AB109288

Anti-NETO2 antibody [EPR3497]

RabMAb
Recombinant
What is this?

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(6 Publications)

Rabbit Recombinant Monoclonal NETO2 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 6 publications.

View Alternative Names

BTCL2, UNQ1926/PRO4401, NETO2, Neuropilin and tolloid-like protein 2, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 2

2 Images

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Western blot - Anti-NETO2 antibody [EPR3497] (AB109288)

All lanes:

Western blot - Anti-NETO2 antibody [EPR3497] (ab109288) at 1/1000 dilution

Lane 1:

Human cerebellum lysates at 10 µg

Lane 2:

SH-SY5Y cell lysates at 10 µg

Lane 3:

Human brain lysates at 10 µg

Predicted band size: 59 kDa

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Western blot - Anti-NETO2 antibody [EPR3497] (AB109288)

All lanes:

Western blot - Anti-NETO2 antibody [EPR3497] (ab109288) at 1/1000 dilution

Lane 1:

mouse brain lysates at 10 µg

Lane 2:

rat brain lysates at 10 µg

Predicted band size: 101 kDa,118 kDa,140 kDa,15 kDa,45 kDa,51 kDa,56 kDa,59 kDa,63 kDa,92 kDa

Observed band size: 101 kDa,110 kDa,140 kDa,15 kDa,49 kDa,56 kDa,92 kDa

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Carrier free

Anti-NETO2 antibody [EPR3497] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3497

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.31% Sodium citrate, 0.175% Sodium chloride, 0.05% BSA, 0.0172% Ethylenediaminetetraacetic acid, disodium, dihydrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NETO2 also known as Neuropilin and Tolloid-Like 2 is a transmembrane protein with a known mass of approximately 58 kDa. This protein functions mechanically as an auxiliary subunit for kainate receptors in the central nervous system. Researchers observe its expression predominantly in neurons but also in some other tissues. NETO2 alongside its variants impacts receptor functionality by modulating properties like receptor trafficking and kinetic attributes.

Biological function summary

The protein affects synaptic plasticity and neuronal communication. NETO2 forms part of a receptor complex with kainate receptors influencing synaptic transmission processes. This association is significant for excitatory neurotransmission which is important for learning and memory. Researchers emphasize the role of NETO2 in maintaining neuronal circuit efficiency and integrity.

Pathways

NETO2 involvement strongly ties to glutamatergic synapse pathways. It influences neuronal signaling through kainate receptor modulation which in turn affects calcium ion permeation. NETO2 also interacts with other proteins like GRIP1 (Glutamate Receptor Interacting Protein 1) within these pathways orchestrating synaptic stability and function.

Researchers link NETO2 alterations mainly to psychiatric disorders such as schizophrenia and neurodevelopmental disorders. In these contexts aberrant interactions with proteins like PSD95 (Postsynaptic Density Protein 95) could disrupt normal synaptic functioning. Researchers continue to investigate how dysregulation in NETO2 contributes to the molecular pathways underlying these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Accessory subunit of neuronal kainate-sensitive glutamate receptors, GRIK2 and GRIK3. Increases kainate-receptor channel activity, slowing the decay kinetics of the receptors, without affecting their expression at the cell surface, and increasing the open probability of the receptor channels. Modulates the agonist sensitivity of kainate receptors. Slows the decay of kainate receptor-mediated excitatory postsynaptic currents (EPSCs), thus directly influencing synaptic transmission (By similarity).

See full target information NETO2

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy oncolytics 24:535-546 PubMed35229031

2022

TTN-AS1 accelerates the growth and migration of nasopharyngeal carcinoma cells via targeting miR-876-5p/NETO2.

Applications

Unspecified application

Species

Unspecified reactive species

Xinping Chen,Weihua Xu,Zhichao Ma,Juan Zhu,Junjie Hu,Xiaojuan Li,Shengmiao Fu

Oncology letters 22:818 PubMed34671432

2021

Long non-coding RNA SNHG17 promotes lung adenocarcinoma progression by targeting the microRNA-193a-5p/NETO2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwei Zhang,Yulan Yan,Bin Zhang,Yuchen Ma,Chen Chen,Changli Wang

Cell reports 31:107735 PubMed32521260

2020

Kainate Receptor Activation Shapes Short-Term Synaptic Plasticity by Controlling Receptor Lateral Mobility at Glutamatergic Synapses.

Applications

Unspecified application

Species

Unspecified reactive species

Alice Polenghi,Thierry Nieus,Stefania Guazzi,Pau Gorostiza,Enrica Maria Petrini,Andrea Barberis

The Journal of biological chemistry 292:15369-15377 PubMed28717010

2017

Phosphorylation of the kainate receptor (KAR) auxiliary subunit Neto2 at serine 409 regulates synaptic targeting of the KAR subunit GluK1.

Applications

Unspecified application

Species

Unspecified reactive species

Richa Madan Lomash,Nengyin Sheng,Yan Li,Roger A Nicoll,Katherine W Roche

The Journal of neuroscience : the official journal 37:3352-3363 PubMed28235897

2017

Neto2 Assembles with Kainate Receptors in DRG Neurons during Development and Modulates Neurite Outgrowth in Adult Sensory Neurons.

Applications

Species

Unspecified reactive species

Claire G Vernon,Geoffrey T Swanson

BMC cancer 15:1006 PubMed26699544

2015

Upregulation of NETO2 expression correlates with tumor progression and poor prognosis in colorectal carcinoma.

Applications

Species

Human

Liang Hu,Hai-Yang Chen,Jian Cai,Guang-Zhen Yang,Dan Feng,Yan-Xia Zhai,Hui Gong,Chen-Ye Qi,Yu Zhang,Hao Fu,Qing-Ping Cai,Chun-Fang Gao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NETO2 antibody [EPR3497]

Western blot - Anti-NETO2 antibody [EPR3497] (AB109288)

Western blot - Anti-NETO2 antibody [EPR3497] (AB109288)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise