongheng Wang,Yi Yuan,Xiaoling Li,Xin Liang,Lanxiang Liu,Yan YuPubMed 36819903
- Neurotoxicity research 41:41-562023Catalpol Mitigates Alzheimer's Disease Progression by Promoting the Expression of Neural Stem Cell Exosomes Released miR-138-5p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShengxi Meng,Huize Chen,Chunjun Deng,Zeyu MengPubMed 36595161
- MedComm 4:e1982022SOX2-positive retinal stem cells are identified in adult human pars plicata by single-cell transcriptomic analyses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaotang Wang,Wei Fan,Zongren Xu,Qi Zhang,Na Li,Ruonan Li,Guoqing Wang,Siyuan He,Wanqian Li,Dan Liao,Zhi Zhang,Nan Shu,Jiaxing Huang,Chenyang Zhao,Shengping HouPubMed 36582303
- Journal of biomedical materials research. Part B, Applied biomaterials 111:551-5592022Decellularized fish swim bladder patch loaded with mesenchymal stem cells inhibits neointimal hyperplasia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Sun,Haoliang Wu,Xiche Bai,Liwei Zhang,Cong Zhang,Xueyun Wang,Chunyang Lou,Bo Li,Zhuo Li,Hualong BaiPubMed 36200602
- Nature 610:190-1982022A cellular hierarchy in melanoma uncouples growth and metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPanagiotis Karras,Ignacio Bordeu,Joanna Pozniak,Ada Nowosad,Cecilia Pazzi,Nina Van Raemdonck,Ewout Landeloos,Yannick Van Herck,Dennis Pedri,Greet Bervoets,Samira Makhzami,Jia Hui Khoo,Benjamin Pavie,Jochen Lamote,Oskar Marin-Bejar,Michael Dewaele,Han Liang,Xingju Zhang,Yichao Hua,Jasper Wouters,Robin Browaeys,Gabriele Bergers,Yvan Saeys,Francesca Bosisio,Joost van den Oord,Diether Lambrechts,Anil K Rustgi,Oliver Bechter,Cedric Blanpain,Benjamin D Simons,Florian Rambow,Jean-Christophe MarinePubMed 36131018
- Journal of oncology 2022:64354312022Chelerythrine Inhibits Stemness of Cancer Stem-Like Cells of Osteosarcoma and PI3K/AKT/mTOR Signal.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhixing Chen,Hui Yang,Qianlu Zhang,Qiongying Hu,Ziyi ZhaoPubMed 36131794
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | WB | IHC-P | ICC/IF | IHC-Fr | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Dog | Predicted | Predicted | Predicted | Predicted | Predicted |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Antigen retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).
Alternative names
Nbla00170, NES, Nestin
Recommended products
Anti-Nestin antibody [SP103] (ab105389) is a rabbit monoclonal antibody detecting Nestin in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, IHC-Fr, ICC/IF. Suitable for Human.
- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 70 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- SP103
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from TCS by protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Nestin antibody [SP103] (ab105389) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, WB in human samples.
Anti-Nestin antibody [SP103] (ab105389) specifically detects Nestin (UniProt ID: P48681; Molecular weight: 177kDa) and is sold in 100 µL, 500 µL and 1 mL selling sizes.
Anti-Nestin antibody [SP103] (ab105389) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-Nestin antibody [SP103] (ab105389) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-Nestin antibody [SP103] (ab105389) has been confirmed by testing in knockout samples.
Anti-Nestin antibody [SP103] (ab105389) has been cited over 72 times in peer reviewed journals and is trusted by the scientific community.
Anti-Nestin antibody [SP103] (ab105389) has 7 independent reviews from customers.
Related Products
Antibody clone SP103 is also available pre-conjugated to a variety of labels for your convenience - APC, PE, Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 594, Alexa Fluor® 568, Alexa Fluor® 555, Alexa Fluor® 750 (APC Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab310807, PE Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab310889, Alexa Fluor® 488 Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab310984, Alexa Fluor® 647 Anti-Nestin antibody [SP103] ab311106, Alexa Fluor® 594 Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab311725, Alexa Fluor® 568 Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab313002, Alexa Fluor® 555 Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab313206, Alexa Fluor® 750 Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab320885).
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
17 product images
![Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-frozen-sections-img180042.jpg "Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)
Nestin Immunohistochemistry (Frozen sections) staining using rabbit Anti-Nestin antibody
IHC image of Nestin staining in a section of frozen normal human kidney* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab105389, 5ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img29066.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
Malchenko, S. et al PLoS One. 2017 Mar 1;12(3):e0173106. doi: 10.1371/journal.pone.0173106. eCollection 2017 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)
Nestin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Nestin antibody
BTIC markers in our CNS-PNET model tumorsFormalin-fixed, paraffin-embedded mouse primitive neural ectodermal tumor tissue was stained for Nestin using ab105389 at 1/30 dilution in immunohistochemical analysis.
Insert x20 magnification.
TU-tumor; P-parenchyma; V-ventricle; CP-choroid plexus
(From Figure 3C of Malchenko et al)
![Western blot - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--western-blot-img105237.jpg "Western blot - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
Western blot - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)
Nestin Western blot staining using rabbit Anti-Nestin antibody
Lanes 1 - 2: Merged signal (red and green). Green - ab105389 observed at 300 kDa. Red - loading control, ab18058, observed at 117 kDa.ab105389 was shown to specifically react with NES in wild-type HAP1 cells as signal was lost in NES knockout cells. Wild-type and NES knockout samples were subjected to SDS-PAGE. ab105389 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/100 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389) at 1/100 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: NES knockout HAP1 whole cell lysate at 20 µg
Predicted band size: 177 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunocytochemistry-immunofluorescence-img82260.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)
Nestin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-Nestin antibody
ICC/IF image of ab105389 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105389, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img10767.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)
Nestin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Nestin antibody
Formalin-fixed, paraffin-embedded human endometrium tissue stained for Nestin using ab105389 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135811.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
This image is courtesy of Hongwei Shao by customer review.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)
Nestin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Nestin antibody
ab105389 staining Nestin in human melanoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using Tris EDTA pH 9.0. Samples were then permeabilized using 0.25% Triton X-100, blocked, then incubated with ab105389 at a 1/100 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 594 (red) conjugated donkey anti-rabbit polyclonal used at a 1/100 dilution. (Green) tumor cells, (blue) DAPI.![Western blot - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--western-blot-img25851.jpg "Western blot - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
Western blot - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)
Nestin Western blot staining of HeLa cell lysate using rabbit Anti-Nestin antibody
All lanes: Western blot - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389) at 1/100 dilution
All lanes: HeLa cell lysate
Predicted band size: 177 kDa
![Flow Cytometry (Intracellular) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--flow-cytometry-intracellular-img89527.jpg "Flow Cytometry (Intracellular) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
Flow Cytometry (Intracellular) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)
Nestin Flow Cytometry (Intracellular) staining using rabbit Anti-Nestin antibody
Overlay histogram showing SH-SY5Y cells stained with ab105389 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab105389, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img27891.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)
Nestin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Nestin antibody
Formalin-fixed, paraffin-embedded human colon tissue stained for Nestin using ab105389 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109456.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)
Nestin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Nestin antibody
Formalin-fixed, paraffin-embedded human prostate tissue stained for Nestin using ab105389 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130095.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)
Nestin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Nestin antibody
Formalin-fixed, paraffin-embedded human lung tissue stained for Nestin using ab105389 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82937.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)
Nestin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Nestin antibody
Formalin-fixed, paraffin-embedded human breast tissue stained for Nestin using ab105389 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25905.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)
Nestin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Nestin antibody
Formalin-fixed, paraffin-embedded human skin tissue stained for Nestin using ab105389 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img96807.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)
Nestin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Nestin antibody
Formalin-fixed, paraffin-embedded human cerebellum tissue stained for Nestin using ab105389 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img14980.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)
Nestin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Nestin antibody
Formalin-fixed, paraffin-embedded human cervix tissue stained for Nestin using ab105389 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112976.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)
Nestin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Nestin antibody
Formalin-fixed, paraffin-embedded human kidney tissue stained for Nestin using ab105389 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104796.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)
Nestin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Nestin antibody
Formalin-fixed, paraffin-embedded human ovary tissue stained for Nestin using ab105389 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135811.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
![Western blot - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--western-blot-img25851.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
![Flow Cytometry (Intracellular) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--flow-cytometry-intracellular-img89527.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img27891.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109456.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130095.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82937.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25905.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img96807.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img14980.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112976.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389), expandable thumbnail](https://content.abcam.com/products/images/nestin-antibody-sp103-neural-stem-cell-marker-ab105389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104796.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (ab105389)")
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