AB122903

Anti-Netrin 1 antibody

Name: Anti-Netrin 1 antibody (ab122903) | Abcam
Brand: Abcam Limited
SKU: AB122903

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(7 Publications)

Goat Polyclonal Netrin 1 antibody. Suitable for WB, IHC-P and reacts with Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human NTN1 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

NTN1L, NTN1, Netrin-1, Epididymis tissue protein Li 131P

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Netrin 1 antibody (AB122903)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Netrin 1 antibody (AB122903)

ab122903, at 3.75 µg/ml, staining Netrin 1 in Formalin-fixed, Paraffin-embedded Human Intestine tissue by Immunohistochemistry.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human NTN1 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O95631

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3.75 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1.5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Netrin-1 also known as NTN1 or Netrin-1 protein is a guidance cue important for cell migrations. It falls under the netrin proteins and has an approximate mass of 70 kDa. This protein is mainly expressed in the central nervous system especially during development but also appears in certain developing tissues and organs. Netrin-1 interacts with various receptors influencing axon guidance and cell motility.

Biological function summary

Netrin proteins play key roles in directing neuronal and vascular development. Netrin-1 influences axon pathfinding by attracting or repelling growth cones and also participates in angiogenesis. It does not usually form a stable complex but functions through interactions with distinct receptor types like DCC Neogenin and UNC5 homologue proteins. These interactions determine the direction of cellular movement and position within tissues.

Pathways

Netrin-1 integrates within signaling pathways such as the MAPK and PI3K/AKT pathways. It influences neuron migration and blood vessel formation within these pathways. Netrin receptors like DCC and UNC5B are important in transducing netrin-1 signals. These pathways involve intricate signaling cascades modulating cell survival growth and trajectory establishing netrin-1 as significant in neurodevelopmental processes.

Researchers link netrin-1 to neurodegenerative diseases and cancer metastasis. Abnormal levels of netrin-1 can associate with disorders like Alzheimer's disease and various cancers. In cancer netrin-1 interaction with its receptors might influence tumour cell survival and invasion resembling mechanisms found in neuronal cells. Understanding the role of netrin-1 in these conditions could uncover valuable therapeutic targets potentially involving DCC and UNC5 homologues among other associated molecules.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. Binding to UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed : 28483977). Involved in dorsal root ganglion axon projection towards the spinal cord (PubMed : 28483977). It also serves as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis (PubMed : 15343335).

See full target information NTN1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

BMC gastroenterology 24:308 PubMed39261771

2024

Netrin-1-CD146 and netrin-1-S100A9 are associated with early stage of lymph node metastasis in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Ming Chen,Jun He,Jian-Ming Qiu,Guan-Gen Yang,Dong Wang,Zhong Shen

Biological psychiatry 95:37-47 PubMed37355003

2023

Gains and Losses: Resilience to Social Defeat Stress in Adolescent Female Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Harée Pantoja-Urbán,Samuel Richer,Amelie Mittermaier,Michel Giroux,Dominique Nouel,Giovanni Hernandez,Cecilia Flores

Cartilage 13:94-104 PubMed36321743

2022

Netrin-1 Secreted by Human Osteoarthritic Articular Chondrocytes Promotes Angiogenesis .

Applications

Unspecified application

Species

Unspecified reactive species

Joulnar Akoum,Marie-Thérèse Corvol,Khadija Tahiri,Philippe Anract,David Biau,Didier Borderie,François Étienne,François Rannou,Christelle Nguyen

Annals of translational medicine 9:29 PubMed33553322

2021

Macrophage-derived netrin-1 contributes to endometriosis-associated pain.

Applications

Unspecified application

Species

Unspecified reactive species

Shaojie Ding,Xinyue Guo,Libo Zhu,Jianzhang Wang,Tiantian Li,Qin Yu,Xinmei Zhang

Journal of Cancer 10:3197-3207 PubMed31289590

2019

Netrin-1 promotes cell neural invasion in gastric cancer via its receptor neogenin.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Yin,Linjun Wang,Yiwen Xia,Shengchun Dang,Xuan Zhang,Zhongyuan He,Jianghao Xu,Mengyuan Shang,Zekuan Xu

Frontiers in cellular neuroscience 11:432 PubMed29375318

2018

Neuroprotection Exerted by Netrin-1 and Kinesin Motor KIF1A in Secondary Brain Injury following Experimental Intracerebral Hemorrhage in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wang,Weiwei Zhai,Zhengquan Yu,Liang Sun,Haiying Li,Haitao Shen,Xiang Li,Chunfeng Liu,Gang Chen

Oncotarget 7:24719-33 PubMed27034160

2016

Netrin-1 suppresses the MEK/ERK pathway and ITGB4 in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xi-Zhou An,Zhi-Guo Zhao,Yu-Xuan Luo,Ran Zhang,Xiao-Qiang Tang,De- Long Hao,Xiang Zhao,Xiang Lv,De- Pei Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Netrin 1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Netrin 1 antibody (AB122903)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise