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Rabbit Recombinant Monoclonal Netrin 1 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

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Images

Western blot - Anti-Netrin 1 antibody [EPR5428] - BSA and Azide free (AB201324), expandable thumbnail
  • Western blot - Anti-Netrin 1 antibody [EPR5428] - BSA and Azide free (AB201324), expandable thumbnail
  • Western blot - Anti-Netrin 1 antibody [EPR5428] - BSA and Azide free (AB201324), expandable thumbnail
  • Western blot - Anti-Netrin 1 antibody [EPR5428] - BSA and Azide free (AB201324), expandable thumbnail
  • Immunoprecipitation - Anti-Netrin 1 antibody [EPR5428] - BSA and Azide free (AB201324), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IF
Human
Tested
Expected
Not recommended
Not recommended
Mouse
Predicted
Expected
Not recommended
Not recommended
Rat
Predicted
Expected
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. Binding to UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Involved in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977). It also serves as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis (PubMed:15343335).

Alternative names

NTN1L, NTN1, Netrin-1, Epididymis tissue protein Li 131P

Recommended products

Rabbit Recombinant Monoclonal Netrin 1 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR5428
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab201324 is the carrier-free version of Anti-Netrin 1 antibody [EPR5428] ab126729.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Netrin-1 also known as NTN1 or Netrin-1 protein is a guidance cue important for cell migrations. It falls under the netrin proteins and has an approximate mass of 70 kDa. This protein is mainly expressed in the central nervous system especially during development but also appears in certain developing tissues and organs. Netrin-1 interacts with various receptors influencing axon guidance and cell motility.

Biological function summary

Netrin proteins play key roles in directing neuronal and vascular development. Netrin-1 influences axon pathfinding by attracting or repelling growth cones and also participates in angiogenesis. It does not usually form a stable complex but functions through interactions with distinct receptor types like DCC Neogenin and UNC5 homologue proteins. These interactions determine the direction of cellular movement and position within tissues.

Pathways

Netrin-1 integrates within signaling pathways such as the MAPK and PI3K/AKT pathways. It influences neuron migration and blood vessel formation within these pathways. Netrin receptors like DCC and UNC5B are important in transducing netrin-1 signals. These pathways involve intricate signaling cascades modulating cell survival growth and trajectory establishing netrin-1 as significant in neurodevelopmental processes.

Associated diseases and disorders

Researchers link netrin-1 to neurodegenerative diseases and cancer metastasis. Abnormal levels of netrin-1 can associate with disorders like Alzheimer's disease and various cancers. In cancer netrin-1 interaction with its receptors might influence tumour cell survival and invasion resembling mechanisms found in neuronal cells. Understanding the role of netrin-1 in these conditions could uncover valuable therapeutic targets potentially involving DCC and UNC5 homologues among other associated molecules.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-Netrin 1 antibody [EPR5428] - BSA and Azide free (ab201324), expandable thumbnail

    Western blot - Anti-Netrin 1 antibody [EPR5428] - BSA and Azide free (ab201324)

    This data was developed using Anti-Netrin 1 antibody [EPR5428] ab126729, the same antibody clone in a different buffer formulation.

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-Netrin 1 antibody [EPR5428] (Anti-Netrin 1 antibody [EPR5428] ab126729) at 1/500 dilution

    Lane 1: Rat brain lysates prepared in RIPA lysis method at 20 µg

    Lane 2: Rat brain lysates prepared in 1%SDS Hot lysis method at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 67 kDa

    Observed band size: 75 kDa

    Exposure time: 180s

  • Western blot - Anti-Netrin 1 antibody [EPR5428] - BSA and Azide free (ab201324), expandable thumbnail

    Western blot - Anti-Netrin 1 antibody [EPR5428] - BSA and Azide free (ab201324)

    This data was developed using Anti-Netrin 1 antibody [EPR5428] ab126729, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Netrin 1 antibody [EPR5428] (Anti-Netrin 1 antibody [EPR5428] ab126729) at 1/1000 dilution

    Lane 1: Mouse kidney tissue lysate at 20 µg

    Lane 2: Mouse heart tissue lysate at 20 µg

    Lane 3: Rat kidney tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 67 kDa

    Observed band size: 75 kDa

  • Western blot - Anti-Netrin 1 antibody [EPR5428] - BSA and Azide free (ab201324), expandable thumbnail

    Western blot - Anti-Netrin 1 antibody [EPR5428] - BSA and Azide free (ab201324)

    This data was developed using Anti-Netrin 1 antibody [EPR5428] ab126729, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Netrin 1 antibody [EPR5428] (Anti-Netrin 1 antibody [EPR5428] ab126729) at 1/1000 dilution

    All lanes: Human fetal brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 67 kDa

    Observed band size: 75 kDa

  • Western blot - Anti-Netrin 1 antibody [EPR5428] - BSA and Azide free (ab201324), expandable thumbnail

    Western blot - Anti-Netrin 1 antibody [EPR5428] - BSA and Azide free (ab201324)

    This data was developed using Anti-Netrin 1 antibody [EPR5428] ab126729, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Netrin 1 antibody [EPR5428] (Anti-Netrin 1 antibody [EPR5428] ab126729) at 1/1000 dilution

    Lane 1: Human fetal brain lysate at 10 µg

    Lane 2: Human small intestine lysate at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 67 kDa

  • Immunoprecipitation - Anti-Netrin 1 antibody [EPR5428] - BSA and Azide free (ab201324), expandable thumbnail

    Immunoprecipitation - Anti-Netrin 1 antibody [EPR5428] - BSA and Azide free (ab201324)

    This data was developed using Anti-Netrin 1 antibody [EPR5428] ab126729, the same antibody clone in a different buffer formulation.

    Immunoprecipitation of NTN1 in MCF7 cell culture media. Culture medium was prepared and immunoprecipitation was performed using 2 μg of Anti-Netrin 1 antibody [EPR5428] ab126729 pre-coupled to Protein A beads. Samples were then washed and processed for western blot.

    This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

    All lanes: Immunoprecipitation - Anti-Netrin 1 antibody [EPR5428] (Anti-Netrin 1 antibody [EPR5428] ab126729) at 2 µg

  • Western blot - Anti-Netrin 1 antibody [EPR5428] - BSA and Azide free (ab201324), expandable thumbnail

    Western blot - Anti-Netrin 1 antibody [EPR5428] - BSA and Azide free (ab201324)

    Netrin 1 Western blot staining using rabbit Anti-Netrin 1 antibody

    This data was developed using Anti-Netrin 1 antibody [EPR5428] ab126729, the same antibody clone in a different buffer formulation.

    Anti-Netrin 1 antibody [EPR5428] ab126729 was shown to react with NTN1 in wild-type MCF7 cell culture media in Western blot with loss of signal observed in culture media from NTN1 knockout cell line Human NTN1 knockout MCF7 cell line ab323856. Wild-type MCF7 and NTN1 knockout cell culture medium was subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with Anti-Netrin 1 antibody [EPR5428] ab126729 overnight at 4 °C at a 1/2000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

    This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

    All lanes: Western blot - Anti-Netrin 1 antibody [EPR5428] (Anti-Netrin 1 antibody [EPR5428] ab126729) at 1/2000 dilution

    Lane 1: Wild-type MCF7 cell culture medium at 10 µg

    Lane 2: NTN1 knock-out MCF7 cell culture medium at 10 µg

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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