AB247819

Anti-NETO2 antibody [EPR3497] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal NETO2 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human, Rat samples.

View Alternative Names

BTCL2, UNQ1926/PRO4401, NETO2, Neuropilin and tolloid-like protein 2, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 2

2 Images

Unknown

Western blot - Anti-NETO2 antibody [EPR3497] - BSA and Azide free (AB247819)

This data was developed using ab109288, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NETO2 antibody [EPR3497] (<a href='/en-us/products/primary-antibodies/neto2-antibody-epr3497-ab109288'>ab109288</a>) at 1/1000 dilution

Lane 1:

Human cerebellum lysates at 10 µg

Lane 2:

SH-SY5Y cell lysates at 10 µg

Lane 3:

Human brain lysates at 10 µg

Predicted band size: 59 kDa

false

Unknown

Western blot - Anti-NETO2 antibody [EPR3497] - BSA and Azide free (AB247819)

This data was developed using ab109288, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NETO2 antibody [EPR3497] (<a href='/en-us/products/primary-antibodies/neto2-antibody-epr3497-ab109288'>ab109288</a>) at 1/1000 dilution

Lane 1:

mouse brain lysates at 10 µg

Lane 2:

rat brain lysates at 10 µg

Predicted band size: 101 kDa,118 kDa,140 kDa,15 kDa,45 kDa,51 kDa,56 kDa,59 kDa,63 kDa,92 kDa

Observed band size: 101 kDa,110 kDa,140 kDa,15 kDa,49 kDa,56 kDa,92 kDa

false

Unconjugated

Anti-NETO2 antibody [EPR3497]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3497

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab247819 is the carrier-free version of ab109288.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NETO2 also known as Neuropilin and Tolloid-Like 2 is a transmembrane protein with a known mass of approximately 58 kDa. This protein functions mechanically as an auxiliary subunit for kainate receptors in the central nervous system. Researchers observe its expression predominantly in neurons but also in some other tissues. NETO2 alongside its variants impacts receptor functionality by modulating properties like receptor trafficking and kinetic attributes.

Biological function summary

The protein affects synaptic plasticity and neuronal communication. NETO2 forms part of a receptor complex with kainate receptors influencing synaptic transmission processes. This association is significant for excitatory neurotransmission which is important for learning and memory. Researchers emphasize the role of NETO2 in maintaining neuronal circuit efficiency and integrity.

Pathways

NETO2 involvement strongly ties to glutamatergic synapse pathways. It influences neuronal signaling through kainate receptor modulation which in turn affects calcium ion permeation. NETO2 also interacts with other proteins like GRIP1 (Glutamate Receptor Interacting Protein 1) within these pathways orchestrating synaptic stability and function.

Researchers link NETO2 alterations mainly to psychiatric disorders such as schizophrenia and neurodevelopmental disorders. In these contexts aberrant interactions with proteins like PSD95 (Postsynaptic Density Protein 95) could disrupt normal synaptic functioning. Researchers continue to investigate how dysregulation in NETO2 contributes to the molecular pathways underlying these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Accessory subunit of neuronal kainate-sensitive glutamate receptors, GRIK2 and GRIK3. Increases kainate-receptor channel activity, slowing the decay kinetics of the receptors, without affecting their expression at the cell surface, and increasing the open probability of the receptor channels. Modulates the agonist sensitivity of kainate receptors. Slows the decay of kainate receptor-mediated excitatory postsynaptic currents (EPSCs), thus directly influencing synaptic transmission (By similarity).

See full target information NETO2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NETO2 antibody [EPR3497] - BSA and Azide free

Western blot - Anti-NETO2 antibody [EPR3497] - BSA and Azide free (AB247819)

Western blot - Anti-NETO2 antibody [EPR3497] - BSA and Azide free (AB247819)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise