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AB185200

Anti-Netrin-3 antibody

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(3 Publications)

Rabbit Polyclonal Netrin-3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NTN3 aa 1-150.

View Alternative Names

NTN2L, NTN3, Netrin-3, Netrin-2-like protein

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Netrin-3 antibody (AB185200)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Netrin-3 antibody (AB185200)

Immunohistochemical analysis of paraffin embedded Human oral mucosa tissue labeling Netrin-3 with ab185200 at 1/50 and counterstained with hematoxylin.

Western blot - Anti-Netrin-3 antibody (AB185200)
  • WB

Supplier Data

Western blot - Anti-Netrin-3 antibody (AB185200)

All lanes:

Western blot - Anti-Netrin-3 antibody (ab185200) at 1/250 dilution

Lane 1:

RT 4 cell line lysate

Lane 2:

U 251MG cell line lysate

Lane 3:

Human plasma (IgG/HSA depleted)

Secondary

All lanes:

Swine anti-rabbit HRP conjugated antibody at 1/3000 dilution

Predicted band size: 61 kDa

true

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NTN3 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

O00634

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Netrin-3 also known as NTN3 is a member of the netrin family of proteins and plays a role in guiding axonal growth during neural development. This protein has an approximate molecular mass of 65 kDa. Scientists have observed Netrin-3 expression mainly in the nervous system including the central and peripheral nervous tissues. In addition to nervous tissues it shows presence in various other tissues such as the pancreas and the lungs indicating a wider range of functional implications.
Biological function summary

Netrin-3 helps with cellular movement and positioning in the developing nervous system. It is not part of a larger protein complex but acts through interactions with its receptors like DCC (Deleted in Colorectal Cancer) and neogenin near the cell surface. These interactions facilitate not only guidance of axons but also influence cell adhesion processes impacting how cells navigate during development.

Pathways

Netrin-3 is actively involved in at least two critical pathways: the axon guidance pathway and the cell adhesion pathway. In the axon guidance pathway Netrin-3 interacts with proteins such as DCC to direct the movements of neuronal axons. In the cell adhesion pathway it collaborates with neogenin to support cell attachment and migration essential for structuring tissues during development. These interactions show that Netrin-3 plays important roles in shaping cellular architecture.

Abnormal function or expression of Netrin-3 connects to neurological disorders like schizophrenia and neurodevelopmental disorders. The relationship with proteins like DCC and neogenin can influence the onset of these conditions. Alterations in Netrin-3 levels can disrupt the normal pathways impacting neural development and leading to these complex disorders. Understanding its role opens avenues for potential therapeutic strategies targeting these pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Netrins control guidance of CNS commissural axons and peripheral motor axons.
See full target information NTN3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed39023520

2024

Netrin signaling mediates survival of dormant epithelial ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Pirunthan Perampalam,James I MacDonald,Komila Zakirova,Daniel T Passos,Sumaiyah Wasif,Yudith Ramos-Valdes,Maeva Hervieu,Patrick Mehlen,Rob Rottapel,Benjamin Gibert,Rohann J M Correa,Trevor G Shepherd,Frederick A Dick

EMBO molecular medicine 13:e12878 PubMed33719214

2021

Targeting netrin-3 in small cell lung cancer and neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Jiang,Mathieu Richaud,Pauline Vieugué,Nicolas Rama,Jean-Guy Delcros,Maha Siouda,Mitsuaki Sanada,Anna-Rita Redavid,Benjamin Ducarouge,Maëva Hervieu,Silvia Breusa,Ambroise Manceau,Charles-Henry Gattolliat,Nicolas Gadot,Valérie Combaret,David Neves,Sandra Ortiz-Cuaran,Pierre Saintigny,Olivier Meurette,Thomas Walter,Isabelle Janoueix-Lerosey,Paul Hofman,Peter Mulligan,David Goldshneider,Patrick Mehlen,Benjamin Gibert

Nature 545:350-354 PubMed28445456

2017

Floor-plate-derived netrin-1 is dispensable for commissural axon guidance.

Applications

Unspecified application

Species

Unspecified reactive species

Chloé Dominici,Juan Antonio Moreno-Bravo,Sergi Roig Puiggros,Quentin Rappeneau,Nicolas Rama,Pauline Vieugue,Agnes Bernet,Patrick Mehlen,Alain Chédotal
View all publications
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